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Preliminary Experimental Studies and Its Applications of Leaky Surface Acoustic Wave Biosensor Detection System

Author: WangYunXia
Tutor: FuWeiLing
School: Third Military Medical University
Course: Clinical Laboratory Science
Keywords: Leaky Surface Acoustic Wave sensor delay line nucleic acid hybridization phase frequency delay time Scanning Tunnel Microsopy
CLC: TH789
Type: Master's thesis
Year: 2006
Downloads: 84
Quote: 0
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Abstract


Objectives:1. To build a dual delayed linear style of leaky surface acoustic wave (LSAW) biosensor including detection and reference sensor in one chip. Three detecting methods including phase, frequency and delayed time were used and compared with each other.2. To explore and optimize the experimental conditions, such as ionic concentration and pH value, which markedly influenced the detection system of the LSAW biosensor in liquid phase.3. Using scanning tunnel microscopy (STM) to observe microcosmic change between biomolecule and gold particle on the surface of LSAW biosensor during the process of probe immobilization and hybridization, also the naked gold membrane. 4. To construct the LSAW biosensor detection system in liquid phase through a series of experiments.Methods:1. Technology of fine processing was firstly used to make the LSAW biosensor with dual delay linear style. Then we developed the dual channels resonance circuit and used the LabView software to record and analyze phase/frequency automatically. So the detection system of LSAW biosensor was constructed by combing all these components with network analyzer and computer.2. The consistence of reaction of dual channels sensor to the bio-reaction was measured with two indexes: phase and frequency.3. To explore the effects of the different ionic concentrations and pH values on the detection system of the LSAW biosensor and evaluate the consistency of the two channels corresponding to the outer reaction.4. HPV probe was immobilized on the gold electrode surface of LSAW biosensor by

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