Dissertation > Excellent graduate degree dissertation topics show

Sequence Analysis to Mitochondrial12S rRNA Gene and Molecular Identification to Parasitic Mites in Mussels

Author: GuoWei
Tutor: WenChunGen
School: Nanchang University
Course: Hydrobiology
Keywords: Unionicolid mites 12S rRNA gene larvaes nymphan cryptic species
CLC: Q969.91
Type: Master's thesis
Year: 2012
Downloads: 29
Quote: 0
Read: Download Dissertation

Abstract


Water mites belong to one genus, Unionicola only, are common parasites of freshwater mussels. It is questionable subgeneric classification is based on characters of the adult, but comparative to characters of larvae, could accurate to determinant the phylogenetic position of adult, the Larvaes of water mite are small, Subgeneric classification is very difficult to rely upon morphological characters. Here, a portion of the mitochondrial12S rRNA gene from egg of water mite which was parasitic on Cristaria plicata was amplified by molecular biological technology, sequenced and comparative sequence analysis with mite water of GenBank. Identifying the species of eggs, describing the larvae and nymphan hatched from eggs. The result showed that the nucleotide homology of the mitochondrial12S rRNA gene of egg compared with Unionicola sp. was94.8%, the genetic distances was the lowerst, belonged to interspecific genetic differences. The eggs were the subgeneration of Unionicola sp. Comparative Parasitic water mite. Unionicola sp. and U. arcuata, the genetic differences of12S rRNA gene and morphological characteristics, the results of this study was suggested that Unionicola sp. was a cryptic species of U.arcuata.On the other hand,12S rRNA gene complete sequences of U. brevipedalis sp. which were parasites in Cristaria plicata was amplified, sequenced and analyzed. To acquire12S rRNA gene complete sequences of U. brevipedalis sp.648bp, the nucleotide distances with other mite water was0.987(with U. chelata)~1.073(with U. formosa), gnerally higher. Molecular tree showed that U. brevipedalis sp. formed one clade alone, closed to U. imamurai, acording to the Special morphological characteristics of acetabular plates, we inferred that U. brevipedalis sp. was the relatively ancient water mite.

Related Dissertations

  1. Sequences Variation of Mitochondrial 12S rRNA Gene and Molecular Phylogeny of 20 Species Birds(AVES: Charadriiformes),Q958
  2. System Analysis of Commom Cheletids (Acari: Cheyletidae) Based on Mitochondrial COⅠ and 12SrRNA Gene,S379.5
  3. Two kinds of competition between invasive whitefly replace its behavioral mechanisms,S433
  4. Effect of Vector Density, Plant Age and Temperature on the Transmission of Tomato Leaf Curl Taiwan Virus by the Whitefly Bemisia Tabaci,S433
  5. Molecular Phylogenetic Analysis of Some Species of Megaselia of China,Q961
  6. The Molecular Phylogenetic Research of Ips Typographus Based on Partial Sequences of mtDNA-12S rRNA, COⅠ, COⅡ Genes,S791
  7. Studies on Cladistic Systemics and EST Isozymes of Unionicolid Mites,Q959
  8. Research to Distinguish Bos Grunniens、Bos Taurus and Bubalus from Meat Products Mixture Based on Mitochondrion 12S rRNA Gene,S823
  9. Comparative Analysis on Some Genes of Mitochondrial DNA and Their Molecular Identification Markers in Three Species of Soft-turtles,Q78
  10. Studies on Genetic Structure and Phylogeny of Parasitic Unionicolid Mites (Acari: Unionicolidae: Unionicola) Based on Mitochondrial 12S rRNA Gene,S944.1
  11. The Symbionic Relationship of Several Psocid Species and Their Endosymbionts,S379.5
  12. Sequences Variation of Mitochondrial 12S rRNA Gene and Cytochrome b Gene and Molecular Systematic Studies of Anseriformes,Q953
  13. The Early History of Modern Birds and the Phylogenetic Study of Buttonquails, and Evolution of Microsatellite Alleles in Some Species of Heron (Family Ardeidae),Q951
  14. The Phylogeny of Genus Scylla and Population Genetic Structure of Mud Crabs Along Coast of China,S917.4
  15. Research on Molecular Phylogeography of Three Rhinolophidae Bats,Q953
  16. Studies on Systematics of the Family Ranidae in China,Q951
  17. Cryptic Diversity of Giant Spiny Frogs Inferred from Mitochondrial DNA Markers,Q958
  18. DNA Taxonomy in Marine Bivalves: DNA Barcoding in Mytilidae and Ostreidae, and Cryptic Diversity in the Pen Shell Atrina Pectinata,S917.4
  19. Analysis of Mitochondrial Gene and Feeding Habit of the Dominant Species and Molecular Classification of the Larvae in the Genus Unionicola(Acari:Unionicolidae)in China,Q963
  20. Divergence and Comparative Phylogeography of Ficus Pumila and Its Pollinating Wasps(Wiebesia Spp.),S186

CLC: > Biological Sciences > Entomology > Applied Entomology (Economic Entomology ) > Ticks and mites SCIENCE
© 2012 www.DissertationTopic.Net  Mobile