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In this paper, nutrient solution culture methods to seedling apparent symptoms of reference indicators produced by Agrobacterium T1 generation mutants were screened , and the mutant phenotype nitrogen nutrition pedigrees molecular detection of mutations were isolated Nitrogen pedigree further from nitrogen nutrition characteristics and root characteristics were validation studies . The main results are as follows: one through the flowers 11T-DNA insertion mutants of 2173 T1 generation pedigrees nutrient solution culture screening of mutations observed in pedigree 152 , accounting for 6.99% screening population . Mutant types are divided into five categories, namely nitrogen auxotrophic mutant , mutant roots , albino type , etiolated seedlings type dwarf plant type, the mutation rate was 1.24% , 0.33% , 4.23% , 0.87% , 0.33 percent . 2 PCR testing showed that mutations in 27 pedigrees Nitrogen has 14 families were separated . Nitrogen expressed as mutant pedigree : small plant, the leaves are small , yellowish green , hair roots slow, poor fine roots and root hairs , root more yellow , white and poor root development . 3 through 12 consists of home -based T1 -and T2 generation of different nitrogen concentrations culture results showed that 955 lines at different nitrogen levels T1 -and T2 represents now the same separation characteristics , indicating that the mutation and T-DNA insertion directly related , through genetic analysis and inspection , found that mutations in the T1 generation pedigree 955 T2 generation segregation ratio of 3:1 are the inheritance of 955 tied with a mutated gene and showed recessive mutations. 4 through 955 T2 generation plants root system characteristics and differences in nitrogen metabolism studies have shown that : 955 line mutations in plants as ammonium nitrate absorption and uptake rates were reduced , the plants to reduce nitrogen content , nitrate reductase activity decreased, plant nitrogen assimilation capacity decreased, thus affecting root growth and development , the performance of shorter roots , root volume and root active area smaller , plant height and chlorophyll SPAD value decreases.
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