Dissertation > Excellent graduate degree dissertation topics show

Endophytic Fungal Diversity and Their Secodary Metabolites of Eleven Dominant Plant Species in the Dry-hot Valley of Yuanjiang, Yunnan Province, China

Author: HeCaiMei
Tutor: LiHaiYan
School: Kunming University of Science and Technology
Course: Biochemical Engineering
Keywords: dry-hot valley endophytic fungal diversity Phomopsis sp. antifungal activity secondary metabolites
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
Read: Download Dissertation


Seven hundred and eight endophytic fungi were isolated from 1147 stems and leaves segments of 11 dominant plant species Atylosia scarabaeoides, Azadirachta indica, Phyllanthus emblica, Broussonetia papyrifera, Sida acuta, Lannea coromandelica, Alysicarpus vaginalis, Calotropis gigantea, Acacia farnesiana, Flemingia philippinensis and Ocimum basilicum, which grow in the dry-hot valley of Yuanjiang county, Yunnan Province, southwest China. The isolation rates of endophytic fungi were between 0.44-0.92. It was found that the isolation rates of endophytic fungi from leaves were significantly higher than that of stems (P=0.043). Based on their morphological characteristics, the isolated fungi were finally identified to 29 taxa including Phomopsis sp., Bipolaris sp. and Alternaria sp. etc, which included 156 mycelia steriles,512 mitosporic fungi and 40 Ascomycetes.Phomopsis, Bipolaris, Peyronellaea, Ascomycetes, Aspergillus and Alternaria were also the dominant fungi of plant species in the dry-hot valley and the percentage of them were 34.18%,8.33%,7.34%,7.06%,3.53%and 2.26%, respectively. The endophytic fungi richness of the 11 plant species was different. The highest endophytic fungi richness appeared in,A. scarabaeoides, P. emblica, S. acuta and O. basilicum, in which 11 taxa were found, whereas, the lowest endophytic fungi richness appeared in L. coromandelica, in which only 5 taxa were found. The results showed that Phomopsis sp.(242,34.18% in all) was the most widely fungi of the dominant plant species in the dry-hot valley, its relative frequency got a range of 12.90% to 68.18%. The diversity of the community composition of endophytic fungi analysis suggested that the endophytic fungal diversity of dry-hot valley plants of Yuanjiang, Yunnan was lower than that of other environmental plant species.243 isolates were screened for their anti Candida albicans activity, and the results showed 29 isolates,11.93% of test strains.29 antifungal trains for secondary sereening, and the results showed that there were 16 trains secodary metabolites of antifungal activities, which the Phomopsis sp. was 13 strains,81.25% of antifungal trains. The higher 0antifungal activities isolates appeared in L.coromandelica and A.farnesiana, the inhibitory circle diameter was between 15-20mm.Compound LX-1 and LX-2 were isolated from the strain J642, which has been identified to be Phomopsis sp. and showed the highest anti Candida albicans activity. Compound LX-2 was identified asβ-D-Glucopyranoside based on its MS and NMR daters but compound LX-1 was still was not identified and still need further work. LX-1 showed anti Candida albicans activity, the inhibitory circle diameter was 16mm.

Related Dissertations

  1. Chinese television media discourse paradigm of the evolution of the multi-angle analysis,G220
  2. Survey and Analysis of Mycorrhizal Diversity in Zijin Mountain,S718.81
  3. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  4. Growth Pattern of Marsupenaeus Japonicus and Succesion of Plankton Community in Intensive Model,S968.22
  5. Study on the Secondary Metabolites of Two Fungi from the South China Sea,R284
  6. Shiraia its separation of fungal genetic diversity analysis,S567.39
  7. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  8. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii (Dunker) Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  9. Research on Optimization of the Relay Node Location of Cooperative Diversity,TN925
  10. The Study of Winter Waterbirds’ Diversity and Habitat Selection in the West Xinghua Bay Fujian Province,Q958
  11. Preliminary Study on Bacterial Diversity in Yunnan Non-ferrous Metals Mining,TD926.4
  12. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  13. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  14. Study on the Secondary Metabolities from Two Fungi,S482
  15. Genetic Diversity and Ultrastructural Studies of Southern Corn Rust in China,S435.131.4
  16. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  17. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  18. Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut,S565.2
  19. Effect of Organic Nutritiveadditives on Soil Micro-ecological Restoration and Its Mechanism.,S143
  20. Molecular Characterisation Based on ITS and Genetic Diversity of the Heterodera Avenae Group on the Huang-huai Floodplain of China,S435.121

CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental Microbiology
© 2012 www.DissertationTopic.Net  Mobile