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Changes of Fruit Structure and Physiological Metabolism and Roles of Chemical Regulator under Low Temperature Stress

Author: HanJin
Tutor: TianShiPing
School: Institute of Botany
Course: Cell Biology
Keywords: Chilling injury Fruit Methyl salicylate Methyl jasmonate Cell wall structural components
CLC: S601
Type: Master's thesis
Year: 2006
Downloads: 376
Quote: 0
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Abstract


Cold Storage horticultural products delay ripening, inhibit the growth of pathogenic bacteria and maintain the quality of conventional methods. However, many horticultural products is quite sensitive to low temperature (generally less than 10 oC to 12 oC) if the storage temperature is too low, it is easy to produce cold damage, reduce the commercial value. Research postharvest chilling injury mechanism of horticultural products and how to improve its cold resistance of potential economic value of scientific problems. Methyl salicylate and methyl jasmonate signal substances of plants, studies have shown that these two substances can alleviate the degree of chilling injury at Low Temperature Storage fruit or fruit the cold resistance, but the mechanism is not clear. In this thesis, mango, peach and cucumber, research methyl salicylate or methyl jasmonate treatment of fruits under the storage conditions of cold damage or non-chilling injury antioxidant metabolism, phenolic metabolism, membrane integrity, and cell wall composition and structure and other aspects of the change, further confirmed by methyl salicylate and methyl jasmonate can alleviate chilling injury, also discussed to improve the cold resistance mechanism. In this thesis, changes in the scanning electron microscope fruit epidermis layer of wax with an optical microscope, transmission electron microscopy and Fourier transform infrared spectroscopy study of fruit cell wall structure and composition changes, changes in the cell wall of calcium by atomic absorption spectrophotometer, the integrity of the fruit cells detected with a conductivity meter. Simultaneous determination of fruit polyphenol content, polyphenol oxidase (EC 1.10.3.1) and peroxidase (EC 1.11.1.7) activity and analysis of fruit firmness, sugar and acid content and quality indicators. The results showed that: suitable concentration of methyl salicylate and methyl jasmonate can alleviate chilling injury symptoms and improve the cold resistance of the fruit. , Methyl salicylate treatment can change the the fruit epidermis layer of wax structure; to reduce epidermal lipids and cell wall phenolic compounds accumulation; inhibit the degradation of cell wall fiber material, adjust the dissolution of pectin substances, to protect the cell wall structure. Methyl jasmonate treatment can protect the integrity of the cell membrane; regulate fruit phenolic metabolism, relieve the enzymatic browning of fruit; inhibit the degradation of pectin substances and fiber material, the maintenance of cell wall structure and firmness, will help improve the fruit cold resistance and alleviate chilling injury. Although the performance of different fruit chilling injury symptoms are not identical, but the low temperature stress on the destruction of the plant tissue structure (membrane and cell wall structure) is the root cause of cause chilling injury. Improve fruit cold resistance of various adjustment mechanism in the final analysis by protecting the normal cell structure play a role. Methyl salicylate and methyl jasmonate treatment to protect the organization structure of the fruit, to alleviate the damage of low temperature stress on fruit, fruit cold resistance. However, the fruits of their own material composition differences is the main cause of chilling injury symptoms.

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CLC: > Agricultural Sciences > Gardening > General issues > Biological principles
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