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Study on the Enzymatic Saccharification and Thermogravimetry of Corn Stover Pretreated by Mixed-culture of White-rot Fungi
Author: WangJinJin
Tutor: YangYang
School: Huazhong University of Science and Technology
Course: Microbiology
Keywords: White rot fungi Mixed culture Cellulase Lignocellulose TGA
CLC: S216.2
Type: Master's thesis
Year: 2009
Downloads: 51
Quote: 0
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Abstract
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Corn stover for ethanol production has major economic significance . However , due to its complex structure , the need using pretreatment to improve the rate of enzymatic saccharification . This paper degradation characteristic differences between the strains , mixed culture of white rot fungi mainly processing corn stalks , mixed culture of enzymatic saccharification a preliminary understanding of the mixed culture of thermal heavy impact . Screened 22 157 Basidiomycetes , and then deal with different combinations of corn stalks , enzymatic saccharification rate compared with pure cultures of mixed cultures treated did not significantly improve . To further study the mixed culture of enzymatic saccharification select different degradation patterns of white rot fungi , Auricularia / ZT, Auricularia / CD2 mixed culture system by adjusting the proportion of inoculation and vaccination order , and to study the mixed culture of white rot bacteria enzyme characteristics lignocellulose degradation characteristics . The main conclusions are: enzyme activity compared with pure cultures of the occurrence of a significant change , showed synergistic or suppress ; mixed culture can improve the rate of degradation of cellulose , lignin degradation rate is somewhere between pure culture , mixed culture of different strains of a combination of half- fiber prime degradation rate trends ; enzyme activity and lignocellulose degradation rate of no correlation . Decline in cellulose content , lignin retention may result in mixed culture saccharification rate is lower than the pure culture . TGA experiment result table understand rot fungus mixed culture can lead to reduce the initial temperature of pyrolysis , the termination of the temperature rise , and the solid residue was increased , showing the pure culture of the same law . From the activation energy for the mixed culture than in pure culture is not conducive to the thermal cracking . Mixed culture , which led to the decline of the relative content of cellulose , lignin relative content related to an increase . Through this study provide a theoretical basis for applications in biological pretreatment of biomass production of fuel ethanol and bio- oil mixed culture .
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CLC: > Agricultural Sciences > Agricultural Engineering > Agricultural power,rural energy > Application of bio-energy ( biomass) > Plant energy
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