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Extraction、Purification and Determination of Resveratrol from Mulberry Resource

Author: ZhanPengFei
Tutor: ZhuXiangRui
School: Zhejiang University
Course: Special Economic Animal Feeding
Keywords: Resveratrol Mulberry High Performance Liquid Chromatography Macroporous resin
CLC: R284.1
Type: Master's thesis
Year: 2009
Downloads: 29
Quote: 0
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Abstract


Resveratrol as a natural polyphenolic compounds display a variety of biological activity , is a natural anti-tumor, anti- cardiovascular diseases , anti-mutagenic , anti-bacterial , anti-inflammatory , anti- oxidation , induction of apoptosis and estrogen regulation, and function of the material , is widely used in medicine and health and other fields . Resveratrol mainly from some Chinese herbal medicines , such as Polygonum cuspidatum extract , so tap other resources , and to study the extraction process is of great significance . The study found that resveratrol Sang resources exist . China Sang resource rich countries , so the researchers extracted from mulberry resources resveratrol angle from the use of resources and economic significance . In this thesis, the various parts of the mulberry extraction and identification of resveratrol trial to demonstrate the presence of resveratrol in some parts of the mulberry . Mori leather , mulberry root skin and mulberry content is relatively rich , organic solvent extraction experiments by single factor and orthogonal experiment to determine the optimum extraction conditions . When 80% ethanol as extraction solvent , in accordance with the ratio of solid to liquid 1:25 , extraction time 4h method to extract the best . Measured Sang extracted by this method in various parts of the resveratrol content (μg / g): Sang peel 7.9 ; , mulberry root Paper 5.3 ; mulberry stalk skin 3.2 ; mulberry leaves 1.6 ; Mulberry Juice 1.0 . Mulberry branch skin as raw materials , with 80% ethanol , solid to liquid ratio of 1:25 , extraction 4h, the resulting crude extract after macroporous resin purification test , after comparing different models of the five kinds of macroporous adsorption resin solution in ethanol gradient elution, screened out more suitable AB-8, DM130 purified resin , AB -8 is determined by the tracking and detection of the HPLC DM130 two resins combined with the optimized purification resveratrol process , and by HPLC the resveratrol content up to 91% , for the large-scale extraction and purification the resveratrol can learn information .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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