Dissertation > Excellent graduate degree dissertation topics show

Characteristics of Enzymes Involved in the Phenanthrene Degradation by Moderately Halophilic Martelella Sp. AD-3

Author: DongZuo
Tutor: FengYaoYu
School: East China University of Science and Technology
Course: Biochemical Engineering
Keywords: Polycyclic aromatic hydroxycarbons phenanthrene Martelella sp.AD-3 salicylate-5-hydroxylase dioxygenase
CLC: X172
Type: Master's thesis
Year: 2012
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


Salicylate5-hydroxylase and dioxygenase play important parts in the polycyclic aromatic hydroxycarbons (PAHs) degradation, the researches on the two enzymes in non-halophilic microorganisms, more rarely reported in the salt-tolerant and halophilic microorganisms. for environmental remediation is not applicable especially in the high-salt environment. The reseacheres are more concerned about salicylic5-hydroxylase in the role of the single ring and bicyclic aromatic compouds degradation, the less role of research in the degradation of tricyclic aromatic ones The paper was aimed to study the characteristics of phenanthrene degradation, the activities of key enzymes in the degradation, the optimal conditions of salicylic5-hydroxylase and the sequence features of PAHs dioxygenase gene in aerobic, moderately halophilic Martelella sp. AD-3.Phenanthrene could be removed under salinities from0.5%-15%. and removed completely under salinity3%,the concentration of phenanthrene200mg/L within6days in AD-3by HPLC3. Activity analysises showed efficient activation of1-hydroxy-2-naphthoic acid hydroxylase.1-naphthol hydroxylase. salicylic5-hydroxylase involved in the degradation of phenanthrene by strain AD-3. Salicylate5-hydroxylase was found to be most active.132.8nmol/min/mg. under the concentration of phenanthrene200mg/L. salinity3%and pH9.0. A length of592bp fragment, dioxygenase gene phn592, was cloned by semi-nested polymerase chain reaction, showed95%identity to the naphthalene1.2-dioxygenase gene from halophilc Marinobacter sp. NCE312by blast.The paper showed that the salinity of growth was broad, the rate of phenanthrene degradation was high in AD-3:the activity of salicylic5-hydroxylase was found to be positive correlation with the rate of phenanthrene degradation:the molecular machanisms were different in phenanthrene degradation between strain AD-3and other non-halophilic bacterium. The study provided an important reference for further characteristics of enzymes in PAHs degradation in moderately halophilic bacterium.

Related Dissertations

  1. Screening and Kinetics of High Strength Phenol Degrading Bacterial Strains,X172
  2. Comparative Analysis of mRNA Expression of Tumor Necrosis Factor Like Ligand 1A and Indoleamine-2, 3-dioxygenase in the Peripheral Blood Monocytes of the Patients with Rheumatoid Arthritis,R593.22
  3. Synthesis, Characterization and Properties of Phenanthrene Derivatives and Their Complexes of Iridium and Europium,O627
  4. The Expression and Significance of IDOmRNA in Peripheral Blood in Hazak’s Esophageal Cancer,R735.1
  5. Study on the Expression of IDO in MDSCs Derived from the Spleen of Lewis Lung Carcinoma Bearing Mice,R734.2
  6. Effects of Phenanthrene,benzo[b]fluoranthene on Perna Viridis,lutjanus Erythropterus and Lutjanus Argentimaculatus,X174
  7. Study on the Synthesis of New Efficient Herbicide Mesotrion,TQ457.2
  8. The Agrobacterium-mediated Transformation of Indica Rice and Molecular Basis of Jasmonate-induced Leaf Senescence,S511.21
  9. Colored cotton pigment genes GHLDOX gene function studies and colored cotton regeneration system,S562
  10. The Toxicity of Phenanthrene and Sodium Percolorate to Zebrafish,X174
  11. Studies on Interactions of Lou Soil - PAHs - Surfactant,X53
  12. Biodegradation of Phenanthrene by a Halophilic Bacterial Consortium and Expression of Naphthalene Dioxygenase Genes,X172
  13. The Experimental Research of Treatment for Transpanted Liver Neoplasm in Rats by Transcatheter Arterial Chemoembolization Combined with 1-methyl-tryptophan,R735.7
  14. Bacterial Consortium in the Biodegradation of Polycyclic Aromatic Hydrocarbons-Phenanthrene,X50
  15. Biodegradation of Di-n-butyl Phthalate and Dynamics Analysis of the Key Bacterial Community in Soils,S154.3
  16. Isolation and Identification of Aniline-degrading Strain and Preliminary Study of Degradation Characteristics,X172
  17. Isolation, Identification and Characteristics of Pyrene- and Fluoranthene-degrading Bacteria Isolated from Petroleum Contaminated Soil,X172
  18. A Study on the Extraction Rate, Bioavailability and Fraction Distribution of PAH_s at Different Aging Time,X53
  19. The Study on Effect of Nitrogen Forms and Light Intensity on Cucumber Seedling under Phenanthrene Stress,S642.2
  20. Heme Oxygenase Involved in Gibberellic Acid-Induced α-Amylase Expression and Delays Gibberellic Acid-Induced Programmed Cell Death in Wheat Aleurone Layer,S512.1
  21. Design, Synthesis and Herbicidal Activities of Cyclohexenone Derivatives,TQ457.2

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
© 2012 www.DissertationTopic.Net  Mobile