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Establishment of Analysis of Gene Mutation of Ribonuclease Inhibitor in Blood Cells and Detection in Patients with Cervical Carcinoma

Author: DuanYing
Tutor: ChenMingSheng
School: Dalian Medical University
Course: Clinical Laboratory Science
Keywords: Ribonuclease Inhibitor Gene SSCP Gene Mutation Establishment of Analysis
CLC: R737.33
Type: Master's thesis
Year: 2006
Downloads: 30
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Abstract


Ribonuclease Inhibitor(RI) is a 50KD acidic protein existing in cytoplasm, which lies in all tissues and organs of the mammals extensively, especially affluent in the placenta of humans. RI can decrease the RNA degradation by RNase, because RI can inhibit the activity of RNase for the combination with alkaline RNase. Angiogenin(Ang) is a single strand 14 KD alkaline protein, belonging to RNase superfamily, and which can induce the formation of newly formed vascular net. The growth of tumor depends on the formation of the newly formed circumambient vascular, they can supply the necessary nourishment for the growth of tumor. There is 35% homology between the amino acid residue of the Ang and RNase and their similar spatial structure can both combine with RI as ligand. The dissociation constant of the RI-RNase complex was higher than the RI-Ang complex by 60 times, and RI can inhibit the formation of the newly formed vascular effectively. The objective of the paper was to establish a effective method for analysis of gene mutation of Ribonuclease Inhibitor(RNH) in the blood cells of patients with cervical carcinoma, what’s more, the RNH of 30 normal persons and 18 patients with cervical carcinoma were detected by means of the method established in the experiment. The RNH lies in the galianconism of the No.11 chromosomes(11P15.5), the whole length of the gene is 12310bp, including in 11 extron and 10 intron. The target gene fragments must contain the whole sequence of extron when primers were designed, and several target gene fragments contained two adjacent extron and parts of the adjoining introns, and there were 11 pairs of such primers. But the longer intron were divided into several fragments eligibly for amplification, and all the target gene fragments were less than 1000 bp, and there were 10 pairs of such primers, so there were 21 pairs of primers in the experiment, and most of the target gene

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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