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Regulation of the M2Anti-inflammatory Kupffer Cells by IL-4and CD4~+CD25~+FoxP3~+Regulatory T Cells on Severe Acute Pancreatitis in Mice

Author: DengCaiHong
Tutor: LiuJun
School: Huazhong University of Science and Technology
Course: Department of Gastroenterology,
Keywords: Severeacutepancreatitis IL-4 Treg Kuffercells M2pylorization.
CLC: R576
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
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Abstract


Objective:To investigate the effective method of inducing severe acute pancreatitis with cerulein pluslipopolysaccharide (LPS) and the regulation of the M2anti-inflammatory kupffer cells by IL-4andCD4+CD25+FoxP3+regulatoryTcellsonsevereacutepancreatitisinmice.Methods:Normal group was induced by intraperitoneal injection of saline;model group was induced byintraperitonealinjectionofceruleinplusLPS.Modelgroupwasdividedintothreegroups:9h、12hand24hgroups after induction of SAP. Histopathological alterations of pancreatic tissues were evaluated amongthese three groups.2. Expressions of inflammatory cytokines mRNA in liver tissues were assessed byreal-timefluorescentquantitativereversetranscriptasepolymerasechainreaction(RT-PCR)betweennormalgroup and SAP8h+NS group. The mice of SAPmodels were divided into three groups in accordance withtheintravenousinjectionofthedifferentsolutions:SAPwithsalineinjectiongroup、SAPwithIL-4injectiongroup and SAPwith Treg injection group. Expression of IL-1β、TNF-α and IL-10mRNAin liver tissueswere assessed by RT-PCR;Expressions of CD163and CCR7in liver were assessed by confocalimmunofluorescencemicroscopy.Results:(1)TheresultsofHEstaining:pancreaticedema、inflammationandacinarcellnecrosisin24hgroupsafterinductionofSAP.(2)TheexpressionsofIL-1βandTNF-αmRNAinlivertissuesofSAP8h+NSgroupweresignificantlyhigherthanthoseofnormalgroup(P<0.1).(3)TheexpressionsofIL-1βmRNAinlivertissuesofSAP16h+TreggroupandSAP16h+IL-4groupweresignificantlylowerthanthoseofSAP16h+NSgroup(P<0.1));TheexpressionofIL-1βmRNAinlivertissuesofSAP16h+TreggroupwassignificantlylowerthanthoseofSAP16h+IL-4group(P<0.05);TheexpressionsofTNF-αmRNAinlivertissuesofSAP16h+TreggroupandSAP16h+IL-4group weresignificantlylowerthanthoseofSAP16h+NSgroup((P<0.1);TheexpressionofTNF-αmRNAinlivertissuesofSAP16h+TreggroupwassignificantlylowerthanthoseofSAP16h+IL-4group(P<0.1);TheexpressionofIL-10mRNAinlivertissuesofSAP16h+IL-4group wassignificantlyhigherthanthoseofSAP16h+NSgroupand SAP16h+Treggroup(P<0.1).Conclusion:It is a effective method using cerulein plus LPS to induce severe acute pancreatitis in mice. Kuffer cells arevery important in the development of SAP. IL-4and Treg promote the expression of the M2anti-inflammatorykupffercells,andthedown-regulationofTNF-α、IL-1βandCCR7andup-regulationofIL-10andCD163suggestIL-4andTreghavetherapeuticeffectsonSAP.

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Pancreatic diseases
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