Dissertation > Excellent graduate degree dissertation topics show

The Analyse of the Relation of Polymorphism of MHC-DRB1 Gene and Hydatidosis in Xinjiang Hazakh and Chinese Merino Sheep

Author: WenQingNa
Tutor: JiaBin
School: Shihezi University
Course: Animal Genetic Breeding and Reproduction
Keywords: Sheep Hydatid disease MHC-DRB1 PCR-SSCP
CLC: S826
Type: Master's thesis
Year: 2010
Downloads: 23
Quote: 0
Read: Download Dissertation

Abstract

Echinococcus granulosus disease (Cystic Echinococcosis, CE), also known as hydatid disease by Echinococcus (Echinococcus granulosus, Eg) larvae (metacestode) parasitic in a variety of intermediate hosts (and herbivores such as a zoonotic disease of cattle, sheep), liver, lungs, and other organs developed cysts cause. Xinjiang is China's animal husbandry production base, hydatid disease has become the most serious harm to human health in Xinjiang endemic, causing great losses to Xinjiang livestock production. In recent years, the world of echinococcosis has gone deep into the cellular and molecular levels, and great achievements, the incidence rate has decreased significantly, but fundamentally it is very difficult to control and eradicate the disease. The major histocompatibility complex (major histocompatibility complex, MHC) is tightly linked to the composite body of highly polymorphic loci, widely present in vertebrates. MHC molecules combined with foreign antigens and these antigens are presented to T lymphocytes, and eventually cause the body's immune response, and therefore, of MHC with many disease resistance, susceptibility, immune response and the production performance are closely related. MHC as disease resistance and susceptibility candidate genes, Molecular Immunology today genetic research has become one of the hot spots. This study Kazak sheep and Chinese Merino Sheep (Xinjiang Merino type) MHC-DRB1 gene polymorphism study and explore the MHC-DRB1 gene and echinococcosis using marker-assisted selection breeding sheep new disease-resistant varieties, and lay a foundation for early disease resistance mechanism to further study the MHC, the test results are as follows: 1. 200 Kazak sheep and 205 Merino sheep using nested PCR-SSCP (which have their own 100 and 105 sheep infected with hydatid disease) MHC-DRB1 exon 2 polymorphism study, the experimental results show that, Kazak and Chinese Merino sheep MHC-DRB1 gene exon 2 there are a wealth of polymorphism and two varieties of sheep were detected 15 alleles, genotypes, respectively, 37 and 41. Kazak sheep and Chinese Merino sheep hydatid disease negative antibody positive sheep MHC-DRB1 allele and genotype frequencies were compared, results showed that the Kazak sheep allele B, H and F (P lt; 0.01) Echinococcus granulosus disease resistance; the genotype FF and GH (P lt; 0.05) Echinococcus hydatid disease resistance-associated; allele K and G (P lt; 0.01) Echinococcus granulosus disease susceptibility; genotype GG (P lt; 0.01) and KK (P lt; 0.05) with Echinococcus hydatid disease susceptibility. Chinese Merino sheep population allele D (P lt; 0.05) with Echinococcus hydatid disease resistance; the related genotypes LG (P lt; 0.05) Echinococcus hydatid disease resistance; allele K (P lt; 0.05) with Echinococcus granulosus disease susceptibility; genotype GF (P lt; 0.05) Echinococcus granulosus disease susceptibility. 3.χ2 fit test results show that, Kazak and Chinese Merino sheep detect groups of MHC-DRB1 gene exon 2 SSCP patterns were not up to the Hardy-Weinberg equilibrium (P lt; 0.01). Two sheep breeds section genotype cloned and sequenced, and the results show that the MHC-DRB1 gene exon 2 the existence of a number of sites mutation, largely led to amino acid variation.

Related Dissertations

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Sheep
© 2012 www.DissertationTopic.Net Mobile