Dissertation > Excellent graduate degree dissertation topics show

Protective Effects of Berberine on High-fat Diet Induced Chronic Kidney Injury and Relative Mechanisms’ Research

Author: ZhaoZuo
Tutor: CaoChangChun
School: Nanjing Medical University
Course: Internal Medicine
Keywords: Berberine High-fat diet Macrophage Chronic kidney injury NF-κB
CLC: R692
Type: Master's thesis
Year: 2013
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


Background Metabolic syndrome (MS) is a clinical syndrome characterized with acomplex metabolic disorder, including hypertension, hyperglycemia, hyperlipidemia,insulin resistance etc. Chronic kidney injury is closely associated with metabolicsyndrome. Hyperlipidemia, one important component in MS, not only aggravatesvarious kinds of kidney diseases, but also directly induces kidney injury. IKK/NF-κBsignaling pathway is a major inflammatory signal transduction pathways. Thestimulation of the lipid activates IKK/NF-κB pathway, then actives NF-κB, andthrough the scecretion of inflammatory cytokines causes the damage to the cells.Berberine (BBR) depended on its unique pharmacological effects has become aresearch hotspot in recent years. But how to reasonably apply berberine in thetreatment of chronic kidney disease (CKD) is worth exploring.Methods Feeding C57BL/6mice with a high-fat diet was an available way toinvestigate metabolic syndrome. In order to explore the protective effect of berberineand the related mechanisms’, we established the animal models of mice chronickidney injury and observed the changes of serum lipids, renal function, renal tissuemorphology and the expression of inflammatory and fibrosis factors in each groups.We fed C57BL/6mice with high-fat diet to induce chronic kidney injury. Then themice of model group were intragasticed150mg/kg·d berberine dissolved in1%carboxymethylcellulose sodium (CMCC-Na) for six weeks after animal modelsestablished. At the end of the experiment, blood and kidney tissue were collected.Blood was for dectecting urine volume, urinary albumin, total cholesterol,triglyceride, cystamin C, serum creatinine and urea nitrogen. Pathological changeswere observed by light microscope by HE staining. Expression of phosphorylatedIKKβ, phosphorylated IKKε, phosphorylated p65, phosphorylated p50, inflammatoryfactors (IL-1β, iNOS) were detected by Western Blot. Expression of F4/80and iNOSwere detected by immnofluorescence staining staining and immunohistochemistry respectively. Then the ratio of mcrophage-positive in the section of kidney wascalculated.Results The research shows that six weeks’ treatment of berberine (150mg/kg·d) isable to effectively improve the renal abnormalities, including elevating renal function,ameliorating renal morphology, reducing the infiltration of mononuclear macrophages,inhibiting the expression of pIKKβ, pIKKε, phosphorylated p65, phosphorylated p50and inflammatory factors. These protecive effects may regulated by NF-κB signalingpathway.Conclusions Berberine may through the NF-κB signaling pathway to reduce theinfiltration of mononuclear macrophages, especially the M1mononuclearmacrophages, suppress the inflammatory reaction and then further ameliorate themice chronic kidney injury induced by high-fat diet. The research provides a novelaprroach and theoretical basis for the treatment for kidney diseases associated withmetabolic syndrome.

Related Dissertations

  1. Study on the Combined Probiotics and Its Effects on Prodution Performance of Pigs,S828.5
  2. Synthesis and properties of new functionalized graphene oxide drug carriers,TQ460.4
  3. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  4. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  5. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363
  6. Effect of High Glucose on Chemokine Ligand 16 (CXCL16) Expression and Secretion in THP-1 Macrophages and Intervention of Aspirin in the Activity,R587.2
  7. The Molecular Mechanism Intervention of Selenium on the Learning and Memory Injury by Drinking Water Fluorosis,R599
  8. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  9. Effects of Partial Hepatic Ischemia/Reperfusion Injury on Postoperative Cognitive Function in Mice,R614
  10. Experimental Study of Influence of Atrovastatin on Expression of NF-κB in Retionpathy of Diabetic Rats,R587.1
  11. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  12. The Sequence Analysis of RNA Components from Lactobacillus Metabolites and Parts of the Biological Function,R378
  13. Study on Advanced Oxidation Technology of Ozonation for Berberine Pharmaceutical Wastewater Treatment,X787
  14. Study on Therapy and Mechanisms of the Role of Tang Kang in Diabetic Rat and Endothelial Cells,R285.5
  15. Effects of Hypothermia Resuscitation on PPAR-γ and Related Gene Expression in Rats Lung during Hemorrhagic Shock,R605.971
  16. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  17. The Research of Nf-kB、TNF-a and PLGF in the Pathogenesis and Severity of Preeclampsia and Theirs Relevance,R714.244
  18. The Effect of Berberine Hydrochloride on the Levels of Serum CRP and Complement C3, C4 in Patients with Acute Cerebral Infarction,R743.3
  19. Influence on NF-κB P65 and ASPP2 of Murine Lewis Lung Cancer of NF-кB Inhibitor PDTC and Cisplatin,R734.2
  20. Restructuring Agkistrodon disintegrin Adinbitor human non-small cell lung cancer cell line A549,R285.5
  21. Berberine on serum levels of superoxide dismutase and malondialdehyde levels in,R743.33

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease
© 2012 www.DissertationTopic.Net  Mobile