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Down-regulated miR-9Induced by Curcumin Inhibits Colon Cancer Cell Proliferation and Migration Research

Author: GongShaoXin
Tutor: ZhuangYingZhi
School: Nanhua University
Course: Oncology
Keywords: miR-9 colon cancer proliferation migration E-cadherin
CLC: R735.35
Type: Master's thesis
Year: 2012
Downloads: 2
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Abstract


Objective: To investigate the expression of the miR-9in colon cancer treated withcurcumin and its effects on cell proliferation as well as associated mechanisms.Methods: We evaluated the miR-9expression levels in two colon cancer ofgroups,Curcumin treated group and DMSO control group, by quantitation RT-PCR.The MTT and cell scratch showed Curcumin promoting the human colon cancerSW480cell’ growth and invasive ability through regulating miR-9.Western blotingshowed the protein expression of miR-9target gene E-cadherin.Results: The qRT-PCR showed the miR-9expression in curcumin treated group wassignificantly higher than DMSO control group, The difference was statisticallysignificant(P <0.01). MTT test showed the repression rate of colon cancer cell SW480handled with different concentration curcumin (1、5、10、20μmol/L) for24h was21.87%,29.34%,37.78%,44.81%,respectively, for48h was27.81%,39.62%,32.78%,54.31%, respectively, for72h was50.79%,58.38%,68.37%,72.35%, respectively. Itis proved that curcumin can inhibit the prolifiration of SW480in a time anddose-dependent manner. MTT assay showed that the cells transfected with miR-9inhibitors for12h,24h and48h, were inhibited significantly in a time-dependentmanner compared with the control group (P <0.05).The cell scratch test certified thatmiR-9inhibitors could inhibit the colon cancer cell motility and invasion. The woundhealing rates after transfected by miR-9inhibitors for24h and48h were0.375±0.012,0.556±0.026, respectively. It is showed that inhibitors could alleviate significantlythe SW480cell scratch wound healing compared with the control group which has arate0.803±0.008.Western bloting showed that the expressions of E-cadherin proteinwere inhibited by miR-9also.Conclusions:1. Curcumin inhibite the proliferation of colon cancer cell line SW480cell bydown-regulating the expression of level miR-9. 2. miR-9can promote colon cancer cell migration by down-regulating the expressionof E-cadherin.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Intestinal neoplasms > Colon tumor
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