Dissertation > Excellent graduate degree dissertation topics show

The Investigation of Cytotoxic Effect Induced by Peripheral Blood Mononuclear Cells in the Patients with Psoriasis Vulgaris

Author: WangLiLi
Tutor: ZhangShiFa
School: Dalian Medical University
Course: Dermatology and Venereology
Keywords: secreted TNFαand mebrane associated TNFα Psoriasis vulgaris Cytotoxic effect Lipopolysaccharide
CLC: R758.63
Type: Master's thesis
Year: 2006
Downloads: 34
Quote: 0
Read: Download Dissertation

Abstract


Objective: Tumour necrosis factor (TNF) as a cytokine with many kinds of biological activities in vivo, has functions of killing tumour cells or inhibiting their growth, promoting inflammation and adjusting immunity. The biological activities of TNF are mainly showed by tumour necrosis factor-alpha (TNFα) which is classic TNF. According to the difference of its existence forms, TNFαhas been distinguished with secreted TNFα(sTNFα) and mebrane associated TNFα(MA-TNFα). Psoriasis (Ps) is a kind of common sickness with chronic and inflam characteristics. Since the Goerdt’s finding of TNFαinducing endothelial cells activation in Ps vulgaris patients, more and more researches manifest the associativities between TNFαand psoriasis vulgaris. Our experiment is to investigate cytotoxic effect in L929 (mice fibroblasts) target cells by two forms of TNFαfrom peripheral blood monouclear cells (PBMC) in Ps vulgaris patients, and further illuminate the pathogenesy of sTNFαand MA-TNFαin Ps.Methods: We obtain monocytes from peripheral blood applying the methods of Ficoll density gradient centrifugation and adherency to 96-well plates, then prepare two type of TNFα――sTNFαand MA-TNFαusing different treatment with lipopolysaccharide (LPS) and acid buffer (AB). After culturing L929 with sTNFαor MA-TNFαfor 48 hours, we detect 570nm optical density (OD) to analyze the cytotoxic effect mediated by these two forms of TNFαin Ps patients and normals.

Related Dissertations

  1. Studies on Dynamic Regularity of Antibody Against G. Anatis and Development of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against G. Anatis Lps,S858.32
  2. Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats,R285.5
  3. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  4. Effect of Curcumin on Serum Levels of IL-18 in Rats Sepsis Induced by LPS,R285.5
  5. HuaYuxiaobi Decoction Taken Orally with the Cutem and Calcipotriol in Treating Plaque Psoriasis Vulgaris and Detection of TNF-α and IL-8,R285
  6. Clinical Research on the Treatment of psoriasis vulgaris from the blood,R275.9
  7. Preliminary Study on Out Membrane Structure and Immunogenicity Changes of Drug-resistant Aeromonas Hydrophila,S941
  8. Study of Injury of Human Dental Pulp Cells Mediated by Lipopolysaccharide and Protective Effects of Baicalin,R285.5
  9. Endophytic Fungi from Scapania Verrucosa Heeg. and Their Bioactive Metabolites,Q946
  10. Cloning, Expression of Bovine Souble CD14 Gene and Protection Against LPS Toxicity,S852.5
  11. The Clinical Study on Efficacy about Qu Feng Xiao Bi Tang Decoction in the Treatment of Static Psoriasis Vulgaris,R275.9
  12. Study on Outer Membrane Proteins and Lipopolysaccrides of Vibrio Harveyi,S941
  13. Preparation of Sinapyl Alcohol Derivatives & Neolignans and Evaluation of Their Cytotoxic Activities,R914
  14. Eukaryotic Expression of the Extracellular Domain of Human TLR4 and Its Effect on Inflammation Induced by LPS,R362
  15. Influence of LPS on Contractility of Pulmonary Artery Smooth Muscle Cells and It’s Mechanisms,R363
  16. Effects and Mechannisms of Annexin-1 on Macrophages in Mice,R363
  17. Endotoxemia enterogenic role in the pathogenesis of Alzheimer's disease and related mechanisms,R749.1
  18. The Role of SIGIRR in LPS-induced Ali in Mice and the Screening and Preliminary Study of SIGIRR Interacting Proteins,R563.8
  19. Construction of SSH cDNA Library, Characterization and Function of Two Important Physiological Molecules(BPI、DP1) in Large Yellow Croaker(Pseudosciaena Crocea),S917.4
  20. Development and Primary Application of Hybridoma Cell Lines Respectively Secreting Monoclonal Antibodies Against Infectious Bursal Disease Virus and Lipopolysa Ccharide of Shigellae in the Chichen,S854.43
  21. Experimental Research of Biological Effect on Anti-lipopolysaccharide Fab’,R644

CLC: > Medicine, health > Dermatology and Venereology > Dermatology > The reason is unknown skin disease > Psoriasis (psoriasis)
© 2012 www.DissertationTopic.Net  Mobile