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Obesity Intervention of Anthocyanins and Its Mechanism

Author: WuTao
Tutor: ZhengXiaoDong
School: Zhejiang University
Course: Of Food Science
Keywords: Anthocyanins obesity dietary intervention high fat diet mulberry sweet cherry blueberry honeysuckle
CLC: R589.2
Type: PhD thesis
Year: 2014
Downloads: 84
Quote: 0
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Abstract


Obesity is now acknowledged as a leading global health problem that causes detrimental effects. This condition is known to contribute to the risk of various chronic diseases such as type Ⅱ diabetes mellitus, coronary heart disease, hypertension, and several types of cancer. Currently available pharmaceuticals for obesity treatment have a number of limitations such as adverse effects and high rates of secondary failure. Therefore, natural products having anti-obesity effects are now being arranged into anti-obesity stategy. Anthocyanins are water-soluble plant polyphenolic pigments. Recently, these substances have attracted scientists’ interest because of their non-toxicity and healthy benefits, including anti-tumor, anti-inflammatory, reduce hematic lipids. etc. This study aimed to isolate and characterize anthocyanins from mulberry, sweet cherry, blueberry and honeysuckle; investigate their inhibitory influences on the profiferation and differentiation of3T3-L1proadipocytes; evaluate the effect of the different anthocyanin supplementation on high fat diet-induced obese animal model, including intervention and treatment experiment; study the mechanism of their effect on weight loss preliminarily. The results were as follows:(1) Mulberry juice and blueberry juice suppressed the body weight gain of high fat diet fed mice. Among the12weeks experiment perioed, intake mulberry or blueberry juice reduced body weight mice weight9.8%and7.3%respectively. Furthermore, both of juice supplementation could significantly reduce lipids accumulation in serum and liver tissue, improve insulin resistance, lower inflammatory cytokines gene expressions, such as IL-6, TNF-α. The polyphenols pigments, anthocyanins may play important part in the benefical function.(2) Anthocyanins from mulberry, sweet cherry, blueberry and honeysuckle have been isolated and characterized. There are three kinds of anthocyanin in mulberry anthocyanin, which are Cyanidin-3-glucoside, Cyanidin-3-rutinoside and Pelargonidin-3-glucoside. Sweet cherry anthocyanins mainly composed with Cyanidin-3-(2G-glucosylrutinoside), Cyanidin-3-rutinoside and Pelargonidin-3-rutinoside. The major anthocyanins in blueberry anthocyanins were Petunidin-3-arabinoside, Delphinidin-3-galactoside, Cyanidin-3-galactoside and Pelargonidin-3-glucoside. Honeysuckle anthocyanins composed with Cyanidin-3-glucoside, Cyanidin-3-rutinoside, Peonidin-3-glucoside and Pelargonidin-3-glucoside.(3) The results indicated that anthocyanins from mulberry, sweet cherry and blueberry could inhibit the profiferation and differentiation of3T3-L1proadipocytes, induce the cell apoptosis. Treated the preadipocytes with the three kinds anthocyanins100μg/mL for24h, the inhibiton ratio on preadipocytes proliferation was39.6%,40.2%and20.3%respectively. In3T3-L1preadipocytes differentiation exper iments, it was found that three kinds of anthocyanins at200μg/mL-1could inhibit preadipocyte differentiation by28.3%,30.7%and37.6%respectively.(4) Dietary supplementation with anthcyanins from mulberry, sweet cherry or blueberry suppressed the body weight gain of high fat diet fed mice. After the12weeks experiment, intake anthocyanins from mulberry at low dose or high dose reduced body weight gain by11.8%and21.4%respectively. Consumption sweet cherry anthocyanins at low dose or high dose prevented body weight gain by11.8%and21.4%respectively. Supplementation of blueberry anthocyanins at at low dose or high dose inhibited body weight gain by8.1%and12.6%respectively. The study exhibited that all the three sources of anthocyanins could impaired hepatic function, reduce the resistance to insulin, decrease the lipids accumulation in serum and liver tissue, etc.(5) In obesity treatment experiment, the effects of anthocyanins from mulberry, sweet cherry, blueberry and honeysuckle on wight loss were investigated. We found that anthocyanins does not change the trend of great body weight, but slow the body weigt gain. Cosumption anthocyanins from mulberry, sweet cherry, blueberry and honeysuckle could prvent body weight gain by32.7%,29.6%,19.4%,24.1%respectively. Furthermore, the obesity treatment experiment also confirmed a phenomenon that anthocyanins could reduce the accumulation of lipid in serum and liver function.(6) Anthocyanins supplementation significantly increase the heapatic SOD and GPx activity, up-regulation the experession of CPT-1genes, down-regulation the expression of FAS、PPARγ genes and inflammation factor genes, such as IL-6TNF-α、NF-κB、iNOS、IFNγ gene.(7) The mechanisms of anthocyanin exert anti-obestiy effects possibly associated with inhibition of fatty acid synthesis, increase the oxidation of fatty synthesis and decrease the production of inflammatory.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Metabolic diseases > Lipodystrophy
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