Dissertation > Excellent graduate degree dissertation topics show

Cloning of ABC Transporter Genes from Saccharomyce Cerevisiae and Application for Phytoremediation of Heavy Metals

Author: LiangShuCai
Tutor: ChenShiYun
School: Wuhan Institute of Virology
Course: Biochemistry and Molecular Biology
Keywords: Phytoremediation transgenic plants Saccharomyce cerevisiae ABC transporters heavy metals
CLC: X173
Type: Master's thesis
Year: 2006
Downloads: 207
Quote: 0
Read: Download Dissertation

Abstract


Environmental pollutions are serious problems facing the whole world and sustainable development. The development of remediation technology for scavenging environmental pollution has become an important research field in recent years. As an environmentally friendly and low-cost remediation technolgy, phytoremediation has gained more and more attention, and phytoremediation via transgenic plants has become a hot spot. Studies have demonstrated that expression of metallothioneins (MTs), phytochelatins (PCs), glutathione (GSH) and transporters in plants can increase the accumulation and/or tolerance to heavy metals. Saccharomyces cerevisiae ABC transporters are a large family and could transport and detoxify the heavy metals. One of the transporters, YCF1, when expressed in plants, has shown to enhance the tolerance and accumulation of some heavy metals such as cadmium, lead, etc. However, the fuctions of other types of the ABC transporters of Saccharomyces cerevisiae need to be elucidated in plants.In this thesis, ten ABC transporter genes were cloned from Saccharomyces cerevisiae, and the plant binary expression vectors were then constructed.The purpose of this study was aimed at the functional comparison of these transporter genes in plants to find efficient transporter genes for heavy metal transport and accumulation. Transgenic tobacco plants expressing these transporters were obtained through the Argobacterium-mediated transformation.The results showed that YCF1 transgene was integrated into tobacco genome, but did not show tolerance toward heavy metals. T0 transgenic plants of some other transporter genes were tested through leaf disc assay, it was demonstrated that some transgenic lines showed increased tolerance toward Cd、Pb、Hg , The molecular analyses, the mechanisms of heavy metal tolerance and the genetic stability of these transgenic lines needs to be further elucidated.

Related Dissertations

  1. Uptake and Accumulation of Heavy Metals of Pistia Stratiotes and Eicharnia Crassipes under Combinated Pollution Condition,X173
  2. Study on the Synthesis and Adsorption Behaviour of Chitosan Derivative,TS254.9
  3. Risk Analysis and Mitigation Experiment of Heavy Metal Pollution in Croplands in Vicinity of Pollution Sources,X820.4
  4. Adsortions of Dye by Modified Activated Carbon and Heavy Metals by Rice Husk-Based Activated Carbon,X703
  5. Process Selenuum Enriched Flos Chrysanthemi and Its Antioxidative Activity,S682.11
  6. Bioremediation of petroleum hydrocarbon contaminated soil,X53
  7. Study on the Leachability and Treatment of Trace Elements in the Fly Ash,X773
  8. Study on Efficient Regeneration System and Agrobacterium-Mdiated Transformation of TPS1 Gene by Agrobacterium into Chrysanthemum Lavandulifolium,S682.11
  9. Stress Tolerance of Koelreuteria Paniculata to Polluted Soils of Manganese Mining Wasteland,X53
  10. Health Risk Assessment of Heavy Metals in Groundwater in Jinghuiqu Irrigation District,X824
  11. Nitrogen and Phosphorus Removal by Immobilized Chlorella Comparative Research as Well as Heavy Metals and a Variety of Nutrients on the Growth of Chlorella,X703.1
  12. Gene Expression and Functional Identification of Alcohol Acyltransferase in Melon Fruit,S652
  13. Research on Safety Evaluation of Corn Silk (Zea Mays L.),S567.219
  14. Adsorption of Heavy Metals by Carbonate Hydroxylapatite,X703
  15. Studies on the Transgenic Tobacco and Tomato with Quorum-quenching Gene from Ralstonia Solanacearu,S436.412
  16. Study on the Characteristics of Natural Restoration in Plants Growing on Mine Waste Platform,Q948
  17. Study on the Accumulation of Heavy Metals on Red Soil Upland and Environmental Assessment from Pig Manure in Intensive Breeding Farm,X53
  18. Study on Phytoremediation of Heavy Metal Pollution in Soil,X53
  19. Study on Preparation of Granulated Composite Materials of Montmorillonite for Treatment of Heavy Metal Wastewater,X703
  20. Research on Solidified Behavior of Heavy Metals in Clinker and Cement Product,TQ172.1
  21. Studies on Resistance to Potato Virus Y Through dsRNA-mediated Silencing Suppressor,S435.32

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental botany
© 2012 www.DissertationTopic.Net  Mobile