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Effect of pregnancy maternal high fat diet on the growth of young rats

Author: ChengPanGui
Tutor: ZhangZhiXin
School: Beijing University of Traditional Chinese Medicine
Course: Integrative Clinical Pediatrics
Keywords: growth retardation high-fat diet during early period neonatal offspring maternal mechanism
CLC: R723.14
Type: Master's thesis
Year: 2014
Downloads: 67
Quote: 0
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Abstract


Objective1. Observe the offspring growth exposed to the impact on the environment of maternal high-fat diet during early period (pre-pregnancy and pregnancy).2. Observe the fat deposit of the liver and the Secretion of IGF-I under high fat environment.3. Explore the signaling pathway of MAPK and PI3K/AKT-1in growth plate chondrocytes of long bones of infant rats.MethodsForty3-week-old female Sprague Dawley rats were randomly divided into two groups, high fat group (group HF) and control group (Group C). groups of HF20female rats fed a35%fat diet which60%kcal was attributed to fat, groups of C20female rats and24male rats fed standard chow which10%kcal was attributed to fat. After8weeks of feeding, eight female rats from each of dams were observed liver pathology, and the rest female rats were mated with male by the ratio of1:1, during pregnancy which were maintained on either high fat or chow feed. After birth,(1)Body weight was assessed by electronic scales, Body leight was assessed by caliper.(2) The blood was collected by decapitated, ALT, AST, TG, CHO, HDL, LDL, GLU were detecting by automatic biochemical analyzer. Plasma IGF-I assays were carried out using enzyme-linked immunosorbent assay kits.(3)The liver of offspring and maternal was sliced and stained with HE for pathological observation.(4) Immunohistochemical detection of insulin substrate (IRS1) and phosphorylation (Phospho-IRS1) was performed on paraffin embedded sections of offspring left leg tibia, femur.(5) Western blot detection the protein expression of IGF-1in neonatal hepatocytes and the signal path PI3K/Phospho-PI3K, AKT1/Phospho-AKT1, MAKP/Phospho-MAKP protein expression in neonatal left leg tibia, femur.ResultsNinety-five neonatal offspring produced in HF group,128neonatal in control group. The weight of HF group offspring were less than group C [(6.11±1.17) g VS (6.75±0.81) g, P<0.05], The leight were decreased than group C [(66.71±3.94) mm VS (76.9±3.76) mm, P<0.05]; Decreased by20.1%serum IGF-I of HF group compared with group C [(43.35±17.02) pg/ml VS (54.29±12.34) pg/ml, P>0.05]. AST was elevated in HF group than group C [(345.86±135.62) VS (225.89±47.64), P<0.05]. ALT, TG, CHO, HDL, LDL, GLU no significantly differences between the two groups (P>0.05);Liver Histopathology showed that HF group maternal and offspring have significant lipid deposition in liver, liver cells have more small, dense lipid deposition and cytoplasm transparent vacuole in the liver; Immunohistochemistry showed, IRS and Phospho-IRS on tibial cartilage cells of HF group were positive, No significant difference between two groups by the integrated optical density and average optical density; IGF-I protein expression was elevated in HF group [(1.45±0.33) VS (0.65±0.22), P<0.05], MAPK expressing higher in osteochondral cells of HF group [(1.13±0.12) Comparison VS (0.88±0.13), P<0.05], PI3K [(1.19±0.44) VS (0.83±0.26, P>0.05)], AKT1/Phospho-AKT1[(0.97±0.14VS1.18±0.44, P>0.05)] is no difference between two groups, Phospho-PI3K, Phospho-MAPK have not the corresponding protein bands were detected, which affected by technical factors.Conclusion:1. Early exposure to a high fat environment reduce the neonatal offspring body length and weight, lead to a intrauterine growth disorders.2. Exposure to a high-fat environment, the neonatal offspring liver lipid deposition significantly and impaired liver function. Serum IGF-I levels decreased, but the protein expression of IGF-I in liver cells were not reduced.3. Signaling pathways PI3K, AKT1after IGF-I receptor not damaged, and the total expression of MAPK signaling pathways display increased, the effectiveness of IGF-I did not reduce in chondrocytes. The mechanism of growth disorders induced by early exposure to the high fat environment still need for futher research.

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CLC: > Medicine, health > Pediatrics > Infant nutrition disorders > Digestion and nutrition disorders > Obesity
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