Dissertation > Excellent graduate degree dissertation topics show

Experimental Studies and Clinical Significance of Bone Marrow Angiogenesis and Related Mechanism in Adult Patients with Acute Leukemia

Author: YeJin
Tutor: LinMaoFang
School: Zhejiang University
Course: Internal Medicine
Keywords: Leukemia, acute Angiogenesis , bone marrow VEGF, FLT-1, KDR, mononuclear cells , bone marrow VEGF supernatant bone marrow fluid Proliferation , ECV304 cells VEGF, K562 cells , and high Harringtonine alkali
CLC: R733.71
Type: PhD thesis
Year: 2002
Downloads: 95
Quote: 0
Read: Download Dissertation

Abstract


Neovascularization (angiogenesis), refers to the the neovascularization system generated from existing vascular bed. Involved in the degradation of extracellular matrix proteins, endothelial cell activation, proliferation, migration is a multi-step process. The process of angiogenesis by a variety of angiogenic factors, cytokines, and its inhibition factor between regulation. Vascular endothelial growth factor (vascular endothelial growth factor, VEGF) is the most studied, the strongest effect and specificity of the highest angiogenesis regulatory factor, specific pro-endothelial cell mitogen original. VEGF mainly its two tyrosine kinase receptors, fms-like tyrosine kinase (fins-like tyrosine kinase-1, FLT-1) containing the kinase domain the inserted fragments receptor (kinase-domain insert containing receptor, KDR) The specific binding of the to play an endothelial cell mitogen original bioactive. Studies have shown that many solid tumors, their growth, invasion and metastasis manifested as angiogenesis-dependent. Acute leukemia is a malignant clonal disease, levels of stem cells derived from hematopoietic system is a \(acute lymphoblastic leukemia, ALL) patients with bone marrow angiogenesis exists phenomenon. Recently foreign adult acute myeloid leukemia (acute myeloid leukemia, AML) bone marrow of patients with presence of angiogenesis There have been several reported in the literature, involving patients with acute lymphoblastic leukemia bone marrow angiogenesis only one, not yet reported. Adult patients with acute leukemia cells VEGF and its receptor FLT-1 and KDR gene expression levels, patient cells VEGF protein secretion levels between the different stage of disease and not of Zhejiang University Bo soil Dissertation same type of acute leukemia whether there are differences in the bone marrow of patients with liquid relationship between VEGF levels and bone marrow microvessel counts, both at home and abroad has not been elucidated. Whether the the homoharringtonine base the (HomoM towel Lantau ... nine, HHT) leukemia cells VEGF expression can decline, thereby affecting angiogenesis reaction play versus-leukemia effect? Has not been reported. Of 95 cases of acute leukemia patients (including onset untreated 70 cases, five cases relapsed or refractory, and bone marrow to ease 20 patients) and 15 normal adult bone marrow biopsy specimens for study. Immunohistochemical method using polyclonal antibody to rabbit anti-human coagulation factors in the dish associated antigen (Fattof h-RClatCd Afitigap VOfl illCbfalld Factofg vWF) as a marker of endothelial cells under an optical microscope (X 400, each field of view on behalf of area 0 .233 nun Diao counts bone marrow biopsy microvessel counts (the average number of individual vision microvascular microvessel counts) as the film's results show that the onset of the untreated group of bone marrow microvessel counts (23.09 ± 3.741 X400 vision) in the control group (7.41 the disabilities l.49/X44 vision) and bone marrow remission group (8.40 ± l.20 / x400 field) <0.001), the number is still higher than the bone marrow remission bone marrow microvascular normal control group, the difference was statistically significant (pod.05); bone marrow microvessel counts in untreated group with relapsed or refractory groups showed no significant difference (p> 0.05). In untreated group, the microvessel counts in 23 patients with ALL and 43 cases of acute non-lymphocytic leukemia (leukemia of acute nonlpophoblastic, the difference between the patients with ANLL), differences not statistically significant (23.90 ± 4.14 / x400 vision the vs 22 stone 1 ± 3 1/X400 vision, pod.05); FAB subtypes Ml. And i. Bone marrow microvessel counts between patients were not significantly different (22.89 the soil 3.50/X400 vision VS22 disabilities 2.96/X400 vision, p> 0.05); bone marrow microvessel counts and bone marrow blasts percentage was a certain correlation (r - AI.311, the plant knife 09); Microvessel counts at diagnosis were bone marrow remission after treatment differences between patients with and without bone marrow remission patients was not statistically significant (p kiss .05 ). The results show that in patients with acute leukemia bone marrow microvessel increase in the number exists. Prognostic indicator of ability to obtain bone marrow remission in newly diagnosed patients with bone marrow microvessel counts than induction therapy. To investigate the possible role of the regulation of angiogenesis factor VEGF in the pathogenesis of acute leukemia patients, we use the RT-PCR, bone marrow mononuclear cells cultured in vitro ELISA detection and MTT colorimetric method: on a different stage of disease and the different types of acute leukemia bone marrow mononuclear cells (MNCS) of VEGFmRNA Zhejiang University Bo soil thesis and its receptor the FLT-IInRNA and KDRmRNA expression levels of bone marrow fluid and bone marrow MNCs culture supernatant levels of VEGF, bone marrow MNCs culture supernatant on human umbilical vein endothelial cell line ECV304 cell proliferation, as well as the relationship between the number of bone marrow fluid content of VEGF and microvessel. In this study, using RT. PCR method detected 69 cases of acute leukemia patients and 16 normal controls bone marrow MNCsVEGFmRNA expression. The results show that 48/49 cases of acute leukemia in untreated patients, 3/3 patients relapsed patients, 16/17 patients with bone marrow remission and 13/16 normal controls bone marrow MNCs

Related Dissertations

  1. DNA-Dependent Protein Kinase Catalylic Subunit in Adult Acute Leukemia,R733.7
  2. Expression of Axl and Its Ligand Gas6 in Acute Leukemia and Their Clinical Significances,R733.71
  3. Detection and Clinical Characteristics of MLL Gene Rearrangement and FLT3/ITD in Acute Leukemia,R733.7
  4. MDM2 SNP309 polymorphism in acute myeloid leukemia and the role of risk sensitivity to chemotherapeutic drugs,R733.71
  5. The expression of the of Midkine gene in acute leukemia and leukemia cell proliferation activity,R733.7
  6. Clinical and Laboratory Studies of Childhood Acute Leukemia,R733.7
  7. The Expermental and Clinical Study on Apoptosis Factors and multidrug Resistance in Acute Leukemia,R733.71
  8. The Basic and Clinical Study of Notch Signaling in the Acute Leukemogenesis,R733.71
  9. Acute leukemia cytogenetics and epigenetic research,R733.7
  10. Expression of Telomere Binding Factor 2 (TRF2) on Leukemia Cell Lines and Primary Leukemia Cells,R733.7
  11. Study of NF-κB in the Regulation of Multidrug Resistance Gene Transcription in Acute Myeloid Leukemia,R733.71
  12. Expression and Significance of Cyclooxygenase-2 in Bone Marrow Cells of Acute Leukemia Patients,R733.71
  13. Patients with acute leukemia vascular endothelial growth factor and basic fibroblast growth factor and its clinical significance,R733.71
  14. Investigation of Expression of Vascular Endothelial Growth Factor in Acute Leukemic Cells,R733.71
  15. CXCR4 expression in acute leukemia and its clinical significance,R733.71
  16. The Role of 4-1BBL Reverse Signaling on the Biofunctions of M5 Leukemia Cells and the Underlying Mechanisms,R733.7
  17. A Related Study of PRAME Gene Expression in Adult Acute Leukemia,R733.71
  18. Serum Levels of Basic Fibroblast Growth Factor and Tumor Supplide Group Factors and Their Clinical Significance in Patients with Acute Leukemia,R733.71
  19. Expression and Clinical Significance of Vascular Endothelial Growth Factor in Patients with Acute Leukemia,R733.71
  20. The Study of Molecular Genetics of Mutation of PTEN Gene in Acute Leukemia,R733.7

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
© 2012 www.DissertationTopic.Net  Mobile