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The Effect of Peroxisome Proliferator-activated Receptorsγ Agonist to Oxidative Stress and Islet Function of Obstructive Sleep Apnea-hypopnea Syndrome Model

Author: CaiZuoZuo
Tutor: ZhouYan
School: Guilin Medical College,
Course: Respiration within the science
Keywords: Intermittent hypoxia (IH) Ostructive sleep apnoea/hyopnoeasyndrome (OSAHS) Oxidative stress (OS) Islet function Pioglitazone
CLC: R766
Type: Master's thesis
Year: 2013
Downloads: 19
Quote: 0
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Abstract


Objective: To study the effect of peroxisome proliferator-activated receptors γagonist pioglitazone to oxidative stress and islet function of obstructive sleepapnea-hypopnea syndrome model. Methods: Twenty four male Sprague-Dawley(SD) rats were divided into3groups: blank control group (control group),intermittent hypoxia group (model group), pioglitazone intervention group(treatment group). Each group has eight rats.Model group and treatment grouprats were subjected to alternating cycles of hypoxia(6%一8%O2in N2for20—25s)and normoxia (2l%O2in N2for2min) every90s for8h per day.Ratsin the treatment group were treated with pioglitazone (10mg·kg-1·d-1) by gavaged,and those in the control group and model group were treated with equivalentdistilled water.After eight weeks,using method of ELISA assesses serumTNF-α、IL-8、8-iso-PGF2α、insulin; Using glucosinolates barbituric acidmethod assesses serum malondialdehyde; Using the colorimetric methodassesses serum GSH-Px; Using methods of glucose oxidase method assessserum fasting blood-glucose. Using HOMA calculates islet beta cell functionindex (HOMA-beta) of the rats, assessment of islet function. RT-PCR and western blot are used to detect NF-κ B of pancreatic tissue. Results: Comparedwith control group rats, model group and treatment group rats: islet βcellfunction index and GSH-Px decline, TNF-α、IL-8、MDA、8-iso-PGF2α ofserum and NF-κ B mRNA and protein of pancreas tissue express rise (P<0.05orP<0.01). Compared with model group rats, treatment group rats: isletβcellfunction index and GSH-Px rise, TNF-α、IL-8、MDA、8-iso-PGF2α of serumand NF-κ B mRNA and protein of pancreas tissue express decline (P<0.05orP<0.01). HOMA-β was negatively correlated with TNF-α、IL-8、MDA、8-iso-PGF2α content,and positively correlated with GSH-Px activities(P<0.05or P<0.01).Conclusions: OSAHS animal model had islet function impaired.Oxidative stress was involved in the occurrence of OSAHS. Oxidative stresscaused by intermittent hypoxia may be the important mechanism of OSAHSislet function damage. Peroxidase body growth activated receptor (PPAR)gamma agonist pioglitazone probably improved the function of islet beta cellmetabolism and secretion by regulating oxidizing substance content of islet betacells.

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