Dissertation > Excellent graduate degree dissertation topics show

Identification and Screening of Strain Transforming Rare Ginseng Saponins and Optimization of Transforming Conditions

Author: LiChengLong
Tutor: YuLei
School: Jilin Agricultural University
Course: Food processing and security
Keywords: Rare Ginsenoside Strain Screening Biotransformation Strain Identification Ginsenoside Rb1 Ginsenoside Rd
CLC: TQ461
Type: Master's thesis
Year: 2012
Downloads: 26
Quote: 0
Read: Download Dissertation

Abstract


Ginseng, the root of Panax ginseng C. A. Meyer, Araliaceae, is a well-known medicinal plant. It has been used as atraditional natural medicine in China, Japan and Korea, for thousands of years. Its chemical properties and pharmaceutical functions, such as anti-aging, anti-inflammation, anti-oxidation and so on, have been intensively studied throughout the world. Gingenosides are the main pharmacological active component of ginseng. Up to now, more than40ginsenosides have been isolated and characterized from ginseng roots, which including with the major ginsenosides Rb1, Rb2, Re, Rg1and Re that constituting more than90%of the total ginsenosides. The rare ginseng sapoins’pharmaceutical functions have been concerned by many people. And their get way also have been concerned by many people.The main purpose of this paper was to screen the strains that can carry out rare ginseng saponins transformation from TongHua forest soil and optimize the transforming conditions. The strains with the highest transformation activity were anticipated which was significant for the effective usage of ginseng and devoted to medical cause.Firstly, ginseng total saponins were separated from ginseng by using ethanol distillation and further glycol type ginseng saponins mixture by the column chromatography which including ginsengside Rb1、Rb2、Rc、Rd. The mixture was used as the substrate.Secondly,92strains were screened from the soil. The primary screening result showed that four strains can transform ginsenoside Rb1to ginsenoside Rd. The stain TH-10had the strongest transformation activity among them,The conditions of HPLC were studied for getting a more accurately qualitative and quantitative analysis result. The best condition of HPLC was attained as follows: chromatographic column:Ascentis Express C18(5cm×3.0mm,2.7μm), Mobile phase water(A)、acetonitrile(B), Column temperature:35℃, wavelength:203nm,1.0ml/min,5μl.Thirdly, the strain TH-10was identified belonging to Aspergillus, through the observation of the culture on plate, microscopic characteristics and molecular biology method.Finally, the study optimized the best conditions of inoculum age, temperature, pH and substrate concentration. The result showed that the highest conversion rate was73.77%with one factor a time method, when the inoculum age, temperature, pH and substrate concentration were48h,5,27℃and0.15mg/ml,respectively.

Related Dissertations

  1. Separation and purification of stevioside directional conversion and the product of sweet tea glycosides,S566.9
  2. Transformation of Specific Pollutants in the Process of Biotreatment of Acrylonitrile Wastewater,X783
  3. The Purification and Structure Modification of Ginsenoside-RB1,TQ461
  4. Biotransformation of Ginsenosides from Stems and Leaves by Plant Pathogenic Fungi,S567.51
  5. Biotransformation of Protopanaxadiol-type Saponins by a Kind of Oospora Wallr.,S567.51
  6. Study of Bioaccumulation, Distribution and Biotransformation of BDE209 in Mudsnail,X174
  7. Screening and Study of Riboflavin Biosynthesis Inhibitor Producing Strain,TQ460.1
  8. Intestinal flora of transforming the main component of licorice and efficacy,R284
  9. Producing strains of Burkholderia WGB30 anisaldehyde Identification and analysis of some metabolites,Q93
  10. Metabolic Engineering of Gluconobacter Oxydans 621H on Glucose Utilization and Its Biotransformation Study,Q935
  11. Study on α-ketoisocaproate Biosynthesis by Rhodococcus Opacus DSM 43250,TQ921
  12. The Study on the Extraction Method of Biotransformation and Cosmetic Efficacy of Oat β-glucan,TQ652
  13. Study on Production β-Alanine by Biotransformation,TQ922.2
  14. Isolation and Identification of Nitrile-Hydrolyzing Microorganisms and Biotransformation of β-Aminopropionitrile to β-Alanine Production by Stenotrophomonas Maltophilia a39,TQ922
  15. Preparation and Activity of Phenolic Compounds by Biotransformation of Rosin,TQ351.1
  16. Screening for Nitrilase-Producing Microbes and Biotransformation of P-Hydroxybenzyl Cyanide by Bacillus Subtilis E9,TQ925
  17. Gene Cloning and Expression of a Novel (S)- Carbonyl Reductase from Candida Parapsilosis,TQ925
  18. The Diversity of Ginseng Rhizosphere Soil Fungi and Endophytes and Screening of Ginsenoside Biotransformating Strains,Q93
  19. Preparation of Active Sesquiterpene Lactone Glucosides from Ixeris Sonchifia (Bge.) Hance and Studies on Their Biotransformation,TQ464
  20. Studies on Two Medicinal Sesquiterpene Lactones,R91
  21. Biotransformation of Rb1, Rb2 and Rc by Alternaria Porri (Ellis) Cifferri,S567.51

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > The Chinese herbal medicine preparations of the production
© 2012 www.DissertationTopic.Net  Mobile