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The Effects on NGF Expression after Coadministration of Bone Marrow Cells with bcl-2 to Cerebral I/R Injured Rats Via Internal Carotid Artery

Author: LiuTianMing
Tutor: HeZhiYi
School: China Medical University
Course: Neurology
Keywords: Internal carotid artery Bone marrow stromal cells bcl-2 gene Ischemia Reperfusion Apoptosis Nerve growth factor
CLC: R743.3
Type: Master's thesis
Year: 2006
Downloads: 58
Quote: 0
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Abstract


NGF expression in the bone marrow stromal cells with the bcl-2 gene therapy after cerebral infarction study Preface ischemic cerebrovascular disease and more serious threat to human health and life , has become today the third largest cause of death in the first disability cause , but still lack effective treatment . Damage to nerve cells and tissue repair and functional reconstruction has been an important issue for neuroscience research . The bone marrow stromal cells (bone marrow stromM cells, BMSCs) is the source of the nerve graft BMSCs as nerve cells can secrete a variety of nutritional factors , such as NGF , GDNF . Many experiments show that BMSCs an important role in tissue repair and reconstruction of nerve cell injury . bcl-2 gene is one of the most important regulatory genes in the process of apoptosis , it is also able to improve neurological function . Nerve growth factor is one of the most important biologically active molecules of the nervous system , nerve growth factor conducive to the repair of damaged tissue , in the case of different causes brain damage , can lead to an increase in the expression of NGF . Yi Li, Chen and other bone marrow stromal cells in combination with anti-apoptotic role of caspase inhibitor Z- VAD treatment of rats with focal cerebral infarction , that combination therapy can improve the survival rate of BMSCs and improve nerve function . Combination of bone marrow stromal cells with the anti-apoptotic gene bcl-2 to treat rats with focal cerebral infarction was observed whether the two can be the same synergistic effect of simultaneous detection of a neuroprotective effect of nerve growth factor (NGF) expression Do you want to increase . Materials and methods 40 adult male Wistar rats were randomly divided into four groups: control group , bcl-2 group , BMSCs group and BMSCs group of bcl -2 , n = 10 . Each group was subdivided in accordance with ischemia / reperfusion after 3 days, 14 days , divided into two subgroups ( n = 5 ) . All rats were made ​​of the model of middle cerebral artery reperfusion 2 hours . group of bcl-2 and BMSCs bcl - 2 rats at 3 hours after reperfusion , the right common carotid artery was slowly injected pLXSN- bcl- 2 plasmid . BMSCs group and BMSCs bcl - 2

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CLC: > Medicine, health > Neurology and psychiatry > Neurology > Cerebrovascular disease > Acute cerebrovascular disease ( stroke)
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