Dissertation > Excellent graduate degree dissertation topics show

Studies on Formation and Enlargement of Lily (Lilium L.) Bulblets in Vitro

Author: RenYaPing
Tutor: ChenLongQing
School: Huazhong Agricultural University
Course: Ornamental Plants and Horticulture
Keywords: Lily Bulblet Tissue culture conditions
CLC: S682.29
Type: Master's thesis
Year: 2008
Downloads: 75
Quote: 0
Read: Download Dissertation

Abstract


Asian lily hybrid system (Lilium.asiatic hybrids) to longiflorum (Lilium.longiflorum), two species 'Acuba', 'Nove Cento' lancifolium (Lilium.lancifolium) no vaccine for explants from culture conditions start, to explore optimization promoting Bulblet swollen medium, provide a theoretical basis and key technologies for the the Lily Bulblet factory production. The conclusions are as follows: 1. Longiflorum filaments, pedicels explants induced to form plantlets were. The study found that the of medium MS 6-BA 0.5 mg / L NAA 0.1 mg / L suitable filaments callus induction and differentiation, suitable pedicel induced callus differentiation medium MS 6-BA1.0mg / L NAA0.5mg / L . MS 6-BA1.0mg / L NAA0.1mg / L most suitable aseptic subculture, the highest propagation coefficient, 5.29. 2 different culture conditions Bulblet enlargement. Within a certain range, increasing the sugar concentration to promote enlargement of the bulb, but the concentration is too high (120g / L) will affect the formation of the bulb, 90g / L For longiflorum and Asia lily 'Acuba' are most suitable for the concentration of the bulb of cultured average fresh weight of bulbs were 107.86mg and 127.97mg. The auxin NAA bulbs enlargement facilitating role. In medium supplemented with 0.5mg / L NAA, the most suitable the longiflorum Bulblet enlargement, its average bulb fresh weight 122.64mg: 'Nove Cento', 2.0mg / L NAA suitable bulb enlargement culture, access to the bulb Average fresh weight 124.22mg. 5mg / L in the medium supplemented with PP 333 can make the longiflorum get the most bulbs fresh weight, 99.22mg; 10mg / L PP 333 fit 'Nove Cento' bulb enlargement culture, to obtain the average the scales weight for 105.06mg. Orthogonal experiment, 'Acuba' choice of 90g / L sucrose, 10 mg / L PP 333 and 0.025mmol / L SA the bulb oriented culture, to get bulbs average fresh weight 161.17mg; Nove Cento 'choice of 90g / L sucrose, 1mg / L PP 333 the bulb of training, and 0.1mmol / L SA get bulbs average fresh weight 209.93mg. Activated carbon with a strong promotion of growth and rooting effect, also has strong promote bulb formation effect longiflorum and lancifolium of can use 3g / L AC bulb of culture, the bulbs average fresh weight as control 2 to 3 times. The effect of methyl jasmonate (Me-JA) weight gain musk lily bulbs, bulbs average fresh weight reached 110.77mg; methyl jasmonate For lancifolium, and no significant effect. Liquid shallow standing due bulbs easier absorption of nutrients, and therefore promote bulb enlargement, musk lilies and lancifolium of the largest bulb fresh weight in liquid culture were 161.62mg and 249.84mg than 8mm The large bulbs reached 48.53% and 54.05%, respectively, such training methods can also shorten the bulb incubation time to 60 days. Liquid medium, add sucrose and activated charcoal can to promote Bulblet enlargement, this medium will strongly promote the leaves and root growth, as well as in vitro bulb enlargement, including any kind of effect used alone much better. 60g / L sucrose 3g / L activated carbon most beneficial longiflorum Bulblet enlargement average fresh weight 627.32mg, the 13mm bulbs up to 39.02%; 90g / L sucrose 3g / L activated carbon the promote lancifolium Bulblet swollen optimal concentration, the average fresh weight 479.17mg, 13m above the bulb obtained up to 10.25%. 3 the solid medium Bulblet press diameter divided into A: 8mm, B :5-8mm and C :3-5mm three levels after sowing cultivation longiflorum survival rates were 95.8%, 81.9% , 80.6%. The Asian Lily 'Nove Cento' survival rates were 85.7%, 68.3%, 44.4%. Sprout growing enhanced with the increase of the diameter. The D: to obtain volume Dan Bulblet of liquid medium was divided according to diameter size above 12mm, E :9-12mm and F :7-9mm three levels, after sowing cultivation after lancifolium survival rate above 85%. 12mm more than the most robust bulbs germinate plants, 60 days after the bulbs still more than 7mm in diameter.

Related Dissertations

  1. A Model for Predicting the Flowering Date and External Quality of Lily Grown in Traditional Chinese Solar Greenhouse,S682.29
  2. AttM Transgenic Colored Calla Lily and Antibodies Preparation of Ahl-Degrading Enzyme AttM,S682.264
  3. Current Production Situation of Longya Lilium in Shaoyang and Studies on Its in Vitro Buld Induction as Well as Detoxification,S682.29
  4. Study on Introduction and Regional Trial of Oriental Lily,S682.29
  5. Extraction, Purity and Structure Identification of Lily Polysaccharides,TQ461
  6. Studies on Quality Control and Effective Substance Basis of Baihe Zhimu Tang,R286
  7. Study on Cut Lily Diseases and Bulbs Coating Chemicals in Gansu Province,S436.8
  8. Research of Overcoming Cross-incompatibility between Distantly Related Species of Lilium,S682.29
  9. The Effect of SA on Lily Bulb Development,S682.29
  10. Studies on Investigation、Collection and Protection of Wild Lilium Germplasm Resources from Qinba Moutain Aera,S682.29
  11. Study on Culture in Vitro and Induction of Test-tube Bulb of Oriental Lily,S682.29
  12. Study on Agrobacterium-mediated NPR1 Gene Transformation of Lily,S682.29
  13. Studies on Cross-incompatibility between Distantly Related of Lily and the Methods to Overcome,S682.29
  14. Lily isolated bulb formation,S644.1
  15. Occurrence of Lily Virus Disease and Detection Methods of Virus,S436.82
  16. The Studies on Propagation of High Quality Bulbs in Oriental Hybrid Lily,S682.29
  17. Study on Selection of Growing Subsrate for Lily,S682.29
  18. Bulb Propagation Area Test of Ornamental Lily,S682.29
  19. Identification of Lily Viruses Using Oligonucleotide Microarrys,S436.8
  20. The Study of Main Cultivation Techniques of Edible Lily in Mountainous Areas in the North of Guangdong,S644.1

CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Flower bulbs classes > Other
© 2012 www.DissertationTopic.Net  Mobile