Dissertation > Excellent graduate degree dissertation topics show

Analysis of Common Pathogen and Inflammatory Markers of Children with Lower Respiratory Tract Infection in Winter and Spring

Author: MaJing
Tutor: ZhangHuiFeng
School: Hebei Medical University
Course: Pediatrics
Keywords: Children with lower respiratory tract infections etiology high-sensitivity C-reactive protein leukocytes proportion of neutrophils
CLC: R725.6
Type: Master's thesis
Year: 2013
Downloads: 28
Quote: 0
Read: Download Dissertation

Abstract


Objective: To understand the status of the etiologic spectrum of hospitalizedchildren with acute lower respiratory tract infection (ALRI) in winter andspring,provide a basis for virological diagnosis of children with ALRI inShijiazhuang and surrounding areas.Methods:84cases of children with ALRI were collected as the researchobject, who were hospitalized in the Pediatric Department, the second hospitalof Hebei Medical University from January2011to May2011, including56boys and28girls. All of the subjects were divided into three groups: group ofinfants (less than1year old) in41cases, the child care group (1to3years old)in26cases, the group of children (more than3years of age) in17cases. Allthe cases were collected the venous blood2ml for the test of nine commonrespiratory pathogen antibodies using indirect immunofluorescence the nextday of hospitalization, including the five respiratory viruses: respiratorysyncytial virus(RSV),influenza A virus (IFVA),influenza B virus (IFVB)andparainfluenza virus (PIV), adenovirus (ADV);four atypical pathogens:Mycoplasma pneumonia (MP),Chlamydia pneumonia (Cpn), Legionellapneumophila type1(LP1), Richettsia query (QFR). And we collected theperipheral blood for the test of the blood routine and high-sensitivityC-reactive protein (hsCRP). Viewed the distribution of nine kinds ofpathogens as well as its popular features related to age, sex. Divided the casesinto RSV-IgM positive group and MP-IgM positive group. Chose the caseswhich were negative but with high hsCRP as the control group. Compare thedifferences of hsCRP and white blood cells, neutrophils ratio among the threegroups.All statistical analyses were performed with statistical softwareSPSS13.0, applyed t test, rank sum test, chi-square test and so on. Dates which were normally distributed were showed as mean±standard deviation(x±s),others were showed as median (interquartile spacing). P<0.05was consideredstatistically significant.(If hsCRP<0.05mg/L, we calculated it as0.04mg/L.)Results:1Children of subjects of the84cases, the pathogen antibody waspositive in47cases, accounting for55.95%(47/84); respiratory virus antibodywas positive in38cases, accounting for45.24%(38/84);single virus antibodywas positive in31cases, accounting for36.9%(31/84), and RSV antibodypositive accounted for21.43%(18/84), IFVB accounted for13.1%(11/84),PIV accounted for2.38%(2/84). Atypical pathogens antibody waspositive in16cases, accounting for19.05%(16/84),all the cases were MPantibody positive, of which the single MP antibody positive was in9cases,accounted for10.71%(9/84). The mixed infection (viral and atypicalpathogens in mixed infections) was in8cases, accounting for9.52%(8/84),all the cases were MP merge other viral infections.2The relationship betweengender and the positive rate of the common viruses and atypical pathogen:themale children with virus-positive rate was44.64%(25/56),and the femalechildren with virus-positive rate was46.43%(13/28), there was no statisticallydifference(P<0.05); the male children with atypical pathogen-positive rate was19.64%(18/56),and the female children with atypical pathogen-positive ratewas17.86%(5/28), there was no statistically difference(P<0.05).3Therelationship between age and the distribution of common viruses, atypicalpathogens and mixed infection: The study of84patients, age was1.0(1.98)years, of which a minimum of43days, the biggest11-year-old. The atypicalpathogens positive rate in infant and younger children group(less than3yearsold) was7.46%(5/67), and that in children’s group was64.71%(11/17), Thechildren group atypical pathogens detection rate was significantly higher thaninfants, the difference was statistically significant(X2was1.187,P<0.05).4The RSV-IgM antibody positive rate was highest in the group of infants,51.2%, and the child care group with children group were11.54%(3/26) and11.76%(2/17), the difference was statistically significant(X2was7.207,P<0.05);the MP-IgM antibody positive rate which was accounted for 64.7%(11/17),was higher than child group, accounted for7.46%(5/67), thedifference was statistically significant (X21.187, P <0.05). The children’sgroup of influenza B virus-positive rate was slightly higher than the other twogroups.5The hsCRP, white blood cell value, and the proportion ofneutrophils of MP-IgM positive group were lower than bacterial infectiongroup. The hsCRP, WBC value, and the proportion of neutrophils ofRSV-IgM positive group were significantly lower than the bacterial infectiongroup; these differences were statistically significant(P<0.05).Conclusion:1Pathogens of the lower respiratory tract infection in this regionin winter and spring were mainly viruses, and single common respiratory virusinfection was mainly to respiratory syncytial virus infection, and theninfluenza B virus and parainfluenza virus. Respiratory syncytial virusinfection is more common in infancy. The infection of Atypical pathogens wasmainly Mycoplasma pneumoniae, of which the positive rate was the highest inchildren group. Mixed infection (viral and atypical pathogenicmicroorganisms mixed infection) was mainly Mycoplasma pneumoniaeassociated with other viral infections.2Respiratory syncytial virus (RSV) is the mainly pathogen in infant groupwith lower respiratory tract infections in the winter and spring in this region.Mycoplasma pneumoniae is the mainly pathogen in children group with lowerrespiratory tract infections in the winter and spring in this region. Mycoplasmapneumoniae infection can lead to blood hsCRP, white blood cells andneutrophil ratio elevated. The hsCRP, WBC value, and the proportion ofneutrophils of RSV-IgM positive group were significantly lower than thebacterial infection group.3In this study, we used indirect immunofluorescence to detect the commonnine pathogens specificial IgM antibody of respiratory. It has the advantagesof high sensitivity, good reproducibility, easy to operate, fast, and can be as early diagnosis of infection.

Related Dissertations

  1. Change and Value of Serum Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 in Acute Coronary Syndrome,R541.4
  2. Effects of Telmisartan on hs-CRP and HMW-adiponectinin Patients with Essential Hypertension,R544.1
  3. Application and Significance of CISS Classification and TOAST Subtype on Ischemic Stroke (Attach 210 Cases Analysis),R743.3
  4. Ankle-brachial index combined ultra- sensitivity C-reactive protein determination of the value of coronary artery disease,R541.4
  5. Individuals with type 2 diabetes, serum complement factor H, hs-CRP levels and insulin resistance study,R587.1
  6. Clinical Analyses of 148 Cases of Cerebral Infarction in Young Adults,R743.3
  7. Relationship between Acute Hyperglycemia and Prognosis in Patients with Acute Myocardial Infarction,R542.22
  8. Congenital Hypothyroid Function Thyroid Screening Analysis,R725.8
  9. The Expression of B7-H4 in Human Colon Carcinoma and Its Clinical Significance,R735.3
  10. Research Progress of Kawasaki Disease,R725.4
  11. S100A12 in Coronary Atherosclerotic Heart Disease Expression and Significance,R541.4
  12. Study of Serum Endothelin and High Sensitivity c-reactive Protein in Type 2 Diabetic Patients with Hypertension,R587.1
  13. Clinical Analyses of 235 Cases of Neonatal Vomiting,R722.1
  14. Prostate Secretion Laboratory Inspection in Chronic Prostatitis Etiology Research,R697.33
  15. To Researched the Impact of Rosuvastatin on Adiponectin, Visfatin and High Sensitivity C-reactive Protein in Patients with Metabolic Syndrome,R589
  16. Acute Suppurative Thyroiditis Misdiagnosed as Subacute Thyroiditis: A Case Report and Literature Review,R581.4
  17. Inflammatory and coagulation factors and CRP1059G / C gene polymorphism and pulmonary thromboembolism associated,R563.5
  18. Etiology and Metabolic Disorders Analysis for Hospita Lized Hypertensive Patients in Report from the Depart Ment of Hypertension,R544.1
  19. Analysis of Etiology of In-patients with Hypertension from Grade AAA Hospital in Xinjiang,R544.1
  20. A Retrospective Investigation of Clinical Cases of Fever of Unknown Origin in Beijing University Shenzhen Hospital in Recent Ten Years,R441
  21. The application of the theory of collateral disease in systemic lupus erythematosus,R259

CLC: > Medicine, health > Pediatrics > Children within the science > Department of pediatric respiratory and chest diseases
© 2012 www.DissertationTopic.Net  Mobile