Dissertation > Excellent graduate degree dissertation topics show

Extraction , purification and immunological activity of lactic acid bacteria exopolysaccharides

Author: WangJingYing
Tutor: WangTianHong
School: Shandong University
Course: Microbiology
Keywords: Lactic acid bacteria Exopolysaccharide Polysaccharide extracted Purification of the Polysaccharide Immune function
CLC: Q939.91
Type: Master's thesis
Year: 2007
Downloads: 629
Quote: 3
Read: Download Dissertation

Abstract


Lactic acid bacteria extracellular polysaccharide is a sugar compound secreted outside the cell wall of lactic acid bacteria in the process of growth and metabolism. Many studies have shown that the anti-tumor immune regulation, and the role of lactic acid bacteria exopolysaccharides on the human body. The paper describes the background information on lactic acid bacteria exopolysaccharides, lactic acid bacteria exopolysaccharides chemical properties, physiological functions, applications and factors affecting its production are reviewed; based on yoghurt-producing bacteria - Bulgaria Lactobacillus EPS produced by the thermophilic Streptococcus lactis produces conditions, extraction and purification as well as immune activity research. First generation conditions of the extracellular polysaccharide in yoghurt production process and found that the use of a smaller inoculation amount 2% lower than the optimal growth temperature (42 ° C), i.e. cultured under conditions of 38 ℃ 2.5h, the end of fermentation low acidity, it is advantageous that the yogurt producing bacteria produce EPS. Juice on extracellular polysaccharide production, the results showed that the tomato juice and four kinds of juice is not only lactic acid bacteria growth-promoting substances, can also increase the production of extracellular polysaccharides. Extracted from yoghurt EPS, removal of protein in the process and the extraction process, the results showed that the enzymatically Sevag except protein 2 method works best. This method not only improve the extraction rate of polysaccharides and organic solvent savings. For further research yoghurt-producing bacteria produce EPS, respectively from Lactobacillus bulgaricus and Streptococcus agalactiae thermophilic MRS culture the exopolysaccharide was extracted, purified. C57 inbred mice, the immune activity of lactic acid bacteria exopolysaccharides experimental preliminary findings show that the yogurt in the EPS of the mouse thymus, spleen weight no significant impact. Phagocytic function and can activate mouse peritoneal macrophages and macrophage lysozyme activity; promote the generation of mouse serum hemolysin antibody, can improve the humoral immune function; enhanced mouse skin delayed-type hypersensitivity response, that can enhance the specificity of cellular immune function of the mice. The innovation of this study is extracted from the fermentation of yogurt in the EPS, and the extracted EPS immune activity research, provide direct experimental evidence for the yogurt with immune function. Yogurt producing bacteria of EPS production is increased by a method to improve the culture conditions, and based on this further development of lactic acid bacteria extracellular polysaccharide products, to be applied to drug production. Therefore, the issues are important to both the theory and practical application value.

Related Dissertations

  1. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  2. The Study on the Effect of the Transport Stress and Anti-stress Treatment on the Physiological Function of Beef Cattle,S858.23
  3. Effects and Some Mechanisms of TACI-Ig on Immune Function in MRL/lpr Mice,R593.241
  4. Soy Germ Isoflavone Conversion by Lactic Acid Bacteria and Development on Its Chewing Tablet,TS214.2
  5. Study on Enhancing Immune and Toxicology of Propolis Soft Capsule,R285
  6. The Processing Technology Studying of Fermented,TS251.65
  7. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  8. Effects of Postoperative Analgesia with Parecoxib Sodium and Fentanyl on the Immunity of Gynecological Patients,R614
  9. Breeding of the High Temperature Resistant High Production Lactobacillus Rhamnosus and Study of the New Lactic Acid Fermentation Technology,TQ921.3
  10. Research on the Effects of Different Routine Experimental Procedures and Rearing Environment on Wistar Rats,S865.12
  11. A Clinical Study on Jiangqi Hewei Tongfu Method to Promote Gastrointestinal and Immune Function for Postoperative patients with Intestinal Tumor,R273
  12. Effect of Radix Hedysari and Its Compound on Performance, Antioxidation and Immune Function of Broilers,S831.5
  13. Experimental Study of Cell Immune and Intestinal Barrier After Severe Trauma - Hemorrhagic Shock in Rats,R641
  14. Integrative Medicine Clinical study of chronic pyelonephritis,R692.7
  15. Clinical Immunity Study on Treating Endometriosis with the Method of Bushen Quyu,R271.9
  16. The Correlative Relationship between Human Parvovirus B19(HPV-B19) Infection and Idiopathic Thrombocytopenic Purpura(ITP),R725.5
  17. The Comparative Study Between Laparoscopic Splenectomy with Periesophagogastric Devascularization and Open Surgery on Immune System,R656
  18. Of Aureobasidium pullulans exopolysaccharide nitrogen source under accumulation and its application,R151
  19. Mutation Analysis the Genes of EMP Pathway in Xanthomonas Campestris pv. Campestris,S432.42
  20. Identification of Regulatory Genes of Three EPS and Pathogenicity Related Genes in XCC,S432.42
  21. Study on Synergetic Antibacteria Actions and Changes of Immune Function of TMP Combined with Chinese Herbs,S853.7

CLC: > Biological Sciences > Microbiology > Applied Microbiology > Immunology
© 2012 www.DissertationTopic.Net  Mobile