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Buckwheat is a diploid annual crops, widely grown in Asia, Europe. Buckwheat can be grown at high altitudes, short growing season, seed protein content, in our part of the mountain and the northern hemisphere are important food crops in some countries. Buckwheat balanced amino acid content, contains rutin, quercetin, kaempferol ol-3 - rutinoside medicinal ingredients such as flavonoids. Buckwheat mostly planted in the barren land, production is not high, by molecular marker-assisted breeding methods to improve their resistance, improved quality and yield buckwheat industrial development is an important issue. Genetic mapping is the basis for marker-assisted breeding, but also the plant genome research is one of the important contents. Has an important economic value for buckwheat, building a genetic map can be used to good genes and important agronomic traits mapping, marker-assisted breeding of great significance. In this study, Yunnan Ning One (female) and wild buckwheat (male) on behalf of the 136 F2 mapping population, using two molecular markers, applicable to buckwheat SRAP and SSR reaction system and the separation of labeled analysis and other aspects of the research, and build a current first buckwheat molecular genetic linkage map. Specific studies are as follows: the use of interactive orthogonal design L27 (313) on buckwheat SRAP-PCR, SSR-PCR reaction system for the five factors (Mg2, dNTP, Taq enzyme, the template DNA and primer) three levels of optimization experiments and conduct validation, the best SRAP-PCR reaction system: the total volume 20μl, Mg2 1.5 mmol / L, dNTP 0.2 mmol / L, Taq enzyme 1.5U, template DNA 40 ng, primers 0.25μmol / L, 10 × buffer2μl; Best SSR-PCR reaction system: the total volume of 20μl, Mg2 1 mmol / L, dNTP 0.3 mmol / L, Taq enzyme 1.5 U, template DNA 30 ng, primers 0.25μmol / L, 10 × buffer 2μl. The PCR products were non-denaturing gel electrophoresis with denaturing PAGE and two kinds of operating systems, and the results show that the non-denaturing PAGE, DYCZ-24F operating system is more suitable for SRAP analysis. The use of 165 pairs of SRAP primers parents and F1 hybrid DNA, polymorphic primers screened 43 pairs of polymorphic ratio of 26.1%. With 43 pairs of primers F2 population polymorphism analysis, 50 polymorphic markers were obtained, including primers M1E2, M3E3, M6E19, M10E4, M11E17 produce two polymorphic markers, respectively; primer M1E6 produce three polymorphisms tags. The chi-square test, obtained 50 polymorphic markers, there are 35 (70%) SRAP markers Mendelian ratio. From 148 pairs of SSR primers were screened to 26 pairs of polymorphic primers, polymorphism ratio was 17.6%. With 26 pairs of polymorphic primers F2 populations were analyzed, only one pair of primers labeled without segregation phenomenon, all the other 25 pairs of female biased. Using the Mapmaker / EXP 3.0 software build 10 linkage groups containing from 37 SRAP markers, SSR markers consisting of a genetic linkage map. The map length of 725.1 cM, marking the largest map distance 46.6 cM, minimum map distance 2.2 cM, an average distance of 25.9 cM; linkage group marker numbers between 2-6, LG6, LG6 contains markup Up , both six; LG3, LG8, LG9 contain a minimum number of marks for two. In this thesis, buckwheat genetic linkage map constructed, the advantage is marked on the map with a clear, specific amplification, good repeatability; drawback is less marked, low density and requires further encrypted by SRAP markers to establish a more complete molecular genetic buckwheat linkage map.
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