Dissertation > Excellent graduate degree dissertation topics show

Assessment of Resistance Risk and Resistance Machanism in Spodoptera Exigua(H(u|¨)bner) to Chlorantraniliprole

Author: LiJia
Tutor: SuJianYa
School: Nanjing Agricultural College
Course: Pesticides
Keywords: Spodoptera exigua chlorantraniliprole resistance risk synergist detoxification enzymes
CLC: S482.3
Type: Master's thesis
Year: 2013
Downloads: 2
Quote: 0
Read: Download Dissertation

Abstract


In this paper, chlorantraniliprole resistance was selected in S. exigua, the resistance risk was estimated. The resistance to other insecticides of this strain, the change of detoxification enzymes activities after selection and the influence of chlorantraniliprole sublethal dose to detoxification enzymes activities in S. exigua were studied.1. Assessment of resistance risk in S. exigua to chlorantraniliproleBased on22generations of chlorantraniliprole selection in S. exigua, writer was in charge of selection from23to48generation, and the LC50raised from1.8821mg/L to6.3807mg/L with3.4-fold increase.Compared with the susceptible strain, the resistance ratio had reached465.7-fold.The realized heritability (h2) of resistance was estimated as0.0597by using threshold trait analysis, when the slope is2.30, h2is0.0597,70%-90%of the population is killed at each generation,10-fold increase in LC50need21.9-31.1generations, indicating that the risk of resistance development exists in S. exigua to chlorantraniliprole after continuous application.2. The resistance to conventional insecticides of selected strainUsing leaf dipping bioassay to determine12conventional insecticides resistance of selected strain, the results showed that the resistance to chlorpyrifos (RR>12526.8), cyhalothrin (RR>864), chlorfluazuron (RR=679.2), metaflumizone (RR=535.9), indoxacarb (R-R=151.7), pyridalyl (RR=47.7) were very high, the resistance to spinosad and methomyl belong to moderate resitance with resistance ratio of23.7and11, and the resistance to endosulfan, flubendiamide, emamectin enzoate were low with RR of9.9,7.7,1.9respectively. As the original population of selected strain was collected on Brassica rapa chinensis in Shanghai in2008, low and moderate resistance maybe caused by different genetic and environmental background with susceptible strain, high level resistance to7conventional insecticides was related to original population’s resistance developed during the long-term application of some commonly used insecticides before2008, which is also maybe the reason of some novel pesticides’s resistance, such as metaflumizone and Pyridalyl. All the resistance above didn’t reverse during the selection, so they don’t suggest to be used mixing with chlorantraniliprole.In order to investigate the resistance biochemical mechanism, PBO, DEF, DEM and methimazole were used with five insecticides. DEF significately synergized chlorantraniliprole with synergism ratios (SR) of2.3, means the esterase involved in the detoxification mechanism to chlorantraniliprole in S.exigua. DEF, DEM, methimazole showed2.6-,2.7-,2.2-fold SR on metaflumizone respectively, indicating that esterase, glutathione-S-transferase enzymes and FMO may involve in the detoxification of S. exigua to metaflumizone.Pretreating with PBO, the toxicity of cyhalothrin was significately synergized with SR of3.6, means P450plays an important role in the high resistance to pyrethroid in S. exigua. Methimazole obviously synergized emamectin benzoate (SR=2.7), maybe because compared with the concentration of synergist, emamectin benzoate’s LC50was low, which can provide guidance for synergist concentration selection in the future.3. The influence of chlorantraniliprole’s sublethal dose to detoxification enzymse in the body of the beet armywormThe weight of larvae which exposed to chlorantraniliprole’s LC15maitained around2mg, and the development delayed obviously, while larvae weight of control group increased signicantely. All the detoxification enzymes activities in the control group almost didn’t change at first, and went up later. P450had a significant rise after16h, FMO raised after24h, esterase significantly increased after72h, GST increased after36h. But all the detoxification enzymes activities in the treated group were almost unchanged during the96h, indicating that they all can’t be induced by chlorantraniliprole. But esterase involved in detoxification to chlorantraniliprole in the selected strain, maybe because the selective pressure killed larvaes whose detoxification enzymes activities were low.

Related Dissertations

  1. The Amide study of chlorine insects,TQ453.2
  2. Assessment of Resistance Risk in Spodoptera Exigua (Hubner) and Spodoptera Litura (Lepidoptera: Noctuidae) to Chlorantraniliprole,S433
  3. Incidence and Mechanisms of Insecticides Resistance to Pyrethroids and Endosulfan in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32
  4. Monitoring of Insecticide Resistance, Resistance Risk Assessment and Biochemical Mechanism for Buprofezin in Nilaparvata Lugens(St(?)l),S435.112.3
  5. Foraging Behaviors of Meteorus Pulchricornis for Spodoptera Exigua Larvae,S476.3
  6. Insceticide Screening and Resistance Risk Assessment for Thiamethoxam in Western Flower Thrips, Frankliniella Occidentalis,S433
  7. Susceptibilities to Four Neonicotinoids and Related Tolerant Mechanisms in Colorado Potato Beetles (Leptinotarsa Decemlineata),S435.32
  8. Bioactivities of Shenqinmycin Against Xanthomonas Oryzae PV. Oryzae and Sclerotinia Sclerotiorum and the Resistance Risk,S435.111
  9. Molecular Chracterization and Tissue Expression of Cytochrome P450 and Esterase Gene Fragemets in the Antennae of Spodoptera Exigua (H(?)bner) and Spodoptera Litura (Fabricius),S433.4
  10. The Influence of Coumaphos on Enzyme Activities of Antheraea Pernyi and Blepharipa Tibialis Chao,S885.1
  11. Pb to Silkworm anti-oxidation and related gene expression as well as reproductive and developmental impact of activity,S884.9
  12. Study on Resistance Monitoring and Its Resistance to Beta-cypermethrin in Spiraling Whitefly,S433.3
  13. Coal-fired power plant flue gas desulphurization synergist application technology research,X701.3
  14. CTF synergist impact on the performance of cement-based materials,TU528
  15. Analysis of Drug Resistance of Pathogens of Nosocomial Infections in Critically Ill Patients and Risk Factors,R446.5
  16. Shihezi bollworm resistance monitoring and CYP6AE14 and AK gene,S435.622.3
  17. Effects of Sublethal Concentration of Three Acaricides on Laboratory Population and Detoxifying Enzymes of Tetranychus Turkestani Ugarov Et Nikolski,S433
  18. Research on Capabilities of High Temperature Resistance and Cold Tolerance of Immature Instars in Changsha and Some Laws of the Body Color Changes of Beet Armyworm,S433
  19. Susceptibility of Sesamia Infernes (Walker) of Field Populations to Various Insecticides and Resistance Mechanisms,S435.112.1
  20. Studies on Phenylpyrazole Insecticide Resistance Risk Assessment and Cross-Resistance in N. Lugens,S435.112.3
  21. Colning and Expression of Heat Shock Protein Genes (hsp70 and Hsp90) of the Beet Armyworm, Spodoptera Exigua (lepidoptera: Noctuidae),S433

CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Pesticides
© 2012 www.DissertationTopic.Net  Mobile