|
Of this research project is to take advantage of the the Shihezi City West Park feeding Xinjiang wild argali unique conditions, electrical stimulation of semen on the argali, dead azoospermia treatment, preliminary study of semen cryopreservation, estrus synchronization and superovulation technology and bred hybrid generation, second-generation sheep blood biochemical indices were measured and analyzed, and achieved certain results. Argali electrical stimulation of the semen and dead azoospermia treatment electricity stimulation of argali artificial semen collection technology, has 59 times semen, semen successful 53 times, the success rate of 89.8%. The argali different output voltage electrical stimulation can cause varying degrees of erectile response, with the gradual increase in the number of semen, 8V output voltage of about stimulus, more prone to penile erection and ejaculation reaction. 7-year-old age argali after treatment, sperm count increased significantly, activity was significantly enhanced, so that the ewes to conceive a child. 3-year-old age argali after treatment, sperm count and vitality no significant change. Successfully collected the the argali semen, sperm motility and density were better, the electrical stimulation did not affect the semen quality of the argali, a suitable argali semen. 2 the argali the semen freezing technology research in order to evaluate accurately the effect of argali semen frozen, we design a three dilution formula of argali and domestic of four corners sheep's semen semen dilution under the same conditions, freezing, preservation and thawing contrast. The 3 # diluent Tris3.63g, glucose 0.50 g fructose, 1.99g, 0.63g of sodium citrate, taurine 0.63g, penicillin 100,000 IU, 100,000 IU of streptomycin yolk 15ml, glycerol 12% of the frozen semen After thawing, the argali, the four corners sheep sperm motility, respectively, 0.23 ± 0.06 and 0.31 ± 0.15, better. Thawing of the argali frozen semen activity was significantly lower than the four corners of the sheep, in addition to fresh semen vitality of the four corners of sheep than argali, whether varieties, physical structure, and environmental factors such as, pending further study. Estrus synchronization technology research use of progesterone sustained release vaginal suppository (CIDR) the prostate hormone (PG), pregnant mare serum gonadotropin (PMSG), promote ovulation No. 3 (LHRH-A3) argali, Bashibai sheep and their F1 sheep and other varieties of sheep were estrus synchronization. Estrus rate argali 75% the F1 generation sheep, 68%, and 80% Bashibai sheep, other varieties of sheep was 93.33%. The the estrus rate argali, F] generation sheep and other varieties of sheep between different significantly (P lt; 0.01); Bashibai sheep with the F1 generation sheep, and other varieties of sheep between significantly different (P lt; 0.05). Argali F1 generation sheep estrus rate is basically the same, but significantly lower than the other varieties of sheep, to the argali genetic stability, hybrids The sheep may already have the physiological characteristics of the argali. Superovulation technology research use of progesterone sustained release vaginal suppository (CIDR), prostate the prime (PG), pregnant mare serum gonadotropin (PMSG), follicle-stimulating hormone (FSH), ovulation 3 (LHRH-A3) argali the F1 generation sheep, domesticated sheep superovulation. Difference between the ovulation number of argali the F1 generation sheep and domestic sheep was significantly (P lt; 0.01); the average ovulation number of argali F1 generation sheep and domestic sheep difference between a very significant (P lt; 0.01), F1 generation sheep and the significant difference between domestic sheep (P lt; 0.05); the ultra-lined power and average number of ovulation argali 75% (3/4) and 3.3 / head F1 generation sheep was 100% (3/3) and 16 / head, domestic sheep to 100% (3/3) and 20.7 / head superovulation better response overall. Blood physiological indicators of the the erythrocyte total number of the measurement and analysis of argali (RBC), hemoglobin (Hb), packed cell volume (PCV) was significantly higher than Bashibai sheep, the F1 generation sheep and the F2 sheep, the difference was significant (P lt; 0.01). Platelet count (PLT), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC) argali Bashibai sheep and F1 generation sheep significantly lower than the F2 sheep, a significant difference (P lt; 0.01). Total number of white blood cells (WBC) argali and Bash worship sheep between the difference was not significant (P gt; 0.05), but significantly higher than the F1 sheep F2 generation sheep, a significant difference (P lt; 0.01); Bashibai sheep significant differences between the F1 generation sheep (P lt; 0.05); F1 generation sheep F2 generation sheep between the difference was not significant (P gt; 0.05). Lymphocytes (LYM) argali significantly higher than Bashibai sheep, the F1 generation sheep F2 generation sheep, a significant difference (P lt; 0.01), while Bashibai sheep F1 generation sheep, no difference between the F2 generation sheep significantly (P gt; 0.05). Intermediate cells (MID) and granulocyte (GRAN) argali significantly lower than Bashibai sheep, the F1 generation sheep and F2 sheep, the difference was significant (P lt; 0.01) 6. Determination of blood biochemical indicators were analyzed for glucose (GLU ) argali of higher than Bashibai sheep, a significant difference (P lt; 0.05); Bashibai sheep was significantly lower than the F2 sheep, and a significant difference (P lt; 0.01). Total bilirubin (TB), direct bilirubin (DB) the argali and F2 generation sheep significantly lower than Bashibai sheep and the F1 generation sheep, the difference was very significant (P lt; 0.01). Alanine aminotransferase (ALT) argali higher than Bashibai sheep, a significant difference (P lt; 0.01); below the F2 Bashibai sheep sheep, a significant difference (P lt; 0.05); the F1 generation sheep, is lower than argali, a significant difference (P lt; 0.05). Content of various mineral elements, in addition to the sodium content of elements than normal high reference value, the remaining elements of the content in between the argali, sheep Bashibai, F1 generation sheep and the F2 sheep there are some differences, but all within the in sheep normal reference range, and the variation in the six elements of the F1 generation sheep and F2 generation sheep argali characteristics This shows strong argali hybrids sheep adaptability to the environment, the offspring of sheep genetic stability .
|