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Inhibition Effect of Curcuma Oil Injection on Porcine Parvovirus in Vitro

Author: XuDuanHong
Tutor: KuangLing
School: Xinjiang Agricultural University
Course: Clinical Veterinary Medicine
Keywords: Curcuma oil injection PPV SFE Real-time PCR MTT
CLC: S853.7
Type: Master's thesis
Year: 2010
Downloads: 42
Quote: 0
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Abstract


The purpose of this study is to explore the field of traditional Chinese medicine disease inhibition of the Curcuma oil extraction method and its injection of porcine parvovirus ( PPV ) , to provide the experimental basis for the prevention and treatment of PPV disease . Test the use of supercritical carbon dioxide extraction ( SFE ) Curcuma oil , optimum conditions : 60 mesh , temperature 55 ° C , pressure of 25MPa . Gas mass spectrometry ( GC - MS ) Curcuma oil, containing Curcuma alcohol , curzerenone , Lam Hong ene and other ingredients . Proceeds turmeric oil, prepared a final concentration of 1% Curcuma oil injection . The maximum non-toxic concentration ( TC0 ) 1% the Curcuma oil injection role in PK-15 cells , cytopathic effect ( CPE ) method 62.5μg/mL , half of infected concentration ( TC50 ) 125μg/mL , ; dye method ( MTT ) measured TC0 28.67μg/mL TC50 49.33μg/mL . Designed according to the results of the MTT assay toxicity of three different dosing methods , in order to explore the mechanism of the Curcuma oil on the role of the PPV . The results show that inhibit viral effect of the mode of action of the virus before adding the drug plus Preferably, the in vitro EC50 IC50 2.813μg/mL, 90% effective inhibition concentration IC90 4.123μg/mL , the therapeutic index is 31 ; virus control was statistically significant ( P lt ; 0.01 ) , with the positive control drug ribavirin was not statistically significant ( P gt ; 0.05 ) Select before adding drug and virus mode of action, to explore the Curcuma oil injection in the effective concentration range inhibition of the PPV proliferation law . One-way ANOVA , compared with the virus control , role - within 12 hours of the virus DNA copy number change was not statistically significant ( P gt ; 0.05 ) , after 12 hours , the amount of PPV DNA copy was significantly lower than the virus control group , statistically significant ( P lt ; 0.05 ) , 65h PPV DNA copy , the difference was significant ( P lt ; 0.01 ) Supercritical carbon dioxide extraction ( SFE ) Curcuma can get high quality Curcuma oil ; the Curcuma oil injection liquid anti- virus test the best role in the effect of the first drugs plus virus ; the Curcuma oil injection liquid porcine parvovirus has inhibitory vaccine failures or wild virus infection to available 4.123-28.67μg/mL Curcuma oil injection PPV disease prevention .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Chinese veterinary science > Veterinary Pharmacology
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