Dissertation > Excellent graduate degree dissertation topics show

Study of Glucose-6-phosphate Isomerase from Sliver Carp and Blue Scad Skeletal Muscle

Author: ZhangChaoQun
Tutor: SuWenJin
School: Jimei University
Course: Of Food Science
Keywords: sliver carp blue scad glucose-6-phosphate isomerase myofibrillar proteins
CLC: TS254.1
Type: Master's thesis
Year: 2012
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


Modori phenomenon often occurs during the manufacturing process of surimi, affecting thequality of surimi products eventually. Researches have showed that modori effect was caused byendogenous proteinase, such as cathepsin B、L and myofibril-bound serine proteinase (MBSP).MBSP might be the major reason of the modori phenomenon. In order to produce high-qualitysurimi products, the efficient MBSPI are demanding in protecting myofibrillar proteins fromproteolysis by MBSP. In the present study, GPIs which could inhibit the activity of MBSP, wrepurified from the skeletal muscle of sliver carp (Hypophthalmichthys molitrix) and blue scad(Decapterus maruadsi), and we studyed the characters of the GPI. This will be a theory aboutimproving the quality of surimi products and using fish proteins effectively.Two GPIs were purified by ammonium sulfate fractionation, column chromatographies ofQ-Sepharose and SP-Sepharose from sliver carp and blue scad. The results showed that, the yieldof sliver carp GPI and blue scad GPI was6.6%and6.9%, and the purification fold was21.8and199.9respectively. Western blot analysis revealed positive reaction bands using rat againstwhite croaker MBSPI (GPI) polyclonal antibody.The optimal pH and optimal temperature of silver carp GPI was8.5and50℃; the optimalpH and optimal temperature of blue scad GPI was8.0and40℃, respectively. Eleven highersignal-noise ratio peptide fragments of the enzyme from silver carp were subjected toMALDI-TOF/TOF-MS/MS analysis, all the eleven peptide fragments revealed100%identity tophosphoglucose isomerase-2from zebrafish (Danio rerio), suggesting the purified enzyme fromsilver carp was GPI.GPI from silver carp revealed specific inhibitory activity toward myofibril-bound serineproteinase (MBSP) from crucian carp, while no inhibitory activity was identified toward pigtrypsin, pig chymotrypsin, snakehead trypsin, pacific white shrimp trypsin, mantis shrimp trypsin,silver carp trypsin and silver carp chymotrypsin. With the increasing of the concentration aboutblue scad GPI, the degradation of myofibrillar proteins was inhibited.When the addition of silver carp GPI reached0.031%of the minced fish meat, a9.2%increase of gel strength was dectected, while the addition of blue scad GPI reached0.024%,modori phenomenon occurred in the surimi products. These results strongly suggested that the endogenous MBSP inhibitor was effective and species-dependent.

Related Dissertations

  1. Seasonal Variation of Gut Cyanophyta Contents and Liver GST Expression of Four Freshwater Fishes in Tropical Xiangang Reservoir (Huizhou, China),S917.4
  2. Study Effect on Chemical Interactions of Myofibrillar Protein Gels,TS251.1
  3. Study on Transglutaminase-Induced Crosslinking on Gelation of Myofibrillar/Soy Protein Isolate Mixtures,TS201.2
  4. The Gene Encoding Putative Glucose-6-phosphate Isomerase (PGI) is Required for Full Virulence of Xamthomonas Campestris pv. campestris,S432.4
  5. Zooplankton Community Structure and Its Relation to Environment Factors in Qiandao Lake,Q958.8
  6. Effect of Pre-Emulsification on Emulsifying and Gelling Properties of Low-Saturated Fat-Protein System,TS251.1
  7. Mechanism of Crude Extract from Alaska Pollock and Beef Muscle in Tenderizing,TS251.1
  8. Effect of Protein Oxidation on Heat-Induced Gel and Physicochemical Properties of Chicken Breast Myofibrillar Proteins,TS251.1
  9. Physicochemical and Rheological Changes of Myofibrillar Proteins from Tuna (Thunnus Obesus) during Frozen Storage,TS254.1
  10. The Genetic Effect Evalution of Released Parent Hypophthalmichthys Molitrix,S931.5
  11. The Clinical Significance of Combined Detection Glucose-6-Phosphate Isomerase and Anti-Cyclic Citrullinated Peptide Antibody in Rheumatoid Arthritis,R593.22
  12. The Diagnosis Significant Gluocose-6-phosphate Isomerase in Patient with Rheumatoid Arthritis,R593.22
  13. Genetic Diversity of the Population of Phytophthora Infestans on Potato Crop in Yunnan Province, China,S435.32
  14. Serum Glucose -6- Phosphate Isomerase (GPI) Analysis in Patients with Rheumatoid Arthritis,R593.22
  15. A Study of Relationship between Glucose 6-phosphate Isomerase and Rheumatoid Arthritis,R593.22
  16. Patients with Rheumatoid Arthritis(RA) Serum and Synovial Fluid Levels of Glucose-6-phosphate Isomerase(G6PI) Antigen,R593.22
  17. Diagnostic Value of Rheumatoid Factor, Glucose-6-phosphate Isomerase and Anti-cyclic Citrullinated Peptide Antibodies for the Patients with Rheumatoid Arthritis,R593.22
  18. Studies on Chitosan and Its Derivatives Adsorption for Paralytic Shellfish Poisoning in Ostrea Rivularis Could,TS254.4
  19. The Study of Color Change and Control Technology for Oyster Protein Beverage,TS254.4
  20. Studies on the Drying Modle and Technology of Tilapia by Heat Pump,TS254.4
  21. Study on Deodorization Techniques of Oyster Protein Beverage,TS254.4

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Aquatic products processing industry > Basic science
© 2012 www.DissertationTopic.Net  Mobile