Dissertation > Excellent graduate degree dissertation topics show

Analysis of Infrared Spectral Features and Identification of Al-responsive Genes in Small Black Soybean with Aluminum Stress

Author: ZhangYaZuo
Tutor: LiKunZhi
School: Kunming University of Science and Technology
Course: Botany
Keywords: Fourier transform infrared spectroscopy (FTIR) suppression subtractive hybridization cDNA library Al-responsive genes small black soybean
CLC: S529
Type: Master's thesis
Year: 2011
Downloads: 7
Quote: 0
Read: Download Dissertation

Abstract


Currently, acidic soil accounts for 40% of the world arable lands. Aluminum (Al) toxicity is one of the major factors limiting the growth of plants in acidic soils. Along with the increase of population and the reduction of arable lands, improvement and utilization of acid soils have received much attention. Lime and alkaline fertilizer are used to reduce soil acidity in agriculture, but this method costs high and could not fundamentally solve problems. From the long-term benefits, application of genetic engineering means, breeding and utilization of crop resistance to aluminum is the most economic, effective and pollution-free fundamental measure. Yunnan is a productive region of small black soybeans. There is mainly red soil in Yunnan region, and soil acidification is serious increasingly by the influence of intensive management in modern agriculture. Therefore, it is necessary to study on aluminum detoxification mechanism in small black soybean. In this research, we explore aluminum detoxification mechanism of the small black soybeans through determining the physiological indexes, analysing the Fourier infrared spectral features and constructing the forward suppression subtractive hybridization (SSH) cDNA library of small black soyabean under aluminum stress. Main results were as follows:1. With the 50μmol·L-1 Al treatment, the relative root elongation of Yunnan small black soybeans were obviously higher than Medicago sativa. Root dyeing experiments also indicated that the Al tolerance of Yunnan small black soybeans was relatively stronger than Medicago sativa. The contents of soluble protein, hydrogen peroxide, soluble sugar and malondialdehyde were increased intensively for small black soybeans roots and leaves with the aluminum stress. There were fluctuation of the contents with different time treatment of aluminum stress, but some of them also have ascending trend. The activities of superoxide dismutase, catalase and peroxidase decline basically with the increase of aluminum stress and the extension of time. The activity of superoxide dismutase appears rising trend with the extension of aluminum stress time.2. The Fourier transform infrared spectroscopy (FTIR) technical analysis showed that there were differenttions among the FTIR spectraes of root, stem and leave of Yunna small black beans under different concentrations of aluminum stress. The characteristic peaks of sugar and methylation were increased with the increase of the concentration of aluminum. When the aluminium concentration increased to 100mol·L-1, Al toxicity was strengthened and the peak was declined. The change of peaks in roots stems and leaves of small black soybeans had different trends with different time treatment of aluminum stress. Under short time aluminum stimuli, the amount of organic compounds such as amino acid, carbohydrate, lipid, etc would be increased, which would play an important role in osmoregulation. With extension of the aluminum stimulation, synthesis and transportation of the organic compounds were hampered and the contents were declined in different degree.3. SSH method was adopted to construct a forward SSH-cDNA library for small black soybean, in this way, it is easy to analysize the genes induced by Al stress. Sequence analysis gets 159 ESTs, among which 102 ESTs are known function genes, including 26% related to metabolism,25% related to defense, stress and cell death,14% related to protein synthesis,13% related to signal transduction and transcription,8% related to transporter, 9% related to protein destination/storage,3% related to cell structure and cell growth, the least related to energy is about 2%. Gene unknown function needed to be further identified. The information obtained from the SSH library established an important foundation for the Al detoxification mechanism study in small black soybean at molecular level.4. Expression profiling of the 10 genes was analysed with RT-PCR, which confirmed that UDP-glucose 4-epimerase, Quinone oxidoreductase, Mn superoxide dismutase, Cu/Zn superoxide dismutase, p-type H+-ATPase, glutathione S-transferase, peroxisomal membrane protein, mitogen activated protein kinase, cytochrome P450, class I chitinase were up-regulated. This further illustrated the reliability of the library.

Related Dissertations

  1. The Construction of cDNA Libraries and Analysis of Differentially Expressed Genes at Maturation Stages of Ricinus Communis Embryo,S565.6
  2. Yunnan camellia East Camellia sasanqua FTIR study,S685.14
  3. Construction of Suppresstion Subtractive Library of Alfalfa as Influenced by Aluminum Stress,S541.9
  4. Construction of Subtracted cDNA Library by Suppression Subtracted Hybridization for Differentially Expressed Genes in Mycobacterium Tuberculosis,R392
  5. Cloning, Characterization of Ankylosing Spondylitis-related Genes in Homo Sapiens,R593.23
  6. Construction and Analysis of Suppression Subtractive Hybridization cDNA Library of Cucumber Induced by Fusarium Oxysporum,S642.2
  7. SSH cDNA Library Construction of Wheat with New Rust-resistant Gene and Preliminary Screening,S512.1
  8. Guizhou Xingyi Gangue’s Activation Research and the Experimental Exploration of the PAC Preparation,TD849.5
  9. Analysis of Differentially Expressed Genes at Maturation Stages of High and Low Oil Content Soybean Seed,S565.1
  10. Construction of a Stubtracted cDNA Library and Cloning of Differentially Expressed Genes in Stomach Signet Ring Cell Carcinoma,R735.2
  11. The Study of Molecular Biology on Drought-resistant Mechanism of Halonxylon Ammodendron (Mey.) Bge. Seedlings,S793
  12. Construction of Subtractive cDNA Library of Esophageal Cancer and Observation the Clinic Significance of ZNF14 Expressed in Esophageal Cancer,R735.1
  13. Construction of the Mantle cDNA Subtractive Library of Haliotis Discus Hannai Ino Fed with Vitamin E-deficiency Diet and Cloning of the Full Length of the Related Genes,S917.4
  14. Construction and Preliminarily Analysis of Suppression Subtracted cDNA Library from Drought Stressed Citrus Leaves,S666
  15. Identification of Differentially Expressed Genes in Lipopolysaccharide-stimulated Yellow Grouper Epinephelus Awoara Spleen and Expression of DAD1, AIF-1 and RGS16,S943
  16. Rhizoctonia solani infection of rice genetic diversity and early analysis of up-regulated genes,S435.111.4
  17. Rat short interval successive partial hepatectomy liver regeneration upregulated genes Cloning and Functional Analysis,R346
  18. Difference Expression Gene Cloning, Screening and Identifying Related to the Dermal Wound Healing,R602
  19. Study on Molecular Mechanism of the Effect of Liver Cirrhosis on Hepatocellular Carcinoma Carcinogenesis,R735.7
  20. The Study of Atherosclerosis and the Filter of Different Expression Gene in Intermittent Hypoxia State,R543.5

CLC: > Agricultural Sciences > Crop > Legume crops > Other
© 2012 www.DissertationTopic.Net  Mobile