Dissertation > Excellent graduate degree dissertation topics show

Study on the MPC(2-methacryloyloxy Ethyl Phosphorylcholine) Modified Single-walled Carbon Nanotubes and Its Bioeffects

Author: ZhaoZuoFeng
Tutor: Zhang
School: Nanjing University
Course: Materials Physics and Chemistry
Keywords: Modified Single-walled Carbon nanotubes Cytotoxicity Cell uptakemechanism
CLC: R318
Type: Master's thesis
Year: 2013
Downloads: 34
Quote: 0
Read: Download Dissertation

Abstract


During the2decades since the found of CNTs, it has been widely and deeply researched in relative areas because of its unique nano-structure and the unusual physicochemical, mechanical, and electrical properties. Subsequently, the greater possibilities for its potential application in biological and biomedical use were realized, which appealed a lot of researchers and soon became a focus of research. However, before CNTs could be really used in this area and fully exploited for commercial interests, it is an urgent issue to clarify the cytotoxicity of the CNTsThere is another concern well before the consideration of the CNTs’cytotoxicity, namely, the water solubility of the CNTs. Up till now, many methods have been proposed to solve this problem, the most effective way among them is using physical and chemical modification and functionalization.Given the premis above, the research in this article choosed2-methacryloyloxy ethyl phosphorylcholine (MPC) to modify Single-walled Carbon Nanotubes (SWCNTs) covalently to synthesis two kinds of water soluble modified SWCNTs, SWCNT-PC and SWCNT-PCn. SWCNT-PC is grafted by a small MPC moiety, while for SWCNT-PCn, the PCn moiety formed a chain structure attached on the SWCNTs. We further grafted fluorescein isothiocyanate (FITC) onto two modified SWCNTs to make these two samples fluorescent, labled SWCNT-PC-FITC and SWCNT-PCn-FITC.We investigated the cytotoxicity of the modified SWCNTs for three kinds of cell lines, Human Umbilical Vein Endothelial Cells (HUVEC), murine macrophage (RAW264.7) and murine fibroblast (L929). These cell lines are typical models widely used in studies of cytotoxicity, endocytosis, metabolism, proliferation and RNA expressions. It was found that the modified SWCNTs possess a relatively lower cytotoxicity for the three cell lines compared with prestine SWCNTs, moreover, the three cell lines exhibited different tolerance against the modified SWCNTs.In this work, fluorescence microscope was applied to observe the three cell lines incubated with SWCNT-PCn-FITC both at37℃and4℃. This experiment was performed in order to confirm the results of CCK-8assay and further invesitigate the mechanism of cytotoxicity and uptake of modified SWCNTs. Moreover, we tried to find the relationship between the SWCNTs cytotoxicity and its uptake mechanism, we found that the cytotoxicity of modified SWCNTs was underestimated in CCK-8assay, and the cytotoxicity was closely related with the uptake mechanism of SWCNTs.

Related Dissertations

  1. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  2. Synthesis and properties of new functionalized graphene oxide drug carriers,TQ460.4
  3. Functional Study of Active Sites in Scorpion Insect Toxin BmK IT from Buthus Martensii Karsch,Q51
  4. The Syntheses and Applications of Nanao Cadmium Sulfide Based on the Green Chemical Concept,O614.242
  5. Oxya endogenous inhibitory protein isolates SDLH feasibility study as a preservative,TS202.3
  6. Research North Qinglongyi cytotoxic effect relationship of the active site of the spectrum,R284
  7. Preparation and Characterization of Fluorescent Carbon Nanoparticles and Their Application in the Cell Labeling,TB383.1
  8. Preparation and Evaluation of Dry Powder Inhalation of Extract of Trollius Chinesis Bungle,R283
  9. Structure Modification of Antitumor Activity Composition in Solanum Nigrum and Its in Vitro Antitumor Cytotoxicity Evalution,R285
  10. Synthesis and Anti-tumor Activities of N’-benzylidene-2-(4-oxothieno [2,3-d] Pyrimidin-3 (4H)-yl) Acetohydrazone Derivatives,R96
  11. Glycosidal Structure Modification and Evaluation in Vitro Cytotoxicity of the Glycoside of Solamargine,R285
  12. Toxicity of Silver-nanoparticles (NPs) and Silver-NP Based Hydrogel,TB383.1
  13. Studies on the Chemical Constituents and Bioactivities of Sponge Haliclona Cymaeformis and Topsentia.sp.from the South China Sea,R284
  14. Quantitative and Functional Changes of Peripheral Natural Killer Cells and Regulatory T Cells in Women with Reproductive Failure after Artificial Insemination with Donor Sperm,R711.6
  15. Live Cell Imaging and Cytotoxicity of Tetraphenylethene with Aggregation-induced Emission Characteristic,R318.0
  16. Studies on the Chemicalconstituents of Euonymus Acanthocarpus,R284
  17. Studies on the Constituents from Celastrus Rugosus,R284
  18. The Establishment and Application of Methods for Toxicity Detection of Human Fecal Water,R446.1
  19. 一. Lipid Rafts and the Forward Signal of Two Types of TNF-α 二. Construction of Topic Correlative TNFR1 Gene, Mutants and Mcs of pTriEx4.0 Vector and Trimerization Mutant,Q78
  20. Studies on Genotoxicity of Two Novel Contraceptive Materials,R114
  21. Study on the Chemical Constituents and Bioactivities of Acalypha Brachystachya and Daphne Genkwa,R284

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering
© 2012 www.DissertationTopic.Net  Mobile