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Fabrication of Nanostructured ECM-Mimetic Interfaces for Control of Cell Adhesion Behaviors

Author: HuangJingHuan
Tutor: DingJianDong
School: Fudan University
Course: Polymer Chemistry and Physics
Keywords: The surface of the nano-patterning Nanoscale arrays Block copolymer micelles self-assembled template The regular degrees interference agent Technology transfer Imitation extracellular matrix RGD peptide ligand The spatial arrangement of the ligands Regularity Cell adhesion Focus adhesion Integrin protein PEG hydrogels. Viscoelastic Ligand critical spacing
CLC: TB383.1
Type: PhD thesis
Year: 2010
Downloads: 231
Quote: 1
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Abstract


Cell behavior is closely related to the chemical - mechanical characteristics of the base material. People are beginning to realize that the organization nano repair material surface characteristics determine one of the key factors acts such as cell adhesion and wound healing effect. In recent years, a variety of modified biological material to the specific cell adhesion ligands (such as arginine - glycine - aspartic acid tripeptide sequence, referred to as the RGD) continue to be developed for imitation extracellular matrix. Wherein the ligand containing the RGD sequence in the background material of the anti-cell adhesion spatial arrangement of the method to examine cells and extracellular matrix on a molecular level interaction provides an effective means. Research in this area there are still some very important issue worthy of our consideration, in particular spatial arrangement of ligand molecules Tacticity affect cell adhesion behavior \The purpose of this thesis work is to construct nano-patterning imitation extracellular matrix model, i.e. in the anti-cell adhesion of polyethylene glycol (PEG) on the substrate to build a cyclic Arg - Gly - Asp-D phenylalanyl acids - lysine [c (RGDfK)] polypeptide molecules composed of rules and irregular parallel control nanoarray used to examine the specific recognition of integrin protein (integrin) ligand molecules on the nanometer scale spatial arrangement For cell adhesion behavior. This is the first time the impact of the the RGD nanoarray Tacticity cells specific quantitative investigation. On the other hand, the preliminary investigation of the cellular response to the mechanical properties of the material body, designed the both hard nano-patterning and polymer substrates imitation extracellular matrix material system. The main innovation of the paper work to: (a) a rigid substrate surface using amphiphilic block copolymer micelles self-assembled template preparation rules and the method of the irregular parallel control array of gold nanoparticles. The Last expansion polymer micelle self-assembly template method (Add tacticity interference agent), to prepare a range of dimensions and tacticity adjustable array of gold nanoparticles in the surface of the inorganic material such as glass, silicon wafer. We investigated the impact of various experimental factors on gold nanoparticle arrays characteristic parameters (spacing, regularity, density, particle diameter) array of gold nanoparticles on the nanometer scale flexible regulation. In addition, the article also design new methods and software for statistical analysis of the morphology of the gold nanoparticle array. (B) based on above nano-pattern cell experiments found that the specific cell adhesion requires clustered distribution of the active ligand, the spacing of their adjacent molecules generally should be less than -70 nm. Portion of the focus of this paper is the preparation of nano-pattern of the cell adhesion contrast rigid substrate material surface and related cell adhesion behavior investigated. By anti-cell adhesion process on the surface of glass that graft polyethylene glycol silane reagent (PEG-Silane) and c (RGDfK) peptide molecules modified gold nanoparticle arrays prepared nano-patterning of cell adhesion contrast of a hard base material. C (RGDfK) polypeptide with Integrin Associated Protein-specific binding, so that the original arrangement of the array of the array of gold nanoparticles or polypeptide directly reflects the single cell focus focal adhesion composite structure of the adhesion (focal adhesion) arranged laterally of the integrin protein molecules way. Through the the parallel controlled investigated cell adhesion behavior, the experimental results show that the the peptide ligands average spacing greater than -70 nm, the regularity of the peptide array of cell adhesion has important implications. By the polypeptide nanoarray first parallel control irregular paper point spacing c (RGDfK) peptide sites average density and Bureau of parts successfully decoupled to verify peptide ligands local critical pitch (-70 nm) the presence of focal adhesion complex formation, above the critical spacing, the cell adhesion and severely suppressed. And found that the irregular polypeptide nano array, the lower the average density of the polypeptide locus can lead to stabilization and effective cell focus adhesion. Related cell biological mechanism is also described. (C) Preparation of PEG hydrogel surface nano-patterning and cell adhesion behavior research. In this paper, the latest expansion pattern transfer technology, with c (RGDfK) peptide molecular modification method combination, to prepared cell adhesion contrast the polypeptide nanoarray pattern in the PEG hydrogel material surface. PEG bulk mechanical properties of the hydrogel can be adjusted by changing the crosslink density of the polymer network. In this paper, frozen scanning electron microscopy characterization of a variety of nano-patterned surface morphology of the hydrogel material, and materials performance characterized. Preliminary interpretation of the experimental mechanism. On this basis, we preliminarily investigated the PEG hydrogel surface polypeptide nanoarray in morphology and the hardness of the material body of the cell adhesion behavior. In short, in this paper the development of nano-patterning imitation extracellular matrix material for the study of cell behavior provide innovative, practical research model and optimize the design of new nano-biomedical materials for the mechanism of interaction of cells with materials from the molecular level visits has positive significance.

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