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Research on Surface Modification of RGD Peptides onto Decellarized Scaffolds to Promote Constuction of Tissue Engineering Heart Valve

Author: ZhouHuaSong
Tutor: ChenWenSheng;GuChunHu
School: Fourth Military Medical University
Course: Surgery
Keywords: RGD peptide. Tissue Engineering Heart valve Acellular valve Surface modification Cell Adhesion
CLC: R318.1
Type: Master's thesis
Year: 2008
Downloads: 41
Quote: 0
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Abstract


Most major treatment for heart valve disease valve replacement surgery. The clinical application of artificial heart valve, mechanical valve and bioprosthesis two categories, can improve and prolong the lives of patients, but did not reach the desired level. Mechanical valves require lifelong anticoagulation, embolism and bleeding rate; bioprosthesis degeneration, calcification. The same kind of valve is superior to xenograft bioprosthesis, but the source is limited, and there are also a decline. The research and development of tissue engineering heart valve is the current trend of the times. Use of decellularized valve tissue engineering heart valves, to have any polymer biodegradable material which can not be replaced by the advantages. However, due to the removal of a large number of extracellular soluble matrix the acellular scaffold materials process, including the regulation of cell adhesion molecules known. Construct tissue engineered heart valves using decellularized valve is how regulation to promote the seed cell adhesion, proliferation and ECM newborn, Seung important issues to be resolved. Learn biologically inert material by way of surface modification, can improve or increase the biocompatibility, the successful experience of the physical and chemical properties of the material, the surface modification proposed acellular flap, to improve their physical and chemical properties, and promote the seed cell adhesion, proliferation and differentiation. Bioactive molecules RGD peptide (arginine - glycine - aspartic acid) as the natural ECM important, the most widely and most effective to promote cell adhesion peptides. In this study, first of all through the application of our laboratory screening, trypsin legal system TritonX-100 prepared acellular porcine aortic stent the the cell valve scaffold materials processing go epichlorohydrin, crosslinking agents, and the laboratory screening - for further A comparative study. On this basis, the application of peptides (sequences for YGRGDSP Tyrosine - glycine - arginine - glycine - aspartic acid - serine - proline) and epichlorohydrin joint surface modification of acellular porcine aortic valve and handling change material cell adhesion. Acellular scaffold improved by crosslinking RGD surface modification preliminary attempt. Physical and chemical properties of the first part of the preparation of acellular porcine aortic valve and epichlorohydrin modified experiment. By trypsin digestion and hypertonic, hypotonic detergent TritonX-100 rinsing prepared acellular porcine aortic valve, GA, EC and GA EC (GE) methods were used to dealing with acellular porcine aortic valve, compare between mechanical, and microstructural stability, tissue compatibility of the difference. Results using trypsin method TritonX-100 cellular components of the porcine aortic valve can be completely removed. GA, EC and GE three processing methods can make APAV in mechanics, shrinkage temperature, in vitro anti-enzymatic capacity has been strengthened, and the where GE effects best. 3 animals subcutaneous, plasma protein and platelet adhesion experiment results show that, the EC process does not change the APAV organizational compatibility and blood compatibility, the EC GA group processing APAV significantly lower GA biotoxicity, histocompatibility, the hemocompatibility significantly superior to GA treated. The conclusion of this experiment suggested GE processing APAV, APAV can significantly improve the physical and chemical properties, reduce GA's biological toxicity, will help improve the tissue phase and blood compatibility. The second part of the the epichlorohydrin Joint RGD modified experimental acellular porcine aortic valve. Objective To study decellularized valve RGD peptide surface modification of cell adhesion. The method various acellular flap group with YGRGDSP peptide, reaction by a ninhydrin method, a fluorescent marker display method to compare the binding rate of each group. Planting rat aortic MFBs, precipitation method to detect cell adhesion rate. Results ninhydrin, each group comparison of the fluorescence imaging method display polypeptides may be crosslinked to a good EC, GE group, the optimal reaction conditions were: room temperature, 1.5mg/ml RGD, pH7.4, sustained oscillation 12h. The RGD peptide modification EC, the GE group cell adhesion rate was higher than that APAV improved significantly. Conclusion EC, the YGRGDSP peptide covalently grafted fixed acellular porcine aortic stent surface modified the EC joint RGD peptide surface modification decellularized valve can be significantly improved cell adhesion.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Artificial organs and organ
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