Dissertation > Excellent graduate degree dissertation topics show

Experimental Research of Influence of Nifedipine on Human Sperm Function in Vitro

Author: LiuXiJun
Tutor: XuJiXiu
School: Shanxi Medical
Course: Urology
Keywords: acrosome reaction nifedipine sperm hypo-osmotic swelling test calcium
CLC: R96
Type: Master's thesis
Year: 2006
Downloads: 71
Quote: 0
Read: Download Dissertation


Objective: To investigate the effect of Nifedipine (NIF) of therapeutic dosages on human sperm motility function , acrosome reaction , plasma membrane functional Integrity and osmosensitive calcium influx in vitro.Methods: Sperm were aseptically obtained by masturbation and prepared by swim-up technique to produce a spermatozoa solution of high motility from 31 healthy fertile men.The spermatozoa solution was incubated with the different concentrations of Nifedipine at 37℃in vitro. Motive parameters were measured by computer-aided sperm analysis (CASA). Measurements were carried out at 10 , 30 and 60 min in 11specimens , including Sperm motility and Sperm viability. The hypo-osmotic swelling (HOS) test was used to assess the sperm function. The FITC-PSA was used to stain the capacitated spermatozoa that have be incubated with the different concentrations of Nifedipine for 60 min in vitro.Acrosome reaction induced by progesterone was measured by means of a flow cytometer. The value of FITC fluorescence was accumulated and calculated by a special computer as the stained sample passed the flow cytometer. Intracellular calcium concentration was measured utilizing the fluorescent probe fura-2/AM before and after sperm medium dilution in distilled water.Results: The spermatozoa incubated with Nifedipine of therapeutic dosages in vitro showed significantly lower Sperm motility , Sperm viability and induced acrosome reaction rate than the control groups(p<0.01). Moreover the effect become more significant as the concentration increased. The spermatozoa incubated with Nifedipine of therapeutic dosages in vitro showed significantly lower HOS test scores than the control groups (p<0.01). Moreover they showed a reduced rise in intracellular calcium concentrations after hypo-osmotic challenge.Conclusion: Nifedipine of therapeutic dosages can inhibit human sperm motility function and acrosome reaction in vitro. They also can modify plasma membrane functional integrity and inhibit osmosensitive calcium influx. Nifedipine can result in male infertility in vitro. The results provide us background for studying whether Nifedipine can be used as a contraceptive which is placed in vagina before coitus.

Related Dissertations

  1. Study of Simultaneous Desulfurization and Denitrification with Calcic-Based Absorbent in a Fixed Bed,X701.3
  2. Phosphogypsum to restore the system vulcanizing process optimization and research of calcium,X786
  3. Study on the Procees for the Preparation of Prohexadione and Its Analogs,TQ452
  4. Preliminary Study of Os02g0198200, Which Connected with Lateral Root Development and Tillering of Rice,S511
  5. Kinase Activities Analysis and Localization of ZmCCaMK Induced by Abscisic Acid in Leaves of Maize Plants,S513
  6. The Physiological Mechanism of Improvement in Watermelon Salt Tolerance by Calcium and Its Effects on Fruit Quality,S651
  7. Effects of Calcium Stress on Physiological and Cell Ultrastructural Features in ’Atago’ and ’Kisui’ Pear Seedling,S661.2
  8. The Effect of High-level Copper and Zinc Addition on the Performance, Digestibilities and Gastrointestinal Enzyme Activities of Weaned Piglets,S828.5
  9. Effects of Long-term Located Fertilization on Forms Distribution of Soil Calcium in Protected Field,S158
  10. Blue-light Induced Changes of L-type Calcium Channel Subunit mRNA Expression and Free Calcium Ion of Human Retinal Pigment Epithelium Cells in Vitro,R774.1
  11. Preparation of Casein Phosphopeptides and Studies on Its Calcium-holding Ability,TQ936.16
  12. Permeating Calcium Fresh-Keeping Technologies and Production Quality Control of Deepfreeze Strawberry,TS255.4
  13. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  14. Study on Knowledge, Attitude, and Behavior to Calcium Nutrition Among Patients with Hypertension in a Community,R473.2
  15. The Expression Mechanism of Calmodulin in Rat with Brain Contusion,R651.1
  16. Cross-sectional Screening on Chronic Kidney Disease-mineral and Bone Disorder in Maintenance Hemodialysis (MHD) Patients in a Single Center,R692.5
  17. Roles of Small Conductance Calcium-activated Potassium Channels in Mice with Alzheimer’s Disease,R749.16
  18. Experimental Study of Effects of Calcium on Steam Gasification of Shenhua Lignite,TQ541
  19. Study on the Effect of Calcium Sulfate on Coal Ash Fusion Temperature,TQ533
  20. Study of Propylene Oxide Wastewater Pretreatment by Five-effect Evaporation Process,X78
  21. Asthmatic Serum and Cytokines Induced Down-regulation of FKBP12.6 Expression in Airway Smooth Muscle Cell and Its Role in Stress Induced Ca2+ Release,R562.25

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile