Dissertation > Excellent graduate degree dissertation topics show

Interleukin-9 and Interferon-gamma Are Contributing to Airway Inflammation in Rat Model of Chronic Obstructive Pulmonary Disease

Author: YangXueMei
Tutor: WanYiXin;ZhangQi
School: Lanzhou University
Course: Internal Medicine
Keywords: Chronic obstructive pulmonary disease Interleukin-9 Interferon-gamma Airway inflammation
CLC: R563.9
Type: Master's thesis
Year: 2006
Downloads: 148
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigate the changes of interleukin9(IL-9) and interferon-gamma(IFN-γ) in smoke-induced of chronic obstructive pulmonary disease(COPD) rat models.Methods Rat model of chronic obstructive pulmonary disease(COPD)was established by exposure Male Wistar rats to cigarette smoke daily for 75 days. Total cell counts and macrophages counts in BALF were examined. The level of IL-9 and IFN- Y in serum or BALF and lung tissue were measured with ELISA. Lung tissues section stained by HE was observed to study the morphological alternations and Lm and MAN were measured.Result (1) Lm in COPD group were higher than those in control group (P<0. 01) while MAN was decreased on the contrary. (2)Statistic analysis showed that level of IL-9 in serum or BALF and lung tissues of COPD group were higher than those ofcontrol group[ (4.02 + 2. 12pg/ml) VS (1. 12 + 0. 73 pg/ml), P<0. 01], [ (5.73 + 2.60 pg/ml) VS (0.97 + 0.45 pg/ml) ,P<0.01]> [ (24.92 +6.65 pg/ml) VS (11.42+4.23 pg/ml) ,P<0. 01], while that level of IFN-y in serum and in lung tissues were lower than those of control group[ (21. 54+7. 65)VS[ (69. 59 + 13. 68 pg/ml), P<0. 01] > [(21.88+6.11 pg/ml)VS (69.84 + 15.35 pg/ml), P<0.01]. (3)In the BALF of COPD group, total cell counts was (5. 29 + 1. 61) 108/L , macrophages counts was (4. 42 + 1. 38) X10 /L, while in the BALF of control group , they were (2.10 + 1.19) X108/L, (1.84+0.93) Xo10 /L, respectively. There were significances differences in these indexes between the smoke exposed group and the control group (P<0.01). (4) Positive correlations were demonstrated between the level of IL-9 in BALF or in Lung tissue and macrophages counts in BALF in COPD group (P<0.01 or P<0. 05), but not the level of IL-9 in serum. And there were negtive correlations between the level of IL-9 and IFN- y in Lung tissue.Conlusions ( 1 ) COPD rat model were successfully established by passive cigarette smoking;Cigarette smoke exposure increased IL~9 and decreased IFN- y, this may play a role in smoke-induced emphysema. (2)Our data implied a close correlation between IL-9 in BALF and airway inflammation in COPDrat model. IL-9 may be used a diagnostic marker in airway-inflammation of COPD. (3) IL-9 play a role in COPD partly by restraining IFN-Y.

Related Dissertations

  1. The Influence in Different Route of Medication with Vitamin A on CD4~+CD25~+ Regulatory T Cell and Cell Factor of IL-4、 IFN-γ in a Rat Persistent Asthma Model,R562.25
  2. Observastion on Histomorphology, Ultrastructure and Expression of TNF-α, IL-1β of Unbalance of Dynamic and Static Forces Cervical Degeneration Disc in Rat Model Abstract,R681.55
  3. The Effect of Salmeterol/fluticasone on the BODE Index of COPD Patients,R563.9
  4. The Analysis of Nocturnal Blood Oxygen Fluctuation in Patients with Chronic Obstructive Pulmonary Disease,R563.9
  5. The Experimental Research of RNAi Technology Inhibiting Respiratory Syncytial Virus,R725.6
  6. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  7. Association of Interleukin-6 Gene Polymorphisms with Susceptibility to Coronary Heart Disease in Asian Populations: a Meta-analysis,R541.4
  8. Relationship Between Chronic Obstructive Pulmonary Disease and Coronary Atherosclerotic,R563.9
  9. The Espression of C-reactive Protein in Rats of the Chronic Obstructive Pulmonary Disease with Diabetic,R563.9
  10. Changes of Immunologic Function in Patients with Chronic Obstructive Pulmonary Disease and the Intervention by BCG Polysaccharides Nucleic Acid,R563.9
  11. The Impact of Anemia in COPD and Study on Hepcidin、IL-6 and sTfR in Patients with COPD,R563.9
  12. 100 Cases of COPD Patients with CAT Score Correlation with MMRC Score,R563.9
  13. Urotensin Ⅱ Receptor Mediates the Expression of Interleukin-6 in Rat Adventitial Fibroblasts Stimulated by Homocysteine,R543.5
  14. The Experimental Study about Protective Effects of Puerarin on Liver of Brain-dead Rats,R285
  15. Study of the Correlation Between Plasma Hcy, Hs-CRP, IL -6 and Vascular Cognitive Impairment No Dementia after Cerebral Infarction,R749.1
  16. The Influence of MK2 and IL-6 Expression after β-amyloid Injection into Hippocampus of Rats,R749.16
  17. Effects of A-SAA on the Expressions of Inflammatory Cytokines mRNA , Protein and Related Kinases of 3T3-L1 Adipocytes,R587.1
  18. Recrultment of MSCs to the Lung of Mice with Allerglc Asthma and the Mechanism,R562.25
  19. The Construction of Plasmids Containing IBDV VP2/4/3 and ChIL-18 Fusion and Co-expression Genes as Well as the Study on Their Immunogenicity,S852.5
  20. Detection and Clinical Significance of IL-18 and NSE in Serum of Newborns with Hypoxic-ischemic Encephalopathy,R722.1
  21. Research Advances of Exhaled Nitric Oxide in Clinical Applicatin of Asthma,R562.25

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Other
© 2012 www.DissertationTopic.Net  Mobile