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The AFLP and SSR Markers Analysis Carp (Cyprinus Carpio L) Growth of the Genetic Structure and Genetic Differences at Different Stages

Author: DanYunJing
Tutor: SunXiaoWen
School:
Course: Biochemistry and Molecular Biology
Keywords: Carp Markers SSR. Different growth stage Genetic differences Genetic structure
CLC: S965.116
Type: Master's thesis
Year: 2014
Downloads: 3
Quote: 0
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Abstract


On carp genetic diversity analysis, found that many tag unbalanced phenomenon;On carp genetic mapping and QTL analysis based on the single family, found a lot ofdeviating from the balance of the site, suggesting that a certain genotype gravelydeviates from the Mendelian balance ratio, for the reason of this phenomenon is notclear. This research use efficient markers and SSR molecular marker technology, scan asingle family carp different growth stages (the film, the film after swim before, flat,autumn when adult salmon) genome DNA, carp out part of the genotype differencesbefore and after the film is analyzed; Deviating from the balance of the tag is used toanalyse the different development phase analysis, obtained the single family carpgenetic diversity of the data, compare the growth of four stages of genetic structure andgenetic differences, so as to reveal the unbalanced phenomenon began to appear in thewhich stage and change rule of genetic diversity. Molecular biology of carp basicresearch has important theoretical and practical significance, at the same time, also toassess carp germplasm resources, which provides the basis for establishing database ofits genetic diversity. The main results were as follows:This research use markers markers, peace tour before the two stages of themembrane of normal and poor condition (deformity group) were analyzed. By siftingthrough64pairs of primer combination, and found that there are15primers in fourgroups differences stripe. Main results and conclusions are as follows:1. The film before the average percentage of polymorphic loci was23.48%, slightlyhigher than the film after the average percentage of polymorphic loci was22.05%.Through the proportion of polymorphic loci, normal individuals with death or poorcondition difference. During the period of genetic distance are smaller at the same time,the genetic distance between membrane group before the minimum; Differentdevelopment stages of the genetic distance between populations is slightly greater thanthe same developmental stage population genetic distance.2. Through the screening,64pairs of primers, a total of35primers exist polymorphismloci;8primers exist differences banding including E1M3, E1M5, E1M6, E2M3, E2M4, E3M4, E4M4, E5M8, received13difference banding. In3299bits points, singletonsites3145,154average polymorphism loci polymorphism rate was4.67%.Polymorphic ratio is low.3. In the design of50to SCAR marker, positive for the three pair of primers cloning,the remaining47for false-positive cloning, there are3for false positivepolymorphisms of the cloning and44for singleton. False positive rate is higher.4. To get the positive clone sequences and some false positive sequence in the NCBIBLAST program sequence compares with other fish, the results were found functionsequence including: PEG-10protein and ATP dependent RNA helicase DDX4decoration gene, programmed cell death protein7analogues, interferon factor5,zebrafish cytokines signal suppression factor6analogues, homologous antigen Ma1.(2) the application of SSR molecular marker technique, this study from the early stageof the190map three representative is a rich source of imbalance in the hot spots fromthree linkage group31to polymorphic primers to carp in F2generation of four stages, atotal of384individual genotype analysis, get the phase change trend of geneticdiversity and build the four stages of linkage group, then compare the different growthstages of carp chain of the similarities and differences, mainly from the main results:1. Mirror carp genetic variation in the development of extreme from high to low aresort of A> B> C> D, this not only reflects the mirror carp has abundant geneticdiversity, and show that individual’s ability to adapt to environmental change strongeroffspring.2. Through the popgene3.2calculate H-W values to compare, according to the resultsin the table shows all the tags were greater than1meet the gerhard weinbergequilibrium, but presents a certain regularity and changes in the value, which can beroughly divided into three kinds of situations: gradually decline, steady developmenttrend, and gradually rising trend, gradually decline, these tag number of the total34.5%,46.7%and34.5%respectively.3. By clustering analysis can be seen that the mirror carp population four differentstages of development can be divided into two groups, one group is A and B phase firstget together again and C stage together for A big team, separately for A D stage. This was consistent with its development stage, namely the closer the development phase,the genetic distance is relatively small.4.190group mark most of young fish group in the new chain of chain, chain, part ofthe formation of tags can be found in groups of190marks. The change of the tagnumber of individual and group number, will affect the final accuracy of linkage map.Tag number, the more the greater the individual number, the result was a map ofresolution is higher, the more accurate.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquaculture technology > A variety of fish farming > Freshwater fish > Carp
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