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Study on Cinnamomum Camphora Presl New Germplasm with Cold Resistance Through Genetic Engineering

Author: WangChangXian
Tutor: ZhaoLanYong;WangYuShan
School: Shandong Agricultural University
Course: Agricultural Extension
Keywords: Camphor Cold resistance gene AmGS Gene transformation PCR detection Resistance to the cold
CLC: S792.23
Type: Master's thesis
Year: 2009
Downloads: 68
Quote: 1
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Abstract


Camphor tree (Cinnamomum camphora Presl) , also known as camphor , evergreen foliage beautiful aroma rich , China 's southern city very beautiful and excellent street trees , landscape plants gardening landscape indispensable in the formation of one of the trees . Wintering in the north because of the cold in winter and it is hard to limit the cultivation of the species northward . The aim of this study through the Cold genetic transformation improve hardiness of camphor , to winter safely in Tai'an . The study by Agrobacterium-mediated transformation of camphor sand holly of Cold genes AmEBP1 and AmGS transformation, and the identification of molecular biology and the expression of cold hardiness trials , research findings are as follows : 1 , screening suitable incense camphor -induced differentiation and subculture proliferation the medium formula obtained camphor lateral buds of differentiation by NAA , 0.1mg/LNAA . lateral bud induction obvious effect of early differentiation . Leaves callus induction medium BA :2,4-D 1:4 , the highest rate of callus induction . Mitogen subculture BA for the best results , and the auxin IBA for the best . The best recipe for the proliferation of cultured MS 5.0mg/LBA 1.0mg/LIBA. 2 , the use of the newly constructed sand holly of Cold genes AmEBP1 and AmGS dip method using Agrobacterium , camphor Agrobacterium-mediated genetic transformation system , kanamycin resistant plants . PCR confirmed integration AmEBP1 , and AmGS gene the camphor genome resistant plants . 3 , the resistance to the cold test showed that camphor transgenic plantlets at -17 ℃, 2h conditions under mortality reduced by 67.0% , respectively, compared with the control ; as the temperature decreases , gene conversion of strains in the range of 0 ~ -16 ℃ Department of the gradual increase in the relative conductivity , cell membrane permeability and constantly enhance lethal temperature (LT50) was reduced 1.12 ° C , cold resistance enhancements .

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Camphor
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