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Studies on Solubilizing Effects on Phosphate Rock Powder and Insoluble Phosphorus in Soil of P-solubilizing Microorganisms and Their Mechanism

Author: ZhongChuanQing
Tutor: HuangWeiYi
School: Nanjing Agricultural College
Course: Microbiology
Keywords: Solubilizing microorganisms Bacillus megaterium Organic acids Acid Phosphatase Alkaline phosphatase
CLC: S154.3
Type: PhD thesis
Year: 2004
Downloads: 1681
Quote: 16
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Executive Summary


Separation from solubilizing microorganisms, screening starting over two hundred strains solubilizing microorganisms isolated from multiple soil samples from all over the country and the seed coat, after initial screening, rescreening, finally got the better solubilizing microorganisms strains, including bacteria, yeasts and molds, parts of China solubilizing microorganisms resource preliminary investigation. By colony, cell morphology, the propagation of judgment as well as physiological and biochemical assay, preliminary solubilizing microorganisms P17 strain, P10 strain Y3 strain F4 strains were isolated and identified as Bacillus megaterium (Bacillus megaterium), Brevibacterium (of Brevibacterium) , Rhodotorula (Rhodotorula) and specifically spp, (Penicillium). The different effects of different solubilizing microorganisms dissolved phosphorus. From the test results, Ca 3 (PO 4 ) 2 , FePO 4 , AlPO 4 and other insoluble phosphate easily dissolve yeast, mold and rock phosphate Bacillus dissolved variety of different dissolving microorganism on pro-phosphorus-containing substances of different chemical structures and the ability to dissolve. In this research, the four solubilizing microorganisms has good representative, is a production of Bacillus, Brevibacterium, yeasts and molds. The study showed that the mold is not the ability to dissolve all forms insoluble phosphorus are higher than bacteria, bacteria, yeast, etc. in the dissolved phosphorus also plays an important role. Ghani A (1994) and Kucey (1983) reported that the fungal phosphate solubilization capacity than bacteria, this argument, there is a certain one-sidedness. Various microorganisms of different insoluble phosphates from the point of view of this study, the effect of different dissolved. Fungal growth, breeding need the carbon source, high energy, long growth cycle, but dissolve insoluble phosphate plays a large biomass, metabolites advantage; the bacterial P17 strains Rock Phosphate Solubilization ability, and on behalf of When short. The biological phosphorus fertilizer should be considered by the mixed flora of bacteria and fungi. If different kinds can complement each other in the to dissolve insoluble phosphate and rock phosphate solubilizing microorganisms mixed flora as biological phosphate fertilizer, will make a greater contribution to agriculture. Solubilizing microbial phosphate solubilization effect studies have shown that the P17 strain was inoculated from different sources of rock phosphate increased available phosphorus content of the fermentation broth is the only source of phosphorus fermentation broth. Visible the P17 strain solubilizing microorganisms can be effectively dissolved transforming insoluble phosphorus in the phosphate rock, it is feasible to improve rock phosphate applied directly effect Microbiology pathway. Yunnan Haikou phosphate rock, Kaiyang Phosphate, Hubei Yichang phosphate sedimentary phosphate rock gold card yellow phosphate rock, Hubei Huangmailing Phosphate was the metamorphic phosphate rock phosphate rock geological formation conditions, phosphorus available phosphorus content of the slag are very different. P17 strains were inoculated Huangmailing yellow phosphorus ore and gold card phosphate solubilization largest increase easily solubilizing strains of microorganisms into metamorphic type of phosphate rock phosphorus, dissolved. Flask test, scanning electron microscopy of biological weathering of the different sources of rock phosphate solubilizing microorganisms evidence elect P17 strains of Bacillus megaterium: the most suitable for the role of the type of rock phosphate. As can be seen from the shake flask test results, the P17 better ability to dissolve strains of metamorphic rock phosphate from the Huangmailing gold yellow card. Insoluble rock phosphate phosphorus gradually P17 strains dissolved down after the P17 strains up to 70d dissolution,. Huangmailing gold card yellow phosphorus slag after P17 strains up to 70d dissolved total available phosphorus content by the first inoculation fermentation the solution 291.46mg L -1 316.6mz L -1 increase the final 869.71mg L -1 837.04 mg L -1 , rock phosphate insoluble phosphorus gradually P17 strains dissolved down . Tenth incubation 81.02%, of rock phosphate Huangmailing phosphorus is dissolved down, then live bacteria filtrate cumulative available phosphorus is 5.93 times that of the control, then inactivated bacteria is 2.54 times that of the control. For gold card yellow phosphorus slag, P17 strain dissolved 78.97% of the total phosphorus, then live bacteria filtrate cumulative available phosphorus is 5.4 times that of the control, then inactivated bacteria was 2.32. Then inactivated bacteria into metabolites such as the difficult volatile Nanjing Agricultural University Doctoral Dissertation: solubilizing characteristics of microbial dissolution of rock phosphate and soil insoluble phosphorus and dissolved phosphorus ways Studies acid rock phosphate, so filtrate available phosphorus content increased. Rock phosphate insoluble phosphorus is mainly inorganic phosphorus. Belongs metamorphic phosphate rock the rock phosphate Huangmailing and gold card yellow phosphorus slag suitable for huge spores subtilis the P17 strain growth and effective metabolites generated, and thus prone to erosion. After the long-term effects of the P17 strain, most converted to phosphorus. To further confirm the above results, rock phosphate, a scanning electron microscope. Continue inoculated, inoculated, then inactivated bacteria were no significant differences among treatments plus blank medium, still a small part of the phosphorus in rock phosphate can not be the huge bud gowns bacillus P17 strains dissolved down that solubilizing microorganisms only impossible all the phosphorus from rock phosphate dissolved dissolved phosphate rock, within certain limits. Superphosphate is made of water-soluble phosphate fertilizer rock phosphate with sulfuric acid reaction. Its main component is a calcium phosphate [Ca (HZPo ;):. H20] and insoluble calcium sulfate. Applied into superphosphate, the slight decline in soil pH. With time, the pH of a downward trend increases. Superphosphate will expand with the free metal ions in the soil, such as c ', Fes, A13 decile combine to form insoluble phosphate deposition, trying to through the inoculated microbes in the soil to improve superphosphate immobilized soil phosphorus nutrition. The study results showed that the inoculation treatment process and not inoculated pH value smaller difference, no positive correlation with the increase in available phosphorus content. Insoluble phosphorus into available phosphorus, pH lowering is not the only factor. Add water, control soil samples pH changes. In addition to adding water control, soil samples of various treatments, such as inoculation, then inactivated bacteria, plus blank medium available phosphorus content is gradually increased. Plus superphosphate each inoculated processing soil samples, 10 Diao before non-sterile soil samples available phosphorus than sterilized soil, 10 Diao former than the latter, applying superphosphate treatment early indigenous microbial flora dominated, 10 Diao the P17 strains play a major role in all the results that have an important role in dissolving microorganism on the conversion of the yellow brown labile. Inoculation, then inactivated bacteria, add water medium for phosphatase activity were higher than the control; inoculated treatment phosphatase activity higher than pick inactivated bacteria plus medium processing; then inactivated bacteria medium between no significant difference. Sample in a pre-sterilized soil phosphatase activity is not high, with the time, solubilizing microorganisms increase in the number of phosphatase activity increased. Phosphatase for the induction of the enzyme, not only?

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CLC: > Agricultural Sciences > Agriculture as the foundation of science > Soil > Soil Biology > Soil microbiology
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