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Research of the Effect to the Occurrence and Development of T2DM and Its Associated Vascular Complications of Mannose-binding Lectin and Inflammatory Factor and the Association between Them

Author: MaDanDan
Tutor: LiuGeLing
School: Hebei Medical University
Course: Internal Medicine
Keywords: Mannose-binding lectin(MBL) PCR-RFLP Gene poly-morphism Type2Diabetes Mellitus(T2DM) Vascular complications High-sensitivity C-reactive protein (hs-CRP) Homocysteine(Hcy)
CLC: R587.1
Type: Master's thesis
Year: 2013
Downloads: 16
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Abstract


Objective:In this research,we detect the gene polymorphism ofmannose-binding lectin(MBL) and the concentration of MBL in serumwith the techniques of polymerase chain reaction-restriction fragmentlength polymorphism (PCR-RFLP) and enzyme-linked immunesorbentassay(ELISA). We analyze the changes in the gene polymorphism ofmannose-binding lectin(MBL),the concentration of MBL in serum,andthe the level of high-sensitivity C-reactive protein (hs-CRP),homocysteine(Hcy),the sulfatide and biochemical indicators,to explorethe relationship between the above factors and T2DM and itsmacrovascular and microvascular complications. To explore the possiblepathogenesis of diabetes and its associated vascular comp lications fromthe point of view of the innate immune and inflammatory response andthe association between them,to offer a new way of prevention andtreatment and the theoretical basis of diabetes.Methods:1.Specimen collection:the objects of this research is174patient withT2DM from Tangshan workers hospital. To collect their fasting venousblood,with patients with T2DM(without macrovascular or microvascularcomplications)58cases(group D1),30male,28female,aged from36to72years;56cases of T2DM with diabetic nephropathy(group D2),29male.27female,aged from33to75years;60cases of T2DM with macro-vascular(group D3),30male,30female,aged from30to80years.And wecollect fasting venous blood of100cases as healthy controls in the sameperiod from medical examination center of Tangshan workers hospital. 2.To detect the gene polymorphism of mannose-binding lectin(MBL)in group H,D1,D2and D3,with the techniques of PCR-RFLP. Tocompare the statistics distinction between different groups. To comparethe statistics distinction between different groups.3.ELISA was used to detect the concentration of MBL in serum ingroup H,D1,D2and D3. To analyze the depend-ability between thechanges of the density and T2DM and its associated vascularcomplications.4.To collect the level of hs-CRP、Hcy、the sulfatide and otherbiochemical indicators,to compare the significant difference between thecomparison group.To analyze the relation of the above factors and T2DMand its associated vascular complications.Results:1.MBL gene exon I point mutation at54codon was detected byPCR-RFLP analysis, the results showed: MBL gene exon I pointmutation at54codon were no significant differences (P>0.05) among thefour groups of H, D1, D2and D3.2.The level of serum MBL was detected by ELISA, the resultsshowed:the level of serum MBL of group H was2.93±0.82mg/L, thelevel of serum MBL of group D1was2.90±0.83mg/L,the level of serumMBL of group D2was3.95±0.65mg/L,the level of serum MBL of groupD3was3.97±0.35mg/L, MBL serum levels showed no significantdifference between the D1group and H group (P>0.05), MBL serumlevels in D2, D3group was significantly higher than the D1group and Hgroup, the difference was statistically significant (P <0.05).3.The levels of serum hs-CRP and Hcy:the level of serum hs-CRPand Hcy of group H were1.58±0.97mg/L and10.18±2.44μmmol/L, thelevel of serum hs-CRP and Hcy of group D1were2.94±1.24mg/L and15.67±3.93μmmol/L, the level of serum hs-CRP and Hcy of group D2were4.67±1.76mg/L and22.68±6.96μmmol/L, the level of serumhs-CRP and Hcy of group D3were4.55±1.38mg/L and 24.59±7.33μmmol/L,the levels of serum hs-CRP and Hcy were inascending order of group H,D1and D2,D3, the differences werestatistically significant (P <0.05).4.The level of TC,TG and LDL-C: the level of serum TC,TG andLDL-C of group H were4.68±0.79mmol/L,1.57±1.17mmol/L and1.21±0.57mmol/L, the level of serum TC,TG and LDL-C of group D1were5.21±0.97mmol/L,2.16±0.98mmol/L and3.17±0.93mmol/L, thelevel of serum TC,TG and LDL-C of group D2were5.86±0.97mmol/L,2.83±1.72mmol/L and4.39±1.17mmol/L, the level of serumTC,TG and LDL-C of group D3were5.93±1.16mmol/L,2.95±1.41mmol/Land3.68±1.04mmol/L, the levels of serum TC,TG and LDL-Cwere in ascending order of group H,D1and D2,D3, the differences werestatis-tically significant (P <0.05).5. The level of HDL-C:the level of serum HDL-C of group H was1.33±0.30mmol/L, the level of serum HDL-C of groupD1was1.11±0.27mmol/L, the level of serum HDL-C of groupD2was0.96±0.18mmol/L,the level of serum HDL-C of groupD3was0.83±0.16mmol/L, the levelof serum HDL-C was in descending order of group H,D1and D2,D3, thedifferences were statis-tically significant (P <0.05).Conclusions:1. MBL gene exon I point mutation at54codon had no different ingroup H,D1,D2and D3,suggested that:the gene polymorphism of MBLgene exon I54codon may be unrelated with T2DM and its associatedvascular complications.2.The serum MBL were higher in patients with T2DM′s associatedvascular complications were higher than them with T2DM only(withoutmacrovascular or microvascular complications) and the control group.Suggested that:the higher level of serum MBL may be associated with theincidence of macrovascular complications and diabetic nephropathy ofT2DM, Which provided a theoretical basis for clinical intervention ofserum MBL levels to delay the occurrence and development of diabetes-related complications.3.The level of serum hs-CRP、Hcy、TC、TG and LDL-C increasedin patients with T2DM, especially in patients with diabetes-relatedmacrovascular complications and diabetic nephro-pathy,suggesting that:the above factors may be risk factors of T2DM and its macrovascular andmicrovascular complications; The level of serum HDL-C reduced inpatients with T2DM, especially in patients with diabetes-relatedmacrovascular complications and diabetic nephro-pathy,suggesting that:HDL-C may be protective factors of T2DM and its macrovascular andmicrovascular complications,providing a theoretical basis ofmultifactorial clinical intervention to prevent and delay the incidence ofdiabetes and its associated vascular complications effect ively.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
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