Dissertation > Excellent graduate degree dissertation topics show

Stimulation of γδT Cell in Peripheral Blood by Zoledronic Acid and Its Cytotoxic Effect in Nonspecific Immunity of Hepatoma.

Author: DaiZuo
Tutor: QianHaiZuo;LiuJianXia
School: Suzhou University
Course: Department of General Surgery
Keywords: γδT cells Zoledronic acid Proliferation Hepatoma cells Cytotoxic
CLC: R735.7
Type: Master's thesis
Year: 2007
Downloads: 39
Quote: 0
Read: Download Dissertation

Abstract


Part I: zoledronic acid stimulate human peripheral blood the γδT cell proliferation purpose : to explore zoledronic acid stimulation of human peripheral blood γδT cell proliferation role : collect peripheral blood of 10 healthy donors 10 ml of isolated from peripheral blood by density gradient centrifugation mononuclear cells. The use of third - generation bisphosphonate drug zoledronic phosphonic acid as a stimulating factor , zoledronic acid and IL-2 stimulated peripheral blood γδT cell proliferation in different concentrations of S - 3307 . The cells were cultured for 12-14 days , the cells were collected , flow cytometry related phenotypes , 3H - TdR incorporation determination of the degree of cell proliferation . Results: The cultured for 12-14 days , peripheral blood γδT cell proliferation significantly compared with the control group , the experimental group γδT phenotypic ratio increased significantly ( 9.7% of the control group and the experimental group 80.8 % , P lt ; 0.01) . CD3 increased significantly ( 71.2% of the control group , the experimental group, 96.2 % , P LT ; 0.01) . 3H-TdR incorporation assay cell proliferation significantly in the experimental group : (blank control group CMP value : the CMP value of 111.1 , the experimental group : 17488.6 P lt ; 0.01) . Zoledronic acid concentration in all azole In 1μΜ concentration , γδT cell proliferation most obvious . Conclusion : Zoledronic acid can promote human peripheral blood the γδT cell proliferation , a large number of high-purity γδT cells . Part II: amplified γδT cells : To investigate the purpose of human hepatoma cell killing effect to zoledronic acid to stimulate the proliferation of γδT cells of human hepatoma cell killing effect : the same experiment a method to obtain healthy human peripheral blood γδT cells , γδT cells as effector cells , respectively, to the human hepatoma cell line SMCC -7721 , MHCC- 97 , HepG2 as target cells . After the termination of the experiment were cultured for 48 hours in accordance with the effective target than 20:1,10:1 SRB assay killing efficiency of effector cells to target cells . : ΓδT cells of three tumor cells showed strong killing effect efficiency ratio of 20:1 , γδT cell destruction rate of liver cancer cells up to 98.7% , effector to target ratio of 10:1 , γδT cells of liver cancer cells The destruction rate of up to 92.6% . Same effector to target ratios , γδT cell killing effect of the different cell lines without significant differences ( P gt ; 0.05) different effector to target ratios , γδT cell killing effect of the same cell lines were significantly different ( P lt; 0.05 ) . Conclusion: γδT cells in vitro amplification zoledronic acid stimulation significant killing effect on liver cancer cells

Related Dissertations

  1. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  2. Proliferation Characteristics of Type 2 Porcine Circovirus Strains and Antivirus Activity of Iprs and Aps on PCV2 in Vitro,S858.28
  3. Antitumor Effects of Elemene on the Proliferation Inhibition and Apoptosis Induction in Ascites Hepatoma Cell Line H22,R735.7
  4. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  5. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  6. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  7. The Experimental Study of Semiconductor Laser Radiation and 5-FU Sustained Release Repressed Oral Malignant Tumor Cell,R739.8
  8. Urinary Inflammatory Myofibroblastic Tumor Clinicopathological Analysis and Literature Review,R737
  9. Study on the Cell Cycle of Liver Cells during Rat Liver Regeneration,Q253
  10. Effects of Different Immunization on the Development of Immune Organs,cell Proliferation and Expression of Apoptosis-relatived Gene in Chicken,S858.31
  11. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  12. Effect of Esculentoside a on Apoptosis and Expression of PCNA、Caspase-3、Fas and FasL in Renal Tissue of BXSB Mice,R285.5
  13. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  14. Down-regulation of ROCK Isoforms on Migration and Proliferation of Vascular Smooth Muscle Cells Stimulated by PDGF,R363
  15. Maximum Tolerated Dose Combined with Metronomic Chemoterpy on Proliferation and Angiogenesis in Treatment of Breast Cancer,R737.9
  16. Expression of rBTI-2 and Its Anti-tumor Activity,R730.2
  17. Effect of Ornithoctonus Huwena Venom on Proliferation of Human Colon Carcinoma Cell SW480 in Vitro,R735.35
  18. Gambogic Acid Blocks Proliferation and Induces Apoptosis of Lung Ddenocarcinom A549 Cells Through Downergulation of STAT3,R734.2
  19. Effects of NF-кB Signaling Pathways on Cyclic Stretch―Induced Human Periodontal Ligament Fibroblasts Proliferation and Its Mechanism,R781.4
  20. L-4- nitrobenzene alanine amino acid derivatives such as five kinds of biological effects,R96
  21. L-4- fluoro- phenylalanine derivative of 6 kinds of amino acids of the biological role of,R96

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile