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Study on Phytosulfonine, a New Plant Peptide Hormone

Author: YangYanHui
Tutor: ChenXinJian
School: Henan Agricultural University
Course: Crop Genetics and Breeding
Keywords: The plant sulfo peptide hormone Precursors genes PSK-α Tobacco BY-2 cells Proliferation Bioanalytical Separate Appraisal
CLC: S482.8
Type: Master's thesis
Year: 2006
Downloads: 83
Quote: 1
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Abstract


The plant sulfo peptide of prime (phytosulfokine, abbreviated PSK) peptide discovered in recent years, a new type of plant growth regulator substances. It is two short peptide substance - tetrapeptide sulfonated (PSK-β) or pentapeptide (PSK-alpha), and its structure is [H-Tyr (SO 3 H)-Ile -Tyr (SO 3 H)-Ile-Tyr (SO 3 3 H)-Thr-OH] or [H-Tyr (SO H)-Thr-Gln-OH]. In 1996, Japanese scholars Matsubyashi and other asparagus culture, cell culture medium conditioned medium (conditioned medium, CM) found PSK, which can promote the growth and proliferation of cells. PSK research is limited to Japanese scholars Matsubayashi a laboratory, as a plant growth regulator substances in urgent need of in-depth and extensive research. This study from a preliminary study of chemical synthesis, physiological effects, biological analysis of PSK. The main contents are as follows: 1. The sulfonation reaction Tyr at pentapeptide-Ile-Tyr-Thr-Gln sulfonation its sulfonation product was separated by column chromatography, then further purified by thin layer chromatography (TLC). , PSK-α synthesis successfully identified by mass spectrometry. 2. Lower initial density (3.6 × 10 4 a · ml -1 ) BY-2 suspension cell culture system, add the right amount of growth even in the medium the prime and cytokinin, cells can not be normal growth and division. The low density BY-2 suspension cells were added to the different concentrations of CM, the CM can promote the proliferation of BY-2 cells in the low density. The analysis showed: CM existence of mitogenic activity of signaling molecules. 3. We added to the chemical synthesis of different concentrations of PSK-α BY-2 cells in the low density culture system to verify that the physiological effect of the PSK-α. Measurement result display: in the low density culture systems, the chemical synthesis of PSK-alpha promote mitogenic activity and CM in the signal molecules of promoting wire splitting activity trends coincide, which shows the CM that promote cell proliferation signal molecule is PSK-alpha . in cell OD 600 within a certain range of concentrations with increasing concentration of PSK-α increased in 0.25nmol · L -1 ~ 5.0nmol · L -1 PSK-α concentrations within OD 600 and PSK-α concentrations a linear relationship. Therefore, the reaction system can be used as a PSK-α biological analysis system. 4. Identified by chromatography and mass spectrometry (MS) for the first time from the wheat callus, unfertilized wheat ovary young spike of wheat, corn pollen and tobacco BY-2 suspension cells CM found the presence of PSK-α. 5. Total RNA was extracted from wheat callus precursors of PSK genes by RT-PCR method. Sequence analysis showed that: we cloned DNA fragment deposited in GenBank clone wheat PSK precursor gene (Accession number BK000132) homology is 99.62%. This also shows that there wheat callus PSK precursor gene, wheat callus may be the presence of signal molecules PSK, it may be involved in wheat induced callus formation and plant regeneration.

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CLC: > Agricultural Sciences > Plant Protection > Pesticide ( chemical control ) > Various pesticides > Plant growth regulator
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