Dissertation > Excellent graduate degree dissertation topics show

Expression of MACC1in Rectal Adenocarcinoma and Its Correlation with Epithelial-mesenchymal Transition

Author: ZhangXiHua
Tutor: GuanGuoXian
School: Fujian Medical
Course: Oncology
Keywords: MACC1 Rectal adenocarcinoma Epithelial-mesenchymal transition
CLC: R735.3
Type: Master's thesis
Year: 2012
Downloads: 57
Quote: 0
Read: Download Dissertation

Abstract


AIM: To investigate the expression of MACC1in rectal adenocarcinoma and therelationship between MACC1and epithelial mesenchymal transition of rectaladenocarcinoma.Methods: Reverse transcriptase polymerase chain reaction (RT-PCR) was used todetermine the expression of MACC1, E-cadherin and vimentin mRNA in30pairs ofrectal adenocarcinom and the adjacent normal tissues. Immunohistochemistry wereused to examine the expression of MACC1, E-cadherin and vimentin protein in90specimens of rectal adenocarcinoma and30specimens of adjacent and analyse therelationship of them.Results: The RT-PCR examination showed that the relative expression level in rectaladenocarcinoma tissues were significant higer than that in normal rectum tissues(0.535±0.310vs0.373±0.190, P<0.05); By Immunohistochemistry examination, thepositive rates of MACC1in rectal adenocarcinoma tissues were also significantlyhigher than that in the normal tissues(86.7%vs36.7%, P<0.01). By univariate analysis,the overexprossion of MACC1protein was correlated with the clinical stage anddistant metastasis(P<0.05), but not to patients age, sex, tumour size,Differentiation degree, lymph node metastasis(all P>0.05). Cox regressionmultivariate analysis showed that MACC1positive expression is an independentfactor for the prognosis of rectal adenocarcinoma. The Pearson’s correlation analysisdemonstrated that MACC1expression was negatively correlated with E-cadherinexpression(r=-0.326;P=0.002), while it was positive correlation with Vimentinexpression(r=0.284;P=0.007).Conclusions: MACC1is up-regulated expression in rectal adenocarcinoma,and isclosely related to tumor metastasis, it is an independent factor for the prognosis ofrectal adenocarcinoma. MACC1may be involved in epithelial mesenchymal transition of rectal adenocarcinoma.

Related Dissertations

  1. Effect of YB-1 Downregulation Via RNAi on MACC1 Gene Expression in Human Lung Adenocarcinoma Cell Line A549,R734.2
  2. Effect of Astragalus and Salvia’s Effective Components and Their Compatibility on JAK/STAT Pathway of Renal Fibrosis,R285.5
  3. Expression and Implication of Cripto-1、 E-cadherin、 β-catenin and vimentin in Ovarian Serous Adenocarcinoma,R737.31
  4. Relationship between Epithelial-mesenchymal Transition Induced by Hypoxia and Invasive Ability in Hepatocellular Carcinoma Cells,R735.7
  5. To Seek the Different Proteins in Ovarian Cancer Cells by Two-dimensional Electrophoresis,R737.31
  6. The Effect of MACC1 on Invasion and Metastasis in Prostate Cancer Cell Lines (1Ee8/2B4),R737.25
  7. Screening and Identification of Metastasis-associated MicroRNAs in Human Hepatocellular Carcinoma,R735.7
  8. Regulating of Epithelial-mesenchymal Transition to the Volume of Side Population Cells in Human Ovarian Cancer,R737.31
  9. To Study the Correlation of Caspase 8,9 and EMT Regulating Proteins in the Early Stage of Tumor Microcirculation of the Tumor Cells Around Lineraly Patterned Programmed Cell Necrosis and Vasculogenic Mimicry,R730.2
  10. The Effect of TGF-β1-mediated Rho/ROCK Signaling Pathway on Tight Junctions of HK-2 Cells,R692
  11. The Mobility of Normal Kidney Epithelial Cell in the Course of Epithelial-mesenchymal Transition (EMT),R692
  12. Clinical Significance and Expression of GRP78 and GRP94 in Human Rectal Adenocarcinoma,R735.37
  13. The Expression and Meaning of MicroRNA-143 and C-myc in the Rectal Adenocarcinoma,R735.3
  14. Expression of Transcriptional Facter Snail in NSCLC and the Relation with EMT,R734.2
  15. Integrin-linked Kinase and Epithelial-Mesenchymal Transition in the Pathegenesis of Pulmonary Fibrosis,R563.9
  16. Study on Epithelial-Mesenchymal Transition and Osteopontin in Mice Bleomycin-induced Pulmonary Fibrosis,R563
  17. The Effects and Mechanism of Napsin A Gene Therapy for Pulmonary Fibrosis,R563.9
  18. Anti-renal Fibrosis Research of Astragali Radix and Salviae Miltiorrhizae Radix Et Rhizoma Compatibility and Their Effective Components,R277.5
  19. The Regulation Mechanism of ACE2in the Hepatocytes Epithelial Mesenchymal Transition Induced by Angiotensin Ⅱ,R575.2
  20. Salvianolic Acid B Prevents TGF-β1-induced Hk-2Cell Epithelial-mesenchymal Transition and Changes Related microRNAs’ Expression,R692.6
  21. Effect of Erythropoietin on Albumin Induced Transition in Human Kidney Proximal Tubular Epithelial Cells and the Relationship between TGFβ1/ILK Signaling Pathway,R692

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms
© 2012 www.DissertationTopic.Net  Mobile