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Xinjiang Ligusticum genus (Coniselium vaginatium Thell) the Umbelliferae (Umbelliferae) Mountain Xiong (Coniselium), Chinese herbal medicine for the common solution table class. Expelling wind, cold, dampness, analgesic effect. Clinical Indications cold cold, top of the top headache, rheumatism limb Bitong topical treatment for scabies ringworm tumor. Xinjiang Ligusticum resources yield higher levels of volatile oil, has a very good use of value. The main active ingredient in Xinjiang Ligusticum volatile oil, which contains phellandrene chemical composition of ligustilide myristicin elemi prime. At home and abroad Xinjiang ligustilide, do a chemical analysis, separation, synthesis and pharmacological research, Xinjiang Ligusticum different extraction methods, mathematical model of extraction process and the extraction process at home and abroad rarely reported. Current Xinjiang ligustilide also just used in the pharmaceutical industry, but its processing is relatively primitive, yet to be widening range of applications, flavors and fragrances, nutraceuticals and cosmetics industry has not been reported, the preliminary experiments to explore the application to tobacco flavoring. The purpose of this study is to better use the Xinjiang ligustilide extracts, botanicals resources to accelerate the effective development and utilization. This paper focuses on the following aspects: 1. Compared different extraction methods impact on the Xinjiang ligustilide volatile oil. By steam distillation, microwave, ultrasound, reflux, Soxhlet extraction method to extract the volatile oils in Xinjiang Wara, simultaneous determination of the refractive index and density of Xinjiang Wara several different extraction methods resulting physical properties of the volatile oil, and applying mass spectrometry technique its volatile oil content and composition analysis. Chromatographic conditions: GC conditions: HP-5 MS fused silica capillary column (30 m × 0.25mm x 0.25um), carrier gas He, flow rate 0.8ml/min, inlet temperature of 250 ° C, the injection volume 1ul split ratio 50:1 temperature program 60 ° C to maintain 1min, 4 ℃ / min raised to 230 ℃, for 10 minutes. MS conditions: the ion sources EI, electron energy 70Ev, ion source temperature of 250 ° C, transfer line temperature of 280 ° C, and the quality of the scan range 35-500 amu. Results volatile oil yield: 2.40%, 2.19%, 6.43%, 6.75% and 9.73%, respectively. 44,41,40,40,37 chemical composition were identified. The Soxhlet mention obtain the highest oil yield, but also more impurities, contains a lot of fatty oil. Microwave, ultrasound has the characteristics of high efficiency. The volatile oils of various extraction methods to get a little different, volatile oil steam extraction is superior to several other. Xinjiang Ligusticum volatile oil extracted by steam distillation, the use of a single factor and orthogonal experimental design preferred optimum extraction conditions influence the amount of water, soak time, extraction time, particle size of the four factors of the volatile oil extracted. The optimum extraction conditions were: 40 mesh sieve, 12 times the amount of water, soak for 6 hours to extract five hours. Optimum process to extract in Xinjiang ligustilide, volatile oil yield of 2.4%. Create two mathematical models of the Xinjiang ligustilide volatile oil extraction process experimental data fitting. Xinjiang Ligusticum volatile oil extracted dynamic model equation: V = 2.04 * (1-exp (-0.01018t)) (R-2 = .99817) and ln (2.1-V) = 0.00944t .72895 (R-2 = 0.9979 ). 4 will be the steam distillation proceeds Xinjiang ligustilide volatile oil and other Chinese herbs extracts carried out the tobacco flavoring experimental contrast. The smoking results show that the the Ligusticum volatile oil of cigarettes has significantly the flavoring to mention the quality of role. The innovation of this study is to: 1. Optimized Xinjiang ligustilide the volatile oil extraction process, reported volatile oil production rate increased to 2.40% from 1.03%. It is preferable that the extraction process is feasible, and the results are stable. Established kinetic model equations Xinjiang ligustilide the volatile oil extraction process, its simulation and the experimental values obtained in good agreement with the mathematical model regression effect, applicable to Xinjiang the Wara this volatile oil extraction process optimization control. Compared different extraction methods to extract the Xinjiang ligustilide volatile oil, volatile oil content was measured physical parameters as well as by GC-MS analysis of the chemical composition, and a total of 59 compounds analyzed and detected. And to explore the advantages and disadvantages of Xinjiang Ligusticum 5 kinds of extraction methods. 4. Taking into account the higher the Xinjiang ligustilide the volatile oil content and flavor strong initial exploration research Xinjiang ligustilide the volatile oil in tobacco flavored.
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