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The Identification of Trichophyton Rubrum and Trichophyton Mentagrophytes by PCR Fingerprinting

Author: ZhuHongMei
Tutor: LiaoWanQing;WenHai
School: Second Military Medical University
Course: Dermatology and Venereology
Keywords: Trichophyton rubrum Trichophyton mentagrophytes PCR fingerprinting Gene identification
CLC: R379.22
Type: Master's thesis
Year: 2000
Downloads: 81
Quote: 1
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Abstract


Trichophyton rubrum and Trichophyton mentagrophytes the superficial mycoses main pathogen colonies can was wool, fluff, powder, both traditional identification based primarily on training the morphological, physiological and biochemical methods, but the red hair rubrum colonies producing red phenomenon by medium type, pH, cultivation temperature and other factors affect the colony shape and Trichophyton mentagrophytes are very similar in lost production of red ability, some phenotypic Trichophyton rubrum strains will urea agar medium to discoloration; rather be ringworm Trichophyton some strains produce pigment resembles Trichophyton rubrum colonies back color. These phenomena to the traditional method of identifying the problem. Currently has some molecular biology methods, such as the internal transcribed spacer 1 (ITS1) sequencing, genomic DNA RFLP analysis, is used to distinguish between these two dermatophytes, but these methods are complicated to operate, the DNA quality and The amount of demanding, not suitable for clinical or research with a large sample. PCR method, repeat the Sequence (GACA) 4 (GTG) 5 and M13 center sequence (5'-GAGGGTGGCGGTTCT-3 oligonucleotide ') 3 primers amplified different regions, different phenotypes of Trichophyton rubrum and Trichophyton mentagrophytes clinical isolates DNA observed the PCR fingerprint differences and genotype with traditional phenotype relationship. The results found that the three kinds of primers are issued by the species-specific DNA fingerprint visible Trichophyton rubrum and Trichophyton mentagrophytes presented. These three primers were Trichophyton rubrum to be ringworm the Trichophyton identification with species-specific, but there is no intraspecific classification. With these three primers were also identified 2, according to the phenotypic identification Trichophyton mentagrophytes Trichophyton rubrum. Therefore, the authors believe, Trichophyton rubrum and be ringworm Trichophyton phenotypic variation can be through micro-satellite DNA primer PCR fingerprints were identified distinction. (GACA) 4 due to differences in the amplified bands most clearly, which is more worthy of the recommended primer. Identification of Trichophyton rubrum and Trichophyton mentagrophytes distinguish clinical differential diagnosis of superficial mycoses epidemiological investigation and prevention research has a very important significance.

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology ) > Pathogenic fungi ( molds) and actinomycetes > Skin of filamentous bacteria > Trichophyton
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