Dissertation > Excellent graduate degree dissertation topics show

Neuroprotective Effects of Ginsenoside Rd and Isoflurane Preconditioning on Focal Cerebral Ischemia-reperfusion Injury in Rats

Author: LuYao
Tutor: XiongLiZe;LuZhiHong
School: Fourth Military Medical University
Course: Anesthesiology
Keywords: Cerebral ischemia Reperfusion injury Pretreatment Pretreatment Isoflurane Ginsenoside Rd
CLC: R743.3
Type: Master's thesis
Year: 2009
Downloads: 38
Quote: 0
Read: Download Dissertation

Abstract


The well-known acute cerebral infarction can cause varying degrees of neurological impairment, not only affect the patient's quality of life, severe cases can be life-threatening. Compared to non-ischemic preconditioning more difficult maneuverability and ischemic preconditioning in clinical clinical application and research value. Isoflurane as an inhalation anesthetic, has been widely used in clinical studies to prove its efficacy cerebral protective effects. Has confirmed the protective effect of the experimental model used almost all single ischemic preconditioning induced short and rapid ischemic tolerance. Long-term repeated isoflurane pretreatment whether cerebral ischemia reperfusion injury the role of superimposed enhanced protection, whether the strengthening of protection role has not been clearly demonstrated that the increase of pretreatment time. different monomer [2-5]. Has already identified structure of saponins monomer is at least 40 or more, but only ginsenoside-Rd few monomer has entered into a phase III clinical trials, can be used in clinical practice. The study was limited to this stage of ginsenoside monomer Rd protective effect after ischemia, no ginsenoside-Rd pretreatment cerebral protective effects reported. For perioperative cerebral ischemia, pre-administration undoubtedly more meaningful. This experiment using the rat middle cerebral artery occlusion (MCAO) model, both clinically feasible and convenient way to start a protective effect from long-term repeated isoflurane preconditioning and ginseng saponin-Rd pretreatment, explore the rat Bureau The protective effect of focal cerebral ischemia-reperfusion injury, better protection methods aimed at further ischemic brain injury. The first part of the repeat the isoflurane preconditioning purpose of a protective effect against focal cerebral ischemia-reperfusion injury. Study repeated isoflurane preconditioning on focal cerebral ischemia-reperfusion injury in rats 2. of COX-2 in repeated isoflurane pretreatment role in cerebral protection method repeated isoflurane preconditioning on focal cerebral ischemia-reperfusion injury 50 male SD rats (280 to 320g) 9 to 10 weeks old, were randomly divided into 5 groups (n = 10). The control group (CON): ischemia reperfusion group, the the ISO5d group the, ISO10d group, ISO15d group and ISO20d group. Same time every day inhalation one hour at the same concentration of isoflurane (1.5%) mixed gas and air, respectively continuous days. The 50 batch rats were randomly divided into a special the inhalation anesthetic preconditioning Box air intake through a gas mixture of air and ISO, another monitoring hole connected to the anesthetic gas analyzer for continuous monitoring box gas concentration. This device for my laboratory dedicated to the special case of animal pretreatment tank. Daily 1h isoflurane mixed with air and the gas pre-treatment, the animals in each group inhaled days. All rats I Division classic brain artery occlusion (MCAO) model. Respectively reperfusion 24h, 48h and 72h neurological behavior score the (neurologic behavior scores, NBS), and in the last score of neurological behavior after all rats were decapitated brain measuring infarct volume percentage. 2.COX-2 repeat the role of the isoflurane preconditioning brain protection in clean male SD rats were randomly divided into four groups (n = 5), the normal group (Normal), ISO10d the group, ISO20d group and control group (CON). All test rats after ischemia-reperfusion 24h with paraformaldehyde perfusion, the brain was made into frozen sections, immunohistochemistry. Repeated isoflurane preconditioning on focal cerebral ischemia-reperfusion injury in the protective effect ISO5d group the, ISO10d group, ISO15d group reperfusion 24h, 48h and 72h after neurological behavior score was significantly better than the control group (P lt; 0.05) between, ISO20d group and the control group, no significant difference (P gt; 0.05), ISO10d group of neurological behavior scores were significantly higher in each group (P lt; 0.05). Infarct volume percentage of the results, and then the percentage of infarct volume perfusion the 72h after ISO5d ISO10d and ISO15d group were (31.60 ± 9.00)%, (26.90 ± 8.26)% and (35.20 ± 5.37)%, and significantly less than the CON group (49.30 ± 6.00)% (P lt; 0.05) ISO20d group (51.50 ± 7.84)% CON group without statistically significant differences (P gt; 0.05), and the smallest to ISO10d infarct volume (P lt; 0.05). ISO10d group compared to ISO15d, and ISO20d group, the percentage of infarct volume was significantly increased (P lt; 0.05); compared ISO10d, and ISO5d group had no significant difference (P gt; 0.05). 2. COX-2 in repeated isoflurane preconditioning of COX-2 cells in brain protection the the CON group ISO20d group COX-2 cells positive immune response intensity significantly higher than the Normal group, the positive expression in the rat brain cortex results show that: and ISO10d group (P lt; 0.05), while Normal group and ISO10d group no significant difference was no statistical significance (P gt; 0.05); compared with CON ISO10d group of COX-2-positive cells apparent strength of the immune response weakened, and the number of positive cells was significantly reduced, and the difference was statistically significant (P lt; 0.05). Purpose of a protective effect of the second part of ginsenoside-Rd on focal cerebral ischemia-reperfusion injury. Research ginsenoside-Rd on cerebral ischemia-reperfusion injury protective effect of dose - response relationship of COX-2 ginsenoside-Rd cerebral protection method. ginsenoside-Rd cerebral ischemia reperfusion injury protective effect of dose - effect relationship healthy male Sprague-Dawley (SD) rats 70, 9 to 10 weeks , weight 280 ~ 320g (provided by the Experimental Animal Center of the Fourth Military Medical University). Randomly divided into 7 groups (n = 10), ischemia-reperfusion group (CON), Rd5mg/kg groups the, Rd10mg/kg group the, Rd20mg/kg group the, Rd40mg/kg group the, Rd80mg/kg group and propylene glycol group ( VEC group). The amount of the Rd group SD rats were injected intraperitoneally 1 hour before MCAO diluted to 6ml volume of different concentrations of ginsenoside-Rd. VEC group SD rats propylene glycol 6 ml of intraperitoneal injection of 1 hour before MCAO. 2.COX-2 ginsenoside-Rd cerebral protection of clean grade 20 male SD rats were randomly divided into four groups (n = 5), the normal group (Normal), Rd40mg/kg simple dose group ( Rd 40 'group), the CON group and Rd40mg/kg group of (Rd40 group). Rd 40 groups artery occlusion model of MCAO rats production by rat peritoneal single 1h give Rd40mg/kg Rd 40 'group without MCAO model rats, the only celiac single doses Rd40mg/kg time. All test rats after ischemia-reperfusion 24h with paraformaldehyde perfusion, the brain was made into frozen sections, immunohistochemistry. Results. Ginsenoside-Rd dose - response relationship of cerebral ischemia and reperfusion injury neurological behavior score Rd5mg, Rd10mg, Rd20mg, Rd40mg and Rd80mg group after I / R 24h, 48h and 72h NBS compared with CON group were significantly increased (P lt; 0.05), and the dose is less than 40mg/kg, increases with increasing dose Rd (P lt; 0.05). NBS of Rd80mg group to lower than Rd20mg and Rd40mg group (P lt; 0.05), compared with Rd5mg, Rd10mg two groups had no significant difference (P gt; 0.05). The VEC group and CON group had no significant difference (P gt; 0.05). Infarct volume percentage of the results, and then perfusion the 72h after Rd5mg Rd10mg Rd20mg Rd40mg and Rd80mg group infarct volume percentage were (34.80 ± 4.31)%, (29.60 ± 4.22)%, (24.70 ± 3.37)%, (17.50 ± 3.10)% and (31.60 ± 5.70)%, significantly less than the CON group (51.10 ± 6.60)% (P lt; 0.05), and minimum Rd40mg group infarct volume (P lt; 0.05). Compared with Rd40mg and Rd20mg group Rd80mg group, infarct volume percentage increased significantly (P lt; 0.05), compared with no statistical difference (P gt with Rd5mg, and Rd10mg group; 0.05). According to the experimental data to map out the Rd and infarct volume curve of the dose-effect relationship between the dose-effect relationship in between 5 -40mg/kg,. 2.COX-2 ginsenoside-Rd cerebral protection COX-2 cells results show positive expression in the rat brain cortex: the CON group COX-2 cells positive immune response intensity significantly higher than the Normal group Rd40 'group (P lt; 0.05), Normal group and Rd40 'group had no significant difference compared to no significant (P gt; 0.05); compared with the CON group the Rd40 group of COX-2 cells positive immune response intensity significantly weakened and the number of positive cells was significantly reduced, and the difference was statistically significant (P lt; 0.05). Conclusion 1. Repeated a certain concentration of ISO pretreatment cerebral protective effects of repeated 10-day protective effect for the best; pretreatment to 20 days, the protective effect but disappeared. Ginsenoside-Rd in advance administration can be significantly reduced in rats after focal cerebral ischemia-reperfusion injury, and a dose-response relationship, and to Rd40mg/kg as the optimal dose. ISO pretreatment and Ginsenoside-Rd is repeated cerebral protective effects may be related to the inhibition of COX-2 expression in the cerebral cortex.

Related Dissertations

  1. Assesment of Myocardial Protection of Isoflurane Versus Sevoflurane in Patients Undergoing Off-pump Coronary Artery Bypass Grafting,R614
  2. The Influence of Cold Self-bloodcardioplegia to MMP-2 Concentration for Infant with Cardiopulmulnary Bypass,R726.5
  3. Emulsified isoflurane on adult learning and memory function in rats,R965
  4. Remifentanil and isoflurane on liver function in patients with hepatocellular carcinoma Comparison,R614
  5. Domestic isoflurane inhalation anesthesia effect depends on learning and memory in adult rats related research,R965
  6. The Effect of Inhalation of Isoflurane on Learning and Memory Abilities and Hippocampal Synapse of Offspring Rats,R614
  7. Influences of Inhalant Anesthetics on Neuromuscular Blockade Produced by Cisatracurium in the Elderly Patients,R614
  8. Effect of Danhong Injection on Golgi Apparatus after Cerebral Ischemia Reperfusion in Rats,R285.5
  9. The Effect of Tetrahydroxystilbene Glucoside on the Protein Expression of HIF-1α and EPO in Cerebral Cortex of Old Rats after Cerebral Ischemia-reperfusion,R285.5
  10. Research on Anti-cerebral Ischemia Chemical Constituents from Ixeris Sonchifolia (Bge.) Hance, Quality Control and Their Passing Blood Brain Barrier,R285
  11. Study on Myocardial Energy Metabolism and Mitochondrial Function Changes in I/R,R363
  12. A Preliminary Study of Myocardial Protective Role of Nucleolin Using Transgenic Mice,R363
  13. The Effects of Heat Shock Pretreatment on Rat Myocardial Apoptosis and Expression of Metallothionein,R614
  14. Effects of Remote Ischemic Preconditioning and Remote Ischemic Postconditioning on Myocardial Injury in Adults Undergoing Valve Replacement Surgery,R654.2
  15. The Protective Effect of Splenectomy on the Hepatic Ischemia Reperfusion Injury,R657.3
  16. Ischemic Postconditioning Attenuates Lung Ischemic/Reperfusion Injury Induced by Cardiopulmonary Bypass,R654.2
  17. Mobilization of Bone Marrow Stem Cells by G-CSF Accelerates Renal Recovery after Ischemia-Reperfusion Injury in Mice,R692.5
  18. Effect of Repetitive Transcranial Magnetic Stimulation (rTMS) on the Hemorheology of Incelebral Infarction Rats,R743
  19. Experimental Study of the Chemotaxis Effect of Vegf in the Treatment of Cerebral Ischemia with Bone Marrow Stromal Cells,R743.3
  20. MK801 on the Expression of Bcl-2、Bax、C-fos Gene and Its Protective Effects to the Neurons in the Hippocampus CA1 Region of the Rats after the Brain Ischemic-Reperfussion Injury,R743.3
  21. The Study on the Effect of Exercise Training Intensity and ES on Recovery Mechanism and Function in Cerebral Stroke,R743.3

CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
© 2012 www.DissertationTopic.Net  Mobile