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Research on KAP13.1 and IGF-1 Gene Genetic Variation and Association with Economic Traits of Goat Breeds in Xinjiang

Author: FangYi
Tutor: LiuWuJun；ChenHong
School: Xinjiang Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Cashmere goats Economic traits Candidate genes Genetic variation PCR-SSCP PCR-RFLP
CLC: S827
Type: Master's thesis
Year: 2010
Downloads: 76
Quote: 1
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Abstract


The test to a total of 776 individuals from three goat breeds of Xinjiang goat, the Nanjiang cashmere goats and the Bogda cashmere goats for material, the use of PCR-RFLP and PCR-SSCP, bioinformatics and sequencing technology, to analyze KAP13.1 gene and IGF- molecular characteristics of a gene and its relationship with of goat cashmere production performance. At the same time, Xinjiang goat population genetic structure, genetic polymorphisms and economic traits, to obtain the corresponding molecular genetics information, to find DNA markers and economic traits for the the cashmere goat genetic resources conservation, development and use, as well as DNA markers in marker-assisted selection to develop and goat production performance improvement and the improvement of scientific basis. In this study, the following important findings: 1 KAP13.1 gene genetic variation and its relationship with economic traits of this test to study an HS-KAP KAP gene family gene KAP13.1. Three cashmere goat groups in Xinjiang goat, the Nanjiang cashmere goats and Bogda cashmere goats of, KAP13.1 gene multiple sites mutated KAP13.1 genes were revealed five SNPs, respectively for c.67G gt; A gt;, c.115T gt; C, c.124C T c.186T gt; G and c.213C gt; G. c.67G gt; A c.115T gt; C, c.124 C gt; T are synonymous mutation c.186 T gt; G and c.213 C gt; G occurring missense mutations cause transversion bases, which c.186 T gt; G missense mutations lead to amino acid p.48 In 1990, Leu gt; Arg change c.213 C gt; to G missense lead to amino acid p.57 Thr gt; Ser change Further analysis revealed that the the c.186T gt; G mutation leads to reduced KAP13.1 gene Pvu Ⅱ enzyme locus, resulting Pvu Ⅱ enzyme polymorphism loci. The results showed that the polymorphism KAP13.1 genes exist in Xinjiang goat, Nanjiang cashmere goat, and Bogda cashmere goat populations, and in Hardy-Weinberg non-equilibrium state (P gt; 0.05) in Xinjiang goat populations was not detected in the heterozygous genotype, and mutant homozygous individuals only detected one described KAP13.1 gene polymorphism may not Xinjiang goat varietal characteristics, may be related to the genetic stability of Xinjiang goats. The chi-square test for independence of genotype and allele in this locus, the differences between the three goat populations extremely significant (P lt; 0.01), according to the results of previous studies, it was found KAP gene in different significant variation between varieties, it is the accumulation of these mutations caused by the diversity of different physical and chemical properties of the cashmere goat cashmere. The polymorphic loci with three cashmere goat populations traits and fleece weight after variance analysis showed that, in southern Xinjiang cashmere goat the the KAP13.1 gene detected by the test thickness traits and fleece weight after traits have a significant impact ( P lt; 0.05). Genetic variation 2.IGF-1 gene and its relationship with economic traits in this experiment to study the IGF-1 gene P1, P2, P3, P4 four loci. Xinjiang goat, the Nanjiang cashmere goat and Bogda cashmere goats three goat breeds groups IGF-1 gene loci found that there are two SNPs in the IGF-1 gene P1 locus c.1617 G gt; A and c.1620 C gt; T due to base conversion, further analysis found that are synonymous mutations. Polymorphic information content of 0.30 to 0.50 between two SNP groups, belonging to the moderate polymorphism. The genotype distribution of the chi-square test of independence showed a significant difference between the three goat populations (P lt; 0.01). IGF1-P1 polymorphic loci Xinjiang goat cashmere production traits had no significant effect (P gt; 0.05). In in Nanjiang cashmere goat cashmere production traits and fleece weight after analysis of IGF1-P1 polymorphic loci AA genotype cashmere fineness significant fineness below AB genotype (P lt; 0.05) The AC genotype fleece after weight significantly higher than BB genotype (P lt; 0.05).

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
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