Dissertation > Excellent graduate degree dissertation topics show

Study on United Production of Vitamin B12 and Removal of Propionic Acid from Fermentation Broth

Author: ZhangJianFei
Tutor: WangYunShan
School: Hebei University
Course: Microbiology
Keywords: Vitamin B12 Fermentation coupled with separation Propionate Expanded bed
CLC: TQ920.1
Type: Master's thesis
Year: 2009
Downloads: 60
Quote: 0
Read: Download Dissertation

Abstract


Vitamin B12 is one of the important microbial fermentation products, widely used in medicine, food and livestock production by microbial fermentation. The papers from the application point of view, the initial substrate concentration, acid on cell growth and the synthesis of cobalamin, exploring the line separating propionic acid in fermentation engineering, the lifting of the organic acids (propionic acid) on the growth of bacterial cells suppression, expect to provide a reference for cobalamin fermentation production process improvements. This thesis is mainly the following aspects of the study. . Of substrate concentration on the influence of vitamin B12 fermentation investigated the concentration of the C source and N source concentration of Propionibacterium freudenreichii affect fermentative production of vitamin B12, the experimental results show that the glucose concentration was maintained at 2.0%, corn steep liquor concentration is maintained at 4.0% When most conducive to microbial growth. Taking into account the operability of the feeding during the fermentation process, the glucose concentration was maintained at 1.0% -3.0%, the concentration of the corn syrup is maintained at 3.0% -4.0% is more reasonable. Propionic acid fermentation of vitamin B12 in this experimental study plus propionate Propionibacterium freudenreichii growth effects of propionic acid suppression with replacement of fresh medium lift the Propionibacterium freudenreichii fermentation production of vitamin B12 the impact. The study showed that the concentration of propionic acid is applied at 0-0.5% bacterial μmax reduced to 60%, plus propionate 1.0% bacterial μmax to 30%. Replaced with fresh medium lift propionic acid suppression, biomass increased by 74.5%; deoxyadenosine cobalamin production increased by 40.7%. Reaction and separation coupled fermentation process initially explored using ion exchange resin adsorption lifting of organic acids inhibit the impact on the the Propionibacterium freudenreichii fermentation production of vitamin B12. Were studied using a fixed bed, expanded bed timing adsorption propionic and expanded bed adsorption propionic online fermentation process, can effectively relieve the organic acids on the inhibition of cell growth, to achieve the continuous growth of the cells, provides for the continuous fermentation production process reference. The new technology used in the production of bacteria, and achieved good results. 4 ceramic membrane preliminary study on the growth of bacterial cells ceramic membrane of the bacterial cell growth activity. The experiments show that the fermentation broth is concentrated through the ceramic membrane, the cell concentration to not before half of the growth activity of the cell damage caused. This experiment used in the process of fermentation and separation coupled to provide a theoretical basis for the ceramic membrane separation cell.

Related Dissertations

  1. Preparation and adsorption properties of activated carbon Camellia oleifera seed cake base,X792
  2. Study on Plasma Homocysteine and Asymmetric Dimethylarginine Levels in Patients with Acute Cerebral Infarction and the Effect of Intervention Treatment,R743.3
  3. The Research of Relationship between ADMA and Hcy about the Intervention Treatment in Essential Hypertention,R544.1
  4. Liquid Adsorption in Tubular Structured Ordered Mesoporous Carbon,O647.3
  5. The Study of the Relationship between Homocysteine and Acute Cerebral Infarction,R743.3
  6. Nutritional epidemiology survey of 2 to 7 -year-old children vitamin B_ (12),R181.3
  7. Yunnan People Folic Acid, Vitamin B12 Serum Levels, MTHFR Polymorphisms and Breast Cancer Susceptibility,R737.9
  8. Meta-Analysis on Relationship between Homocysteine, Vitamin B12, Folate and Multiple Sclerosis,R744.51
  9. Clinical studies of the relationship between homocysteine ??and cerebrovascular disease,R741.02
  10. The Studies and Applications of Fluorescence Resonance Energy Transfer System,O657.3
  11. Study on the Reaction Kinetics of Propylene Glycol Monomethyl Ether Propionate,O621.25
  12. Protective Effects of Folic Acid, Vitamin B6 and Vitamin B12 on Rats with Cerebral Ischemia,R743.3
  13. VB12 Nutritional Status in Children Aged 2～7 and Oral Therapies in Children with VB12 Deficiency,R723
  14. Effect of VitaminB1, VitaminB12 and VitaminH on the Growth and Astaxanthin Content of Haematococcus Pluvialis,Q943.1
  15. Clinical Study on the Increases of Plasma Homocysteine in Initial Days After Coronary Artery Bypass Grafting,R654.2
  16. β-cyclodextrin spectrometry,O657.3
  17. The Relationship between Recurrent Cerebral Infarction and Serum Folic Acid as Well as Serum Vitamin B12,R743.3
  18. Optimization of Vitamin B12 Fermentation Process by Integration of Microbial Macro and Micro Metabolic Analysis in the Context of Industrial Systems Bioprocess Engineering in Pseudomonas Denitrificans,TQ924
  19. The Effects of Arsenite on the Apoptosis of Human Umbilical Vein Endothelial Cells (HUVECs) and the Underlying Mechanism,R599
  20. The Research on Therapeutic Effect of Testosterone Propionate on Mice Autoimmune Premature Ovarian Failure and Its Mechanism,R711.75
  21. Effect of Propionic/Acetic Acid and pH on Enriched Culture of Glycogen Accumulation Organisms (GAOs),X703

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Fermentation industry > General issues > Basic theory
© 2012 www.DissertationTopic.Net  Mobile