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Objective To investigate the expressions of PTEN, MGMT and DNA-PKcs in gynecomastia, and to investigate the expression of them in different age groups of gynecomastia and different histology types difrerently, and to analyze the relationship between them. The purpose of our study was to approach the significance of PTEN, MGMT and DNA-PKcs in proliferation of mammary gland of gynecomastia, and to study and research the pathomechanism of gynecomastia.Methods 68 consecutive cases of mammary gland of gynecomastia which were surgically excised or biopsy specimen in clinic service or paraffin block in pathobiology department from Qi Lu Hospital of Shangdong University and the The Second Hospital of Shandong University were studied. 24 normal comparison samples were mammary gland of normal man. The selected examples were divided into three diffrent histological types: constrictive type, constrictive type and prosperous and intense type. At the same time, the cases were divided into three diffrent age groups: middle-aged group, age-groupand young group. Immunohistochemistry was used to determine the expression of PTEN, MGMT and DNA-PKcs. The positive cells were counted with optical microscope under high power, and the data was analyzed by the x~2 test and Ridit analysis by using SPSS11.5 software for windows.Results PTEN, MGMT and DNA-PKcs were expressed in both gynecomastia and normal male breasts. PTEN was maily expressed in nucleus of ductal cellula epithelialis, MGMT in nucleus or endochylema of ductal cellula epithelialis, and DNA-PKcs in nucleus of ductal cellula epithelialis with more positive cell rate in more epithelial proliferation. In 68 cases, the positive expression rates of PTEN,MGMT and DNA-PKcs were 70%, 49% and 66% differently, the higher
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