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Positive Research on Clinical Characteristic of Coronary Heart Disease--Qi Deficiency and Blood Stasis and Its Curing Method--Activating Blood & Removing Blood Stasis

Author: MoLi
Tutor: YuanZhaoKai
School: Hunan University of Traditional Chinese Medicine
Course: Diagnostics of Chinese Medicine
Keywords: CHD Qi Deficiency and Blood stasis clinical features Activating blood & Removing blood stasis Angiogenesis Proliferation of cells The cell migrates culture The cell ready-made a tube
CLC: R259
Type: PhD thesis
Year: 2008
Downloads: 225
Quote: 0
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Abstract


Objective:This paper mainly research on the most common heart Qi Deficiency and Blood stasis symptom of coronary heart disease (CHD). Three syndromes of literature, clinical and experimental, and the clinical intervention mechanism of Activating Blood & Removing Blood Stasis, have offered basis for research to improve clinical differential diagnosis and treatment.Methods:1.Literature:Adopting methods of literature collection, collation, analysis and summary of, we carried out studies on the blood stasis for coronary heart disease Qi Syndrome standards, the objective of the model and measurement diagnosis, and etc.2.Clinical Research:(1) passes the investigation developing an epidemiology to coronary heart disease doctor of traditional Chinese medicine syndrome, method such as model and analysis applying the host be accomplished analysis, Logistic return, the certificate certificate type summing up coronary heart disease heart blood lacking in vital energy stagnating distributes a characteristic, and the clinical disease comparing the person with painstaking effort stagnating to hinder the certificate, the false ambition wane certificate collects the composition characteristic; The certificate relevance danger factor applying many factors of successive steps return analysis method stagnating to blood of coronary heart disease lacking in vital energy carries out analysis.(2) stagnates blood with the healthy people and the coronary heart disease gas stagnating for the certificate patient contrasting, from clinical experiment index (include complexion, tongue quality, A color and pulse condition and their microcosmic index), aspect stagnates to blood of coronary heart disease lacking in vital energy to verify the synchro collation being in progress observing analysis.3. Experimental study:(1) produced chicken chorioallantoic membrane (CAM) model, and take blank modules to the negative control group, basic fibroblast growth factor (bFGF) group and Shexiangbaoxin pill as a positive control group, make Continuous observation at a five time points, comparing drug CAM angiogenesis figure with intervention from different groups at the same time, and comparing CAM angiogenesis figure from the drug group and look at Yiqihuoxue Law Group’s Tablet and Yang Xin-Pulse whether there is a valid position to promote angiogenesis.(2) produced heart Qi blood stasis rat model using brominated diphenyl dimethyl thiazole tetrazolylazo (MTT) assay to detect the drug group on the cardiac blood stasis Qi rat vascular endothelial cell proliferation rate, NS group and blank serum as a negative control group, bFGF group and Shexiangbaoxin pill as a positive control group, observed by MTT assay role in rat vascular endothelial cells proliferation, and proliferation rate of between group, understanding Yiqihuoxue law Group’s YTⅠand YTⅡon vascular endothelial cell proliferation in vitro.(3) with scratches Experimental observation of the healing rate of endothelial cell migration, observing of the drug group scratches 0~24 hours after endothelial cell migration under inverted microscope and counting, compared to promote migration rate, to understand Yiqihuoxue law on endothelial cell migration affected.(4) making lumen count by detecting the formation of the lumen of vascular endothelial cells:observing under optical microscopy, connecting with endothelial cells that is a C-shaped lumen, low times in each glass microscope (×40) select three lumen intensive vision in each amplification (×100) lumen count the vision of a few. We obtain 10 images randomly from each group and calculating average value, comparing differences of each drug group, to understand Yiqihuoxue method’s influence on endothelial cells.Results: The first part:research literature on Qi blood stasis coronary heart disease Despite the heartache:(1)Chest is a combined effect of multiple factors, but Yang Yin-string is the key pathogenesis of coronary heart disease, blood stasis Qi Yin-Yang is the latest string interpretation of the development of coronary heart disease and the pathological basis.(2)Qi and blood stasis is a main type of coronary heart disease, its dialectical study of objective standards has certain development, Yiqihuoxue law is the treatment of Chest heartache Qi blood stasis.The second part:the clinical research of coronary heart disease and its characteristics——Qi blood stasis:(1)Qi blood stasis of non-coronary heart disease heart blood deficiency and heart-card portfolio simple, the main risk factors associated with age, smoking, body mass index, triglyceride, high-density lipoprotein cholesterol, such as plasma endothelin closely related.(2)Qi blood stasis and Qichixieyu card in the face, tongue, A, has its pulse on certain characteristics of the syndrome. Two patients of a certain proportion of blue, purple tongue, a stasis, pulse Shibuya levy, but Qi blood stasis patients face, tongue color, more TANSEI or whitish, thin and astringent chord pulse. The results showed that the microscopic detection, Qi blood stasis CHD patients than healthy control group, blood flow around the face resistance index (He/Hb) increased dynamic torsion plans to watch drag coefficient (h4/hl), cardiovascular, peripheral resistance (RT) were significantly increased microcirculation of red blood cell aggregation loop, loop top bleeding, slow-speed flow. CHD Qi blood stasis and Qichixieyu card, the card of the two micro indicators of the existence of a number of statistical significance (p<0.01 or p<0.05). Qi blood stasis with facial wave amplitude (Hb), rapid filling coefficient (Hb/Tab), the Pulse of the cardiac index ([t4-t1]/t), heart reactive coefficient (t1/t) and the cardiac function The four indicators Ce values (Sv, Co, Si, Ci) were significantly reduced its microcirculation of the loop short, fuzzy, vascular filling undesirable, such as hemochromatosis than desalination.The third part:Yiqihuoxue Qi blood stasis of promoting angiogenesis experimental study: (1)Yiqihuoxue law promoting chicken chorioallantoic membrane experimental angiogenesis:①0 hours and 24 hours, the drug group and the modules and gaps group was not significant (p>0.05) and the drug group no comparison group was significant (p>0.05).②48 hours, the drug group and the modules blank group are statistically significant (all p<0.05) between the drug group, compared with no statistical significance (p>0.05).③Every medicine has statistics meaning 72 hours, set without exception comparing with the model group, the blank space group (equal p<0.05); The nourishing heart exchanges the square I pulse group (YTI) and musk guarantees the group of heart ball group (SXBXW) there being no statistics significance comparatively (p>0.05); YT I has statistics meaning set comparing with bFGF group (p<0.05); The nourishing heart exchanges square II pulse group (YTⅡ) having statistics meaning comparing with SXBXW group (p <0.05); YTⅡhas no statistics significance set comparing with bFGF group (p> 0.05); YT I has statistics meaning set comparing with YTⅡgroup (p<0.05). Every urges the newborn number of blood vessel to form> the blank space group by forming> a model greatly till being bFGF group> YTⅡgroup> YT I group> SXBXW for a short time in proper order set. Every medicine has statistics meaning 96 hours, set without exception comparing with the model group, the blank space group (equal p<0.05); Every medicine group bears fruit comparatively with 72 hours.④every medicine urges the newborn effect of blood vessel to follow time fading away set but strengthens gradually. The model group and the blank space group count a blood vessel in five time though increasing by also step by step, the person increases but the group of extent without exception far inferior to every medicine. The YTⅡgroup and the bFGF group still keep an uptrend in queen return curve for 96 points invariable, two set of rooms have no statistics significance (p>0.05), but other every group queen return curve for 96 points memorial comes down trend.(2) benefit gases invigorate the circulation of blood following short rat blood vessel bast proliferation of cells experiment Every medicine has statistics meaning set without exception comparing with the normal saline group (NS group) and the blank space serum group (equal p<0.05), but NS has no statistics significance set comparing with blank space serum group (p> 0.05). Every medicine has the short rat blood vessel bast proliferation of cells effect set equally. Among them, YTⅡhas statistics meaning set without exception comparing with the YTI group, the SXBXW group (equal p<0.05), but bFGF has no statistics significance set comparing with YTⅡgroup (p>0.05). YTI has no comparatively also statistics significance between the group and the SXBXW group (p>0.05). Every urges the bast proliferation of cells effect reason to be greatly extremely for a short time in proper order set:Re-organize basicity becoming the fibre cell growth factor group (bFGF)> supports the throughⅡgroup (YTⅡ)> supports theⅠgroup (YTI)> musk guarantees the heart ball group (SXBXW)> normal saline group (NS)> blank space serum group (KX).(3) benefit gases invigorate the circulation of blood following short rat blood vessel bast cell removing an experimentEvery medicine has statistics meaning set without exception comparing with the NS group and the blank space serum group (equal p<0.05), but NS has no statistics significance set comparing with blank space serum group (p> 0.05). Every medicine is had set equally urging the rat blood vessel bast cell to remove an effect. Every medicine group bears fruit comparatively and proliferates an experiment. Every urges the bast cell to remove big extremely minor effect reason by:set BFGF forms> the YTⅡgroup> YTI group> SXBXW group> NS group> blank space serum group.(4) benefit gases invigorate the circulation of blood following short rat blood vessel bast cell Cheng being in charge of an experimentEvery medicine has statistics meaning set without exception comparing with the NS group and the blank space serum group (equal p<0.05), but NS has no statistics significance set comparing with blank space serum group (p> 0.05). Every medicine is had set equally urging the rat blood vessel bast cell to become the tube effect. Every medicine group bears fruit comparatively and proliferates an experiment. Every urges the bast cell to become big extremely minor tube effect reason By:set BFGF forms> the YTⅡgroup> YTI group> SXBXW group> NS group> blank space serum group. Conclusions:1. Qi Deficiency and Blood Stasis is the key to coronary heart disease pathogenesis, Qi Deficiency and Blood Stasis of CHD is the main type of TCM, Activating Blood & Removing Blood Stasisis is an important treatment method for coronary heart disease Qi blood stasis, which has solid theoretical, clinical and experimental basis.2. Qi Deficiency and Blood Stasis of CHD includes the symptom of qi deficiency and blood stasis, which reflect on the face, tongue and etc. This laboratory reaserch result have objective and micro indicators for clinical diagnosis and distinguishing of Qi deficiency and blood stasis of CHD.3. Coming into being invigorating the circulation of blood with benefit gas following cubic YTI and YTⅡbeing able to promote the newborn blood vessel of chicken embryo fine hair urine bag film model without exception, the law pointing out that the benefit gas invigorates the circulation of blood has urging the newborn effect of blood vessel.4. The benefit gas invigorates the circulation of blood following can urge the blood vessel freshman, prevention and cure coronary heart disease blood lacking in vital energy stagnates the certificate is that it can promote blood vessel bast proliferation of cells, migration and the cell ready-made a tube than the cell’s protection effect goes hand in hand, these protection effects show mainly to blood vessel bast.5. YTI of YTⅡbetter than composing in reply YTI though the effect is notable with being that method, but YTⅡurges the newborn effect of blood vessel and urges proliferation of cells, migration and ready-made tube benifit a gas invigorating the circulation of blood following set.

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