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With killing nematode - active plant endophytic bacteria and the rhizosphere actinomycetes screening and control efficiency

Author: PengShuang
Tutor: ZuoShuZhen
School: Nanjing Normal University
Course: Microbiology
Keywords: Plant parasitic nematodes Endophytic bacteria Rhizosphere actinomycetes Active product Control effect
CLC: S476
Type: Master's thesis
Year: 2011
Downloads: 32
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Abstract


Plant-parasitic nematodes are important pathogens harmful to plants with higher screening to plant parasitic nematodes kill nematode activity of the plant endophytic bacteria and plant rhizosphere actinomycetes. This study endophytic bacteria the nematicidal activity Isolation, screening and identification, and one of the most active strains the optimization of fermentation conditions; endophytic bacteria and rhizosphere actinomycetes fermentation products nematicidal substances stability; endophytic bacteria and actinomycetes prevention and control of root-knot nematode, and endogenous bacteria and actinomycetes Complex of root-knot nematode control effect; The results are as follows: 1, using tissue isolation from six 112 endophytic bacteria isolated and screened plants, no vaccine colonization amount assay screening 64 strains stable colonization with cucumber. To pine wood nematode target screening method with direct contact toxicity, this 64 stable colonization strains screened 13 pairs of pine wood nematode has a higher nematicidal activity of plant endophytic bacteria fermentation of these strains supernatant treated pine wood nematode ratios of 24h nematocidal reached 100%; including BCM2 four strains SZ5 CCM7 and DP1 high nematicidal activity, fermentation supernatant diluted three times the processing 24h nematicide rates were reached more than 95%, DP1 and SZ5 of strain reached 100%; and found that the part of the fermentation broth nematode worms can leak or digestion. While the article also 326 actinomycetes isolated soil nematicide activity screening nematicidal activity 21 actinomycetes, 21 actinomycetes fermentation supernatant loose 24h processing wood nematode mortality reached more than 80%, of which eight actinomycetes fermentation broth the nematicidal activity reached 100%, but some strains can produce substances make nematode digestion. After rescreening of 16 higher activity of actinomycetes, get five activity actinomycetes were: NK17, NK19, NK27, NK29 and V21 3, physiological and biochemical identification and 16SrDNA of sequence The determination method identified the 4 plant height active plant endophytic bacteria vesting types results identified DP1 and CCM7 of Bacillus subtilis (Bacillus subtilis), BCM2, and SZ5 is Bacillus cereus (Bacillus cereus). 4, the determination of stability after the study of the physical and chemical nature of the the nematicide metabolic substances and fermented liquid nematicide active substances, five actinomycetes produce nematicidal substances are a volatile small molecule alkaline substances; 4 endophytic bacteria in plants generated material is a protease stable, heat-resistant acid long preserved activity does not decrease small molecule peptides. 5, determined by single factor method four plant endophytic bacteria fermentation nematicidal substances best carbon and nitrogen sources and the 4 plant height activity of endophytic bacteria strains using orthogonal experimental method for the optimization of fermentation conditions, the results indicate that this 4 endophytic bacteria in plants produce the nematicide substances best carbon source is peptone, the best nitrogen source nematicide effect of fermentation broth beef extract, orthogonal optimized 4 times. 6, the activity of actinomycetes and plant endophytic bacteria on the root-knot nematode control effect and actinomycetes and endophytic bacteria composite control effect potted seedlings IRRIGATING Law. Actinomycetes tomato root-knot nematode control efficacy results better NK29 and NK17, 38.17% and 37.63%, respectively; tested seven plant endogenous endophytic bacteria the six plants DP1 outside bacteria on tomato root-knot nematodes have a certain amount of control effect, including better control effect of the BCM2 and DP24 on the tomato root-knot nematode, 57.35% and 50%, respectively, followed for CCM7 and DLJ1. The BCM2 and CCM7 treatment of experimental tomato root-knot nematode and prevention experiment, the results show that these two endophytic bacteria in tomato root-knot nematode disease treatment results are not ideal, but there is a certain effect in the prevention and control root-knot nematode . The actinomycetes bacteria and endogenous bacteria mixed control efficiency results show that: in the case does not affect plant growth on the root-knot nematode the inhibitory strains mixed BCM2 NK17, and CCM7 NK29 both complex plants were growing and abamectin treated fairly, and better than the water control treatment group, the root-knot nematode control efficiency of 57.81% and 37.89%, respectively. This study screened from bacteria to the product of the nature of the determination of identification and then control effect, a series of results proved soil and cash crops in vivo to produce a certain number of actinomycetes and plant nematicide active substances endophytic bacteria, which Some bacteria produce nematicidal substance has a strong stability. Think has great potential for biocontrol nematicidal activity of plant endophytic bacteria.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control
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